Molecular engineering and biomedical applications of ultra-sensitive fluorescent probe for Ag+

A series of probes KJ-x (x = 1−3) with carbon chains of different lengths based on the matrix of rhodamine B were engineered to detect Ag⁺ in aqueous solution in this work. Among them, KJ-1 is selected as the best option after in vitro investigation in view of its most sensitive and rapid response to Ag⁺, whose possible sensing mechanism is studied by experimental investigation and theoretical calculation. To identify the practical application of the probe, the detection of Ag⁺ in nonantibiotic fungicide Silver&Health and differentiation between normal hepatocytes and hepatoma cells using confocal imaging was conducted.     

Naphthalimide-rhodamine based fluorescent probe for ratiometric sensing of cellular pH

The pH values of lysosomes in cancer cells is slightly lower than that in normal cells, which can be used to distinguish cancer cells from normal cells. According to this, a naphthalimide-rhodamine based fluorescent probe(hereafter referred to as RBN) with a pK_a of 4.20 was designed and synthesized for ratiometric sensing of cellular pH via fluorescence resonance energy transfer(FRET), which can respond to different pH precisely through ratiometric fluorescence intensity(Ⅰ_(577)/Ⅰ_(540)). RBN can be employed to distinguish cancer cells from normal cells on the basis of different fluorescent response, in particular, RBN showed excellent water solubility and low cell toxicity, all these are quite significant for potential application in cancer diagnose and therapy.     

激光雷达干扰效果评估方法研究

研究了光电干扰对测距和跟踪测量激光雷达干扰效果的评估方法.在深入分析激光雷达光电干扰机理的基础上,提出了相应的干扰效果评估准则.对激光测距机的距离欺骗干扰效果采用测距误差-干扰成功率准则或准测率准则定量评估,对测距机的激光盲干扰效果依据盲激光对测距准确度、测距功能的影响程度进行评估.对于跟踪测量激光雷达,根据实施干扰后其跟踪误差是否超出正常跟踪准确度的三倍判定一次干扰是否有效,再依据多次试验的干扰成功率的大小评估干扰效果.     

Recent Development of DNA-modified AIEgen Probes for Biomedical Application

A variety of DNA-based probes are utilized for the detections of multiple analytes and DNA nanotechnology has been thriving for recent decades and achieving numerous nanostructures,mainly focusing on DNA morphology modulation and multifunctional systems engineered into to the complicated works.Among the numerous detections,fluorescence method is a non-invasive,highly selective and sensitive means for varieties of applications,but their emissions are often compromised by the aggregation-caused quenching(ACQ)effect,which weakens their applications.The aggregation induced emission luminogens(AIEgens)are created with non emissive or weakly emissive in a low concentration but emit strong fluorescence in a high concentration with aggregated states.Herein,numerous functionalized AIEgens have been emerged and used for detection and imaging and DNA-modified AIEgen probes are introduced.In this vein,here we report the progress on DNA-modified AIEgen probes in recent years and highlight their conjugation strategies including covalent bonding,electrostatic interaction and their applications of biosensing.Moreover,multiple DNA strands are needed to introduce into the DNA-modified AIEgen probes for more purposes.At the end,some challenges are mentioned to discuss the new trend of DNA-modified AIEgen probes.     

Adaptive image enhancement based on clustering of wavelet coefficients for infrared sea surveillance systems

Most of the techniques developed for infrared (IR) image enhancement (IE) depend heavily on the scene, environmental conditions, and the properties of the imaging system. So, with a set of predefined scenario properties, a content-based IR-IE technique can be developed for better situational awareness. This study proposes an adaptive IR-IE technique based on clustering of wavelet coefficients of an image for sea surveillance systems. Discrete wavelet transform (DWT) of an image is computed and feature vectors are constructed from subband images. Clustering operation is applied to group similar feature vectors that belong to different scene components such as target or background. Depending on the feature vectors, a weight is assigned to each cluster and these weights are used to compute gain matrices which are used to multiply wavelet coefficients for the enhancement of the original image. Enhancement results are presented and a comparison of the performance of the proposed algorithm is given through subjective tests with other well known frequency and histogram based enhancement techniques. The proposed algorithm outperforms previous ones in the truthfulness, detail visibility of the target, artificiality, and total quality criteria, while providing an acceptable computational load.     

Wavelength selection of bidirectional laser transmission based on Monte Carlo method

The laser detection technology in uncertain and dynamic environments is of utmost importance in many fields. A model of transient radiative transfer of bidirectional path laser based on Monte Carlo method is developed to investigate the optimum wavelength of active detector at complex atmospheric conditions. The radiative parameters of atmosphere are calculated by HITRAN database and Mie theory at several typical atmospheric conditions including the standard atmosphere, urban aerosol, and radiation fog. Transmission characteristics for five spectral bands at the above atmospheric conditions are calculated by this model. The optimal transmission ability occurred in bands 0.2–0.5, 1.4–1.6, and 0.75–1.25 μm on the condition of standard atmosphere, urban aerosol, and radiation fog, respectively. All results provide effective reference and basic support for choosing the optimal spectral band for active detection.     

基于主成分的改进雷达图及其在综合评价中的应用

本文从可视化角度出发,在总结传统可视化方法因优点与不足的基础上,将主成分分析、因子分析与雷达图结合,改进了传统的可视化方法。并将新的可视化方法引入到综合评价中,提出了一种基于雷达图面积的综合评价模型,实例验证该种方法切实有效。     

Ocean internal waves interpreted as oscillation travelling waves in consideration of ocean dissipation

Most studies of the synthetic aperture radar remote sensing of ocean internal waves are based on the solitary wave solutions of the Korteweg-de Vries （KdV） equation, and the dissipative term in the KdV equation is not taken into account. However, the dissipative term is very important, both in the synthetic aperture radar images and in ocean models. In this paper, the traveling-wave structure to characterize the ocean internal wave phenomenon is modeled, the results of numerical experiments are advanced, and a theoretical hypothesis of the traveling wave to retrieve the ocean internal wave parameters in the synthetic aperture radar images is introduced.     

Segmentation of synthetic aperture radar image using multiscale information measure-based spectral clustering

A multiscale information measure （MIM）, calculable from per-pixel wavelet coefficients, but relying on global statistics of synthetic aperture radar （SAR） image, is proposed. It fully exploits the variations in speckle pattern when the image resolution varies from course to fine, thus it can capture the intrinsic texture of the scene backscatter and the texture due to speckle simultaneously. Graph spectral segmen- tation methods based on MIM and the usual similarity measure are carried out on two real SAR images. Experimental results show that MIM can characterize texture information of SAR image more effectively than the commonly used similarity measure.     

Detection of myocardial viability by low-dose dobutamine Cine MR imaging

The purpose of this work was to test the diagnostic value of dobutamine stress magnetic resonance imaging (MRI) for predicting recovery of regional myocardial contractility after revascularization. Cardiac wall motion abnormalities are due to either non-viable and/or scarred, or viable, but hibernating, myocardial tissue. Dobutamine stress leads to increased systolic wall thickening only in viable myocardium. Twenty-five patients with akinetic or dyskinetic myocardial regions were examined with a Cine FLASH-2D sequence at rest and during dobutamine stress (10 μg/kg/min). Patients were re-examined at rest 3, and in case of persisting wall motion defects, 6 months after revascularization. Criterion of viability was increasing end-systolic wall thickening during stress and/or at follow-up. Akinetic regions related either to the LAD (n = 19) or to the RCA (n = 6) were judged viable if >=50% of the affected segments improved. MR studies were completed in all subjects without arrhythmia or need for early terminations due to symptoms. Sensitivity, specificity, and positive predictive value for the prediction of myocardial viability were 61%, 90%, and 87% for the segment-related analysis, and 76%, 100%, and 100% for the patient-related analysis based on coronary artery distribution, respectively. Dobutamine stress MRI allows to predict global functional recovery of akinetic myocardial regions after revascularization with a high positive predictive value and high specificity.