Quantification of Regional Intrapulmonary Oxygen Partial Pressure Evolution during Apnea by He MRI

We present a new method to determine in vivo the temporal evolution of intrapulmonary oxygen concentrations by functional lung imaging with hyperpolarized ³Helium (³ ). Single-breath, single-bolus visualization of ³ administered to the airspaces is used to analyze nuclear spin relaxation caused by the local oxygen partial pressure p_O2(t). We model the dynamics of hyperpolarization in the lung by rate equations. Based hereupon, a double acquisition technique is presented to separate depolarization by RF pulses and oxygen induced relaxation. It permits the determination of p_O2 with a high accuracy of up to 3% with simultaneous flip angle calibration using no additional input parameters. The time course of p_O2 during short periods of breathholding is found to be linear in a pig as well as in a human volunteer. We also measured the wall relaxation time in the lung and deduced a lower limit of 4.3 min.     

Wireless retrospective gating: Application to cine cardiac imaging

A new “wireless” method of cardiac imaging is introduced, which, unlike ECG triggering, allows imaging the heart at end-diastole, and greatly reduces smearing artifacts in the phase-encoding direction. It is an improvement over ECG-driven retrospective gating, in that patients with poor ECGs can be imaged. This method extends the applicability of cardiac imaging, and since it requires no physiological monitoring hardware, can be implemented easily on any MR imager. The images produced by this method are superior to those from ECG triggering, especially when viewed in a “cine” loop. The technique described herein is, furthermore, extendable to any area where periodic or quasi-periodic motion is a problem.     

Gadolinium-DTPA-enhanced MRI of intraocular tumors

The value of gadolinium-enhanced MRI in 30 patients with intraocular lesions has been evaluated. Seventeen patients had a uveal melanoma, two a ciliary body melanoma, three had uveal metastases, one lymphoma, four had senile macula degenerations, and three uveal nevi. Twelve of 17 patients with melanoma were followed up by MRI after ruthenium plaque therapy on 2–4 occasions. Melanomas showed high precontrast signal intensities and only a slight enhancement after intravenous Gd-DTPA was given. After ruthenium plaque therapy precontrast signal intensities (SI) decreased while a moderate signal increase on postcontrast scans was noted. Scars or tumor residues were better delineated on enhanced images. All other tumors than melanotic melanomas showed low SI on precontrast scans and a high signal increase after Gd-DTPA administration. Small amelanotic tumors were better delineated on postcontrast scans. In addition Gd-DTPA-enhanced MRI allowed differentiation between tumor and hemorrhage. No signal increase after Gd-DTPA application was seen in subretinal or vitreous hemorrhages of varying ages.     

用MRI无损检测胆囊中的胆汁成

为了探索用磁共振成象方法无损伤检测胆囊中的胆汁成分的可能性,我们用0.5T的磁共振成象仪,对38例禁食期胆囊疾病患者(12例急性无并发症胆囊炎及胆结石,1例化浓性胆囊炎,2例急性出血性胆囊炎,14例慢性胆囊炎及胆结石,5例胆囊息肉,4例障碍性黄疸进行了轴向腹部胆囊部位T1-加权自旋-回波成象.结果表明:禁食或胆囊疾病患者胆汁中胆固醇(p=0.014),脂肪酸(p=0.001),和铁(p<0.001)的浓度对T₁-加权成象(TR/TE=620/25ms)的肝-胆信号强度比有明显的负影响,同时发现总蛋白质,总胆红素及Na⁺,K⁺,Cl^-,Mg⁺⁺离子的浓度对肝-胆MRI信号强度比无显著影响,由此可见,T₁-加权MRI中的肝-胆信号强度比反映了胆囊中的胆汁成分,其中影响肝-胆信号强度比的最重要因素是胆囊中胆汁所含胆固醇、脂肪酸和铁的浓度.     

Establishing norms for age-related changes in proton T1 of human brain tissue in vivo

The goal of this study was to determine the expected normal range of variation in spin-lattice relaxation time (T₁) of brain tissue in vivo, as a function of age. A previously validated precise and accurate inversion recovery method was used to map T₁ transversely, at the level of the basal ganglia, in a study population of 115 healthy subjects (ages 4 to 72; 57 male and 58 female). Least-squares regression analysis shows that T₁ varied as a function of age in pulvinar nucleus (R² = 56%), anterior thalamus (R² = 51%), caudate (R² = 50%), frontal white matter (R² = 47%), optic radiation (R² = 39%), putamen (R² = 36%), genu (R² = 22%), occipital white matter (R² = 20%) (all p < 0.0001), and cortical gray matter (R² = 53%) (p < 0.001). There were no significant differences in T₁ between men and women. T₁ declines throughout adolescence and early adulthood, to achieve a minimum value in the fourth to sixth decade of life, then T₁ begins to increase. Quantitative magnetic resonance imaging provides evidence that brain tissue continues to change throughout the lifespan among healthy subjects with no neurologic deficits. Age-related changes follow a strikingly different schedule in different brain tissues; white matter tracts tend to reach a minimum T₁ value, and to increase again, sooner than do gray matter tracts. Such normative data may prove useful for the early detection of brain pathology in patients.     

NMR flow imaging of Newtonian liquids and a concentrated suspension through an axisymmetric sudden contraction

Using nuclear magnetic resonance (NMR) flow imaging to examine fluid motions at constant velocities or flows that change relatively slowly has been well-documented in the literature. Application of this technique to accelerative flows, on the other hand, has been limited. This study reports the use of an NMR flow imaging method, for which acceleration is not explicitly compensated in the NMR pulse sequence, to measure axial and radial fluid motions during flow through an axisymmetric sudden contraction. In this flow geometry, both velocity and acceleration are spatially dependent. The flow contraction ratio was 2:1. The method was first applied to examine Newtonian liquids at low and high Reynolds numbers under laminar flow conditions. The measured axial and radial velocity profiles, without accounting for acceleration effects in the data analysis, across the contraction are in excellent qualitative agreement with previous experimental data and theoretical calculations reported in the literature. Quantitative comparison of the axial and radial velocities with numerical results indicates that the maximum error from acceleration effects is about 10%. The method has also been used to examine the flow of a concentrated suspension (50% by volume of solid particles) through the contraction. The flow kinematics of the suspension at creeping flow conditions appear to mimic those of the Newtonian fluid with some slight differences. NMR images taken immediately following the cessation of flow suggest a slight degree of particle migration toward the center of the pipe downstream of the contraction.     

Preparation of highly dispersible and tumor-accumulative, iron oxide nanoparticles Multi-point anchoring of PEG-b-poly(4-vinylbenzylphosphonate) improves performance significantly

This paper describes the preparation of iron oxide nanoparticles, surface of which was coated with extremely high immobilization stability and relatively higher density of poly(ethylene glycol) (PEG), which are referred to as PEG protected iron oxide nanoparticles (PEG-PIONs). The PEG-PIONs were obtained through alkali coprecipitation of iron salts in the presence of the PEG-poly(4-vinylbenzylphosphonate) block copolymer (PEG-b-PVBP). In this system, PEG-b-PVBP served as a surface coating that was bound to the iron oxide surface via multipoint anchoring of the phosphonate groups in the PVBP segment of PEG-b-PVBP. The binding of PEG-b-PVBP onto the iron oxide nanoparticle surface and the subsequent formation of a PEG brush layer were proved by FT-IR, zeta potential, and thermogravimetric measurements. The surface PEG-chain density of the PEG-PIONs varied depending on the [PEG-b-PVBP]/[iron salts] feed-weight ratio in the coprecipitation reaction. PEG-PIONs prepared at an optimal feed-weight ratio in this study showed a high surface PEG-chain surface density (≈0.8 chainsnm(-2)) and small hydrodynamic diameter (<50 nm). Furthermore, these PEG-PIONs could be dispersed in phosphate-buffered saline (PBS) that contains 10% serum without any change in their hydrodynamic diameters over a period of one week, indicating that PEG-PIONs would provide high dispersion stability under in vivo physiological conditions as well as excellent anti-biofouling properties. In fact we have confirmed the prolong blood circulation time and facilitate tumor accumulation (more than 15% IDg(-1) tumor) of PEG-PIONs without the aid of any target ligand in mouse tumor models. The majority of the PEG-PIONs accumulated in the tumor by 96 h after administration, whereas those in normal tissues were smoothly eliminated by 96 h, proving the enhancement of tumor selectivity in the PEG-PION localization. The results obtained here strongly suggest that originally synthesized PEG-b-PVBP, having multipoint anchoring character by the phosphonate groups, is rational design for improvement in nanoparticle as in vivo application. Two major points, viz., extremely stable anchoring character and dense PEG chains tethered on the nanoparticle surface, worked simultaneously to become PEG-PIONs as an ideal biomedical devices intact for prolonged periods in harsh biological environments.     

Folate-targeted optical and magnetic resonance dualmodality PCL-<Emphasis Type="Italic">b</Emphasis>-PEG micelles for tumor imaging

A biodegradable tumor targeting nano-probe based on poly（ε-caprolactone）-b-poly（ethylene glycol）block copolymer（PCL-b-PEG）micelle functionalized with a magnetic resonance imaging（MRI）contrast agent diethylenetriaminepentaacetic acid-gadolinium(DTPA-Gd³⁺)on the shell and a near-infrared（NIR）dye in the core for magnetic resonance and optical dual-modality imaging was prepared.The longitudinal relaxivity（r₁）of the PCL-b-PEG-DTPA -Gd³⁺micelle was 13.4（mmol/L）^-1s^-1,three folds of that of DTPA-Gd³⁺,and higher than that of many polymeric contrast agents with similar structures.The in vivo optical imaging of a nude mouse bearing xenografted breast tumor showed that the dual-modality micelle preferentially accumulated in the tumor via the folic acid-mediated active targeting and the passive accumulation by the enhanced permeability and retention（EPR）effect.The results indicated that the dualmodality micelle is a promising nano-probe for cancer detection and diagnosis.     

A pyrophosphate-responsive gadolinium(III) MRI contrast agent

This study shows that the relaxivity and optical properties of functionalised lanthanide‐DTPA‐bis‐amide complexes (lanthanide=Gd³⁺ and Eu³⁺, DTPA=diethylene triamine pentaacetic acid) can be successfully modulated by addition of specific anions, without direct Ln³⁺/anion coordination. Zinc(II)‐dipicolylamine moieties, which are known to bind strongly to phosphates, were introduced in the amide “arms” of these ligands, and the interaction of the resulting Gd–Zn₂ complexes with a range of anions was screened by using indicator displacement assays (IDAs). Considerable selectivity for polyphosphorylated species (such as pyrophosphate and adenosine‐5′‐triphosphate (ATP)) over a range of other anions (including monophosphorylated anions) was apparent. In addition, we show that pyrophosphate modulates the relaxivity of the gadolinium(III) complex, this modulation being sufficiently large to be observed in imaging experiments. To establish the binding mode of the pyrophosphate and gain insight into the origin of the relaxometric modulation, a series of studies including UV/Vis and emission spectroscopy, luminescence lifetime measurements in H₂O and D₂O, ¹⁷O and ³¹P NMR spectroscopy and nuclear magnetic resonance dispersion (NMRD) studies were carried out.