Two new polysaccharide‐derived chiral selectors, namely, 6‐azido‐6‐deoxy‐3,5‐dimethylphenylcarbamoylated amylose and 6‐azido‐6‐deoxy‐3,5‐dimethylphenyl carbamoylated cellulose, were synthesized under homogeneous conditions and immobilized onto aminized silica gel by the Staudinger reaction, resulting in two new immobilized polysaccharide chiral stationary phases (CSPs). Their enantioseparation performances were investigated under normal‐phase mode by HPLC. Among 17 analytes, baseline separations of 12 pairs of enantiomers are achieved on the immobilized cellulose CSP, which demonstrates that this new cellulose material exhibits almost the same enantioseparation performance as the coated cellulose CSP. In addition, the amylose‐derived CSP presents limited enantiorecognition ability but certain complementarity with the immobilized and coated cellulose‐based materials. Neither metolachlor nor paclitaxel side chain acids are separated on two cellulose‐derived CSPs, but effective separations are obtained on the immobilized amylose column. 相似文献
Invertase or β-d-Fructofuranosidase (E.C.3.2.1.26) was extracted from Cucumis melo. L. fruit (Family-Cucurbitaceae). Soluble, plant invertase enzyme was immobilized in novel composite of agarose-guar gum biopolymer matrix in the form of hydrophilic, porous membranes. The immobilized invertase was characterized for sucrose hydrolytic activity and leakage from the matrix support. The efficiency of immobilization was found to be 91% with negligible leaching. The kinetic parameters Km and Vmax for free and immobilized invertase were also determined. Immobilized invertase was optimally active in the wide pH range of 4.5-6.5. The immobilization process also enhanced the thermal stability of enzyme up to 65 °C. Immobilized invertase membranes showed excellent storage stability with shelf life of 110 days. Entrapped invertase showed better operational stability and reusability up to 12 cycles. The fluorescence spectra of the composite membranes were studied and compared with that of soluble enzyme. All these characteristics of the immobilized invertase membranes make them suitable for the fabrication of biosensors. 相似文献
A use of sulfonate ester as a linker in synthesis of w-aminoalkanols was reporte.Diols were tethered onto polystyryl sulfonyl chloride resin,yielding sulfonate resins(2).After cleaved by diethyl amine,diisopropylamine and propylamine respectively,three w-aminoalkanlos were obtained. 相似文献
Microbial treatments of waste water can be done in membrane reactors. A membrane installed outside the reactor is used to separate bacteria from the treated effluent.
A new membrane reactor concept is presented. The separation membrane is introduced in the reactor and not outside as in a normal one. The membrane plays the role of a separator of two streams and is used at the same time as the immobilizing support for the bacteria.
The reactor keeps the bacteria active via a specific nutrient stream that is provided on one side of the membrane. The bacteria grow in and on the membrane where they form an active biofilm. The bacteria can treat the effluent on one side and can be kept active via the nutrient stream at the other side without contamination of the effluent by the nutrient.
In this work, the performance of the BICMER (Bacteria Immobilized Composite MEmbrane Reactor) is demonstrated via treatments of effluents containing heavy metals or organic xenobiotics.
For heavy metal removal Alcaligenes eutrophus CH34 bacteria were used. These bacteria induce a metal bioprecipitation process that results in the formation of crystalline metal carbonates, which are recovered on a separate column in the reactor. In this way metals can be recovered without disturbing the biofilm on the membrane. Metals such as Cd, Zn, Cu, Pb and Y can be reduced to less than 50 ppb. The metals Co, Ni, Pd and Ge are reduced to below 100 ppb.
For organic xenobiotics Alcaligenes eutrophus AE1308 bacteria or other strains (depending on the xenobiotic to be degraded) were used. This strain degrades the xenobiotic 3-chlorobenzoate (Cba) and 2,4-dichlorophenoxyacetic acid to CO2, H2O and chloride). Concentrations of 3 mM Cba could be reduced to less than 0.1 mM. For other toxic organic compounds, different biodegrading strains need to be used. 相似文献
The recently developed ‘protective plate’ method offers the possibility to include protein layers into a Langmuir–Blodgett (LB) assembly without contact of protein molecules with the air–water interface thus avoiding their denaturation. In the present work, this technique was applied for the deposition of biocatalysts with active layers of penicillin G acylase (PGA), an enzyme widely used for medicine production. Easy selection of LB and adsorbed layers resulted in the creation of appropriate environments for the preservation of PGA functions. Two structures were tested regarding such performances as the enzymatic activity value and the level of PGA detachment in aqueous solutions. It was shown that they satisfy the requirements for biocatalytic applications. The enzymatic activity of PGA monolayer incorporated into the film reached 25–30% of the activity value of the equivalent amount of protein in the solution, which is a good result for an immobilized enzyme. Further modification of the deposition procedure resulted in increasing the effective activity per unit of the substrate surface due to adsorption of a thicker protein layer in one cycle. Probably, a three-dimensional frame-like structure was formed, which allowed the substrate molecules to penetrate into the film. The enzymatic activity of such films per unit of the substrate surface was 20–25 times higher than that of the assemblies with one adsorbed monolayer. Finally, the method is proposed of biocatalytic LB assembly deposition onto flexible supports of practically unlimited length without the exposure of protein layer to air medium. 相似文献
The 3,5-dichlorophenylcarbamates (2) of cellulose bearing a small amount of 3-(triethoxysilyl)propyl residues were synthesized by a one-pot process and immobilized onto a silica gel through intermolecular polycondensation of the triethoxysilyl groups. The obtained cellulose derivatives were characterized by (1) H NMR and elemental analysis (EA), and their recognition abilities were evaluated by high-performance liquid chromatography (HPLC). The cellulose derivatives containing about 1-5% of the 3-(triethoxysilyl)propyl residue were efficiently immobilized with a high chiral recognition ability. The immobilized chiral packing materials (CPMs) could be used with the eluents containing chloroform and tetrahydrofuran (THF), which cannot be used with the conventional coated-type chiral packing materials. By using these eluents, the chiral recognition for many racemates was improved. 相似文献
Seven wild-type microorganism strains were used to reduce ethyl 3-oxohexanoate to ethyl (R)-3-hydroxyhexanoate. Free cells of Kluyveromyces marxianus and Aspergillus niger led to higher than 99% of conversion with higher than 99% ee. After immobilization in calcium alginate spheres, cells of K. marxianus exhibited high enantioselectivity (>99% ee) and conversion level (99%) within 24 h even if substrate was added at concentration of 10 g/L (62 mM). 相似文献
Sulfotransferases are categorized as phase II metabolic enzymes. Human sulfotransferase 1A1 (SULT1A1) is involved in the sulfonation of xenobiotics with aid from the cofactor 3'‐phosphoadenosine‐5'‐phosphosulfate that acts as a sulfonate donor. In this study, we have attempted to immobilize SULT1A1 on magnetic microparticles (MMs). Different functionalized MMs were used to immobilize SULT1A1 and their enzyme activity was compared to the control (enzyme in solution). Paracetamol was used as model substrate. Separation of paracetamol and paracetamol sulfate by CE‐UV was optimized and validated. MMs with epoxy based immobilization of SULT1A1 showed better enzyme activity. Hence, they were tested for repeated usage to allow their implementation for the development of a CE immobilized micro enzyme reactor. 相似文献
Nickel(II) tetradentate Schiff-base complexes of N,N′-(bis(pyridin-2-yl)formylidene)ethane-1,2-diamine (L1), N,N′-(bis(pyridin-2-yl)formylidene)cyclohexane-1,2-diamine (L2), N,N′-(bis(pyridin-2-yl)formylidene)benzene-1,2-diamine (L3), N,N′-(bis(pyridin-2-yl)formylidene)meso-stilben-1,2-diamine (L4), and N,N′-(bis(pyridin-2-yl)formylidene)propane-1,3-diamine (L5) were synthesized, characterized, and immobilized on sodium montmorillonite. The complexes were characterized by IR spectroscopy, diffuse reflectance spectra (DRS), and atomic absorption spectroscopy (AAS). IR and DRS data of the heterogeneous catalysts show that the Ni(II) complexes were physically entrapped within the sodium montmorillonite clay. The supported complexes show good catalytic activity for the epoxidation of cyclooctene using tert-butylhydroperoxide (TBHP) as oxygen source in acetonitrile. The Ni-catalyzed oxidation proceeds with 62.3% selectivity for epoxidation with 69% conversion for supported [Ni(L5)]. 相似文献
Engineering metal-organic frameworks(MOF) for heterogeneous catalysts have been of extreme interest since they have large pore size within the crystalline framework and well defined pore architecture. Ni-containing MOF Ni2(3,5-Pydc)2(H2O)8·2H2O(1·H2O) was prepared by solvothermal method from 3,5-pyridinedicarboxylic acid, D-camphoric acid and Ni(NO3)2·6H2O in dimethylformamide(DMF)/water(volume ratio 2:1). And two gold and silver functionalized 1·H2O catalysts were prepared by impregnation method. Catalysts 2.53%Au/MOF and 4.23%Ag/MOF were in-depth characterized by single crystal X-ray diffraction, powder X-ray diffraction(PXRD), thermogravimetric analysis(TGA), transmission electron microscopy(TEM), and inductively coupled plasma-atomic emission spectroscopy(ICP-AES). Their catalytic performance was examined in one-pot synthesis of structurally divergent propargylamines via three component coupling of aldehyde, alkyne, and amine(A3) in 1,4-dioxane. The results show that the catalysts all displayed high reactivities, and a selectivity of 100% for propargylamines. Catalysts 2.53%Au/MOF and 4.23%Ag/MOF have proved to be applicable to a wide range of substrates. Catalysts 2.53%Au/MOF and 4.23%Ag/MOF can be easily recycled and used repetitively at least 3 times with a slight drop in activity. These features render the catalysts particularly attractive in the practice of propargylamines synthesis in an environmentally friendly manner. 相似文献