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81.
Henri de Maissin Philipp R. Groß Obaid Mohiuddin Dr. Moritz Weigt Luca Nagel Marvin Herzog Zirun Wang Robert Willing Dr. Wilfried Reichardt Dr. Martin Pichotka Dr. Lisa Heß Prof. Dr. Thomas Reinheckel Prof. Dr. Henning J. Jessen Prof. Dr. Robert Zeiser Prof. Dr. Michael Bock Prof. Dominik von Elverfeldt Prof. Dr. Maxim Zaitsev Dr. Sergey Korchak Dr. Stefan Glöggler Prof. Dr. Jan-Bernd Hövener Prof. Dr. Eduard Y. Chekmenev Prof. Dr. Franz Schilling Dr. Stephan Knecht Dr. Andreas B. Schmidt 《Angewandte Chemie (International ed. in English)》2023,62(36):e202306654
82.
Edward W. Randall Andrei A. Samoilenko Riqiang Fu 《Solid state nuclear magnetic resonance》1999,14(3-4):173-179
We have observed Hahn-echoes resulting from Powles–Mansfield pulse-sequences for a variety of nitrogen containing solids which exhibit a range of 14N electric quadrupole coupling constants (Cq) from 0 to 4.9 MHz. Long echo-decays were frequently obtained which allowed the collection of many echoes in one echo-train, so that the method of Long Echo-Train Summation (LETS), could be used to accumulate signal. A one-dimensional STRAFI-profile is shown for solid ammonium nitrate. The spatial resolution is discussed as a function of Cq. 相似文献
83.
To develop a small molecule-based biomarker for in vivo apoptosis imaging, a dansyled amino acid derivative (BNSBA) was designed and synthesized in good yield. The biological evaluation demonstrated that BNSBA selectively binds to apoptotic cancer cells and is localized within the cytoplasm of cells that bound annexin V on the plasma membrane. 相似文献
84.
M. Mirasoli M. Guardigli P. Simoni S. Venturoli S. Ambretti M. Musiani A. Roda 《Analytical and bioanalytical chemistry》2009,394(4):981-987
Classification of cervical intraepithelial neoplasia (CIN) lesions in low-grade (CIN1) or high-grade (CIN2-3) ones is crucial
for optimal patient management, but current histological diagnosis on bioptic samples is often hampered by inter-observer
variability. To allow objective classification, we have exploited the peculiar characteristics of chemiluminescence detection,
such as high sensitivity and easy quantification of the luminescence signal, to perform sequentially in the same tissue section
both an immunohistochemical quantitative detection of p16INK4A (a protein marker of high-grade CIN lesions) and an in situ hybridization for human papillomavirus (generally accepted as
a necessary but insufficient cause of cervical carcinoma). Different label enzymes (alkaline phosphatase and horseradish peroxidase)
were employed in order to avoid any interference between the two assays, and quantitative chemiluminescence image analysis
was used to obtain objective evaluation of sample positivity. The multiplexed method allowed detection of two complementary
biomarkers and provided discrimination between different lesions (non-neoplastic, low-grade and high-grade CIN). This assay
might thus represent an accurate and objective diagnostic test providing important information for counseling, selection of
therapy and follow up after surgical treatment. 相似文献
85.
CdTe quantum dots (QDs), capped with mercaptopropionic acid (MPA), were synthesized and the variation of their fluorescence properties (steady state and lifetime) with pH was assessed in solution and when immobilized in a sol-gel host. Three different sizes of CdTe QDs with excited state lifetimes ranging from 42 to 48 ns and with emission maximum at 540 nm (QD540), 580 nm (QD580) and 625 nm (QD625) were selected. The solution pH affects the maximum emission wavelength (shifts to higher wavelengths of 23, 24 and 27 nm for QD540, QD580 and QD625, respectively), the excited state lifetime and the fluorescence intensity in a reversible way. Linearization of the maximum emission wavelength variation with the pH allows the estimation of an apparent ionization constant (pKa) for each QD: 6.5 ± 0.1 (QD540), 6.1 ± 0.5 (QD580) and 5.4 ± 0.3 (QD625). The variation of the QDs fluorescence properties was further explored using confocal laser scanning microscopy allowing the implementation of a new calibration method for pH imaging in solution. QDs were successfully immobilized on the tip of an optical fiber by dip-coating using sol-gel procedure. The immobilized QDs showed a similar pH behaviour to the one observed in solution and an apparent lifetime of 80, 68 and 99 ns, respectively. The proposed QDs based methodology can be successfully used to monitor pH using wavelength encoded data in imaging and fiber optic sensing applications. 相似文献
86.
核酸适配体是利用体外筛选技术,即指数富集的配体系统进化技术(SELEX),从核酸分子文库中得到的寡核苷酸片段。其与靶标物有很高的特异性和亲和力,将适配体作为识别单元的生物传感研究以及适配体偶联成像试剂的生物体内外成像研究在临床诊断中有很大的应用前景,此外,适配体靶向癌细胞或组织的治疗方法相比传统化学治疗副作用更小,在临床上也有极大的应用前景。本文综述了适配体目前在癌症诊断和靶向治疗两个方面的研究进展,并分析现阶段存在的问题以及面临的挑战。 相似文献
87.
88.
红外探测器成像实验研究 总被引:1,自引:0,他引:1
红外探测器广泛应用于多光谱成像系统中,文章提出并建立了一种红外线阵时间延迟积分探测器的成像实验系统,阐述了成像系统的工作原理,对影响成像质量的两个主要因素电子稳像与自动对焦进行了深入分析。利用高精度直流测速机确定了转台转速与探测器的行转移频率,采用卡尔曼滤波算法滤除了测速机带来的噪声,提高了速度匹配精度。在分析比较红外相机四种检焦方法的基础上采用视频信号幅度法进行检调焦。在国家光学机械产品质量监督检验测试中心实地搭建了成像实验系统,并分别对5.3,6.4和9.2mm宽度靶标成像。实验结果表明,采集到的原始靶标纹理清晰,照相分辨率达到了每毫米11.3对线,达到了实验预期目的。 相似文献
90.
扩频雷达探测一阶海洋回波 总被引:1,自引:1,他引:0
利用扩频通信良好的抗干扰性和低功率谱密度下工作的特点,本文把扩频技术引入高频海洋探测雷达来探测海洋一阶回波,研究结果表明,该技术对回波质量无不良影响,同时可降低雷达发射功率,增加雷达探测距离. 相似文献