Analysis and modeling of radiometric error caused by imaging blur in optical remote sensing systems

Imaging blur changes the digital output values of imaging systems. It leads to radiometric errors when the system is used for measurement. In this paper, we focus on the radiometric error due to imaging blur in remote sensing imaging systems. First, in accordance with the radiometric response calibration of imaging systems, we provide a theoretical analysis on the evaluation standard of radiometric errors caused by imaging blur. Then, we build a radiometric error model for imaging blur based on the natural stochastic fractal characteristics of remote sensing images. Finally, we verify the model by simulations and physical defocus experiments. The simulation results show that the modeling estimation result approaches to the simulation computation. The maximum difference of relative MSE (Mean Squared Error) between simulation computation and modeling estimation can achieve 1.6%. The physical experimental results show that the maximum difference of relative MSE between experimental results and modeling estimation is only 1.29% under experimental conditions. Simulations and experiments demonstrate that the proposed model is correct, which can be used to estimate the radiometric error caused by imaging blur in remote sensing images. This research is of great importance for radiometric measurement system evaluation and application.     

3D photon counting imaging at 1550 nm with 1.5-GHz sine-wave-gated InGaAs/InP GAPD

We demonstrated a 3D laser imaging system at 1550 nm with a 1.5-GHz sine-wave gated Geiger-mode InGaAs/InP avalanche photodiode (APD). An optical fiber bundle with 100 individual fiber outputs was implemented at the focal plane of the telescope, providing a 2.5-mrad imaging view. The system used single-pixel near-infrared single-photon detector to measure photons at fiber outputs instead of a photon counting array. The 1.5-GHz gated Geiger-mode InGaAs/InP APD with a timing jitter of 290 ps was operated in quasi-continuous mode with detection efficiency of ∼4.3%. We achieved higher than 6-cm surface-to-surface resolution at single-photon level, showing a potential of low-energy and eye-safe laser imaging system for long-distance measurements.     

Detection on micro-Doppler effect based on 1550 nm laser coherent radar

A laser coherent detection system of 1550 nm wavelength was presented, and experimental research on detecting micro-Doppler effect in a dynamic target was developed. In this paper, the return signal in the time domain is decomposed into a set of components in different wavelet-scales by multi-resolution analysis, and the components are associated with the vibrational motions in a target. The micro-Doppler signatures are extracted by applying the reconstruction (inverse wavelet transform). During the course of the final data processing frequency analysis and time–frequency analysis are applied to analyze the vibrational signals and estimate the motion parameters successfully. The experimental results indicate that the micro-Doppler information in a moving can be effectively detected, and tiny vibrational signatures also can be acquired effectively by wavelet multi-resolution analysis and time–frequency analysis.     

Synthesis of gemini surfactants with twelve symmetric fluorine atoms and one singlet F MR signal as novel F MRI agents

A family of fluorinated gemini surfactants derived from perfluoropinacol has been synthesized as novel ¹⁹F magnetic resonance imaging (¹⁹F MRI) agents. These fluorinated surfactants with 12 symmetric fluorine atoms and one singlet ¹⁹F MR peak can be conveniently prepared from perfluoropinacol and oligo(ethylene glycols) on multi-gram scales. Solubility, hydrophilicity (log P), and critical micelle concentration (CMC) measurements of these fluorinated surfactants indicated that high aqueous solubility can be achieved by introducing oligo(ethylene glycols) with appropriate length into perfluoropinacol, i.e., manipulating the fluorine content (F%). One of these fluorinated surfactants with high aqueous solubility and excellent ¹⁹F MR properties has been identified by ¹⁹F MRI phantom experiments as a promising ¹⁹F MRI agent.     

Synthesis and characterization of a new lanthanide based MRI contrast agent,potential and versatile tracer for multimodal imaging

In the present work a modular pathway towards the synthesis of a new versatile MRI contrast agent is reported and its physico-chemical properties are described. Two different functional groups were attached on two arms of the gadolinium 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate (DOTA) in order to get a platform able to bind one probe designed to target specific biological marker and a fluorescent molecule likely to be used for optical imaging. The nuclear magnetic relaxation dispersion (NMRD) profile, the oxygen-17 relaxometric NMR study and stability assessment versus transmetalation of the Gd-complex show that this new contrast agent has a relaxivity and transmetalation stability similar to Gd–DOTA.     

Supramolecular optical sensor arrays for on-site analytical devices

Supramolecular optical chemosensors are useful tools in analytical chemistry for the visualization of molecular recognition information. One advantage is that they can be utilized for array systems to detect multiple analytes. However, chemosensor arrays have been evaluated mainly in the solution phase, which limits a wide range of practical applications. Thus, appropriate solid support materials such as polymer gels and papers are required to broaden the scope of the application of chemosensors as on-site analytical tools. In this review, we summarize the actual approaches for the fabrication of solid-state chemosensor arrays combined with powerful data processing techniques and portable digital recorders for real-world applications.     

Improved Stabilities of Labeling Probes for the Selective Modification of Endogenous Proteins in Living Cells and In Vivo

To date, various affinity-based protein labeling probes have been developed and applied in biological research to modify endogenous proteins in cell lysates and on the cell surface. However, the reactive groups on the labeling probes are also the cause of probe instability and nonselective labeling in a more complex environment, e. g., intracellular and in vivo. Here, we show that labeling probes composed of a sterically stabilized difluorophenyl pivalate can achieve efficient and selective labeling of endogenous proteins on the cell surface, inside living cells and in vivo. As compared with the existing protein labeling probes, probes with the difluorophenyl pivalate exhibit several advantages, including long-term stability in stock solutions, resistance to enzymatic hydrolysis and can be customized easily with diverse fluorophores and protein ligands. With this probe design, endogenous hypoxia biomarker in living cells and nude mice were successfully labeled and validated by in vivo, ex vivo, and immunohistochemistry imaging.