车前草的微量元素含量分析

用电感耦合等离子体发射光谱法测定了湛江地区的车前草中微量元素的含量,发现其Ca、Mn、Zn、Fe、Mg、P、Cu等元素的含量都比较丰富,并讨论了这些有益元素与人体健康的关系。     

Exploration of a ternary deep eutectic solvent of methyltriphenylphosphonium bromide/chalcone/formic acid for the selective recognition of rutin and quercetin in Herba Artemisiae Scopariae

Methyltriphenylphosphonium bromide/chalcone/formic acid, a green ternary deep eutectic solvent, was applied as a functional monomer and dummy template simultaneously in the synthesis of a new molecularly imprinted polymer. Ternary deep eutectic solvent based molecularly imprinted polymers are used as a solid‐phase extraction sorbent in the separation and purification of rutin and quercetin from Herba Artemisiae Scopariae combined with high‐performance liquid chromatography. Fourier transform infrared spectroscopy and field‐emission scanning electron microscopy were applied to characterize the deep eutectic solvent based molecularly imprinted polymers synthesized using different molar ratios of chalcone. The static and competitive adsorption tests were performed to examine the recognition ability of the molecularly imprinted polymers to rutin and quercetin. The ternary deep eutectic solvent consisting of formic acid/chalcone/methyltriphenylphosphonium bromide (1:0.05:0.5) had the best molecular recognition effect. After optimization of the washing solvents (methanol/water, 1:9) and eluting solvents (acetonitrile/acetic acid, 9:1), a reliable analytical method was developed for strong recognition towards rutin and quercetin in Herba Artemisiae Scopariae with satisfactory extraction recoveries (rutin: 92.48%, quercetin: 94.23%). Overall, the chalcone ternary deep eutectic solvent‐based molecularly imprinted polymer coupled with solid‐phase extraction is an effective method for the selective purification of multiple bioactive compounds in complex samples.     

Qualitative and quantitative analysis of chemical constituents in Ardisiae Japonicae Herba

Ardisiae Japonicae Herba is a well‐known traditional Chinese medicine for the treatment of bronchitis conjunctivitis, pneumonia, and trauma. In this work, a high‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry method was first established for the separation and structural identification of the chemical constituents in Ardisiae Japonicae Herba. A total of 15 compounds including coumarins, flavonoid glycosides, and catechins were identified or tentatively characterized based on their chromatographic behaviors and mass spectral fragmentation and by comparisons with the reference standards. Furthermore, a simple high‐performance liquid chromatography with diode array detection method was developed for the simultaneous determination of five major constituents. Results obtained from method validation, including linearity, precision, repeatability, stability, and recovery, showed that the established method was reliable and accurate. Bergenin and quercitrin were found to be the most abundant constituents and could be served as chemical markers for quality control of Ardisiae Japonicae Herba.     

Fabrication of graphene/poly(methyl methacrylate) composite electrode for capillary electrophoretic determination of bioactive constituents in Herba Geranii

This report describes the development and application of a novel graphene/poly(methyl methacrylate) composite electrode as a sensitive amperometric detector of capillary electrophoresis. The composite electrode was fabricated on the basis of the in situ polymerization of a mixture of graphene and prepolymerized methyl methacrylate in the microchannel of a piece of fused silica capillary under heat. SEM, XRD and FT-IR offered insights into the nature of the composite. The results indicated that graphenes were well dispersed in the composite to form an interconnected conducting network. The performance of this unique graphene-based detector has been demonstrated by separating and detecting seven naturally occurring phenolic compounds in Herba Geranii in combination with capillary electrophoresis. The graphene-based detector offered significantly lower operating potentials, substantially enhanced signal-to-noise characteristics, and lower expense of operation. The simplicity and significant performance exhibited by the graphene/poly(methyl methacrylate) composite electrode also indicate great promise for microchip CE, flowing injection analysis, and other microfluidic analysis systems.     

紫花地丁中汞的原子荧光法测定

利用还原气化的非色散型装置，以湿法消解的方式，建立了原子荧光测定紫花地丁中痕量汞的测定方法，同时比较了不同玻璃仪器的洗涤方法和不同的定量方法，试验回收率在86．9％－110．8％之间，误差＜8．5％，通过对实际样品的测定，得到了满意的结果。     

Chemical composition and anti-complement activity of Glechomae Herba collected in different months

Glechomae Herba (GH) is derived from the dried aerial part of Glechoma longituba (Nakai) Kupr., which is harvested from spring to autumn. It has the effects of clearing heat and detoxification. The aim of this paper was to study the chemical composition and the anti-complement activity of GH collected in different months. Ultra-high-performance liquid chromatography coupled to quadrupole time-of-flight tandem mass spectrometry based on predicted compounds screening and diagnostic ion filter strategy was developed for identifying the chemical composition of GH collected in different months. A total of 102 compounds—40 chlorogenic acids (CGAs), 32 phenolic acids, and 30 flavonoids—were reasonably identified in GH. Thirty-four CGAs were discovered in GH for the first time. The correlations between chemical compositions and anti-complement activities of GH collected in different months were analyzed. Phenolic acids and flavonoids were found to be negatively correlated with anti-complement activity, and CGAs were positively correlated with anti-complement activity. At the same time, six CGA standards had obvious anti-complement activity. It was demonstrated that different harvest months had a significant impact on the difference in chemical composition and anti-complement activity of GH. And CGAs might play an important role in the anti-complement activity of GH.     

A comparative study of Mentha arvensis L. and Mentha haplocalyx Briq. by HPLC

We have developed a new method to simultaneously determine five marker compounds in Menthae Herba via HPLC/PDA – including hesperidin (1), rosmarinic acid (2), diosmin (3), didymin (4) and buddleoside (5). The newly developed method was successfully used to analyse for two species (Mentha arvensis L. and Mentha haplocalyx Briq.) of Menthae Herba, and the satisfactory results were obtained from the validation of developed method. The pattern analysis could greatly discriminate between M. arvensis L. and M. haplocalyx Briq. In conclusion, the proposed HPLC/PDA method is suitable for quality evaluation of Menthae Herba.     

Development and Validation of a LC–ESI–MS Assay for Determination of Icariin in Rat Plasma after Administration of Herba Epimedii

This paper reports a sensitive, specific, and stable chromatographic procedure with selective detection (electrospray mass spectrometry in selected-ion-monitoring mode) combined with simple and efficient sample preparation for determination of icariin in rat plasma after administration of Herba Epimedii. Separation of the analyte, possible endogenous compounds, and constituents of Herba Epimedii were accomplished on a 250 mm × 2.0 mm i.d. C₁₈ column by use of a rapid gradient. Ionization of icariin and clarithromycin (internal standard) was achieved by use of the electrospray interface in positive-ion mode. Conditions such as ionization mode, type of organic modifier, eluent additives, and Q-array potential were optimized to achieve good sensitivity and specificity of icariin detection. Response was a linear function of concentration over the range 0.2–20 ng mL⁻¹. The method is accurate and precise; within-batch and between-batch precision (CV) are <15%, and accuracy (RE) is better than ±15%. The method can be used for analysis of icariin in plasma after administration of Herba Epimedii or of traditional Chinese medicinal preparations containing Herba Epimedii.     

Integrated Network Pharmacology Analysis and In Vitro Validation Revealed the Potential Active Components and Underlying Mechanistic Pathways of Herba Patriniae in Colorectal Cancer

Herba Patriniae (HP) are medicinal plants commonly used in colorectal cancer (CRC) patients. In this study, network pharmacology was used to predict the active components and key signaling pathways of HP in CRC. Patrinia heterophylla, one type of HP, was chosen for validation of the network pharmacology analysis. The phytochemical profile of Patrinia heterophylla water extract (PHW) was determined by UHPLC-MS. MTT, RT-PCR, and Western blot assays were performed to evaluate the bioactivities of PHW in colon cancer cells. Results showed that 15 potentially active components of HP interacted with 28 putative targets of CRC in the compound–target network, of which asperglaucide had the highest degree. Furthermore, the ErbB signaling pathway was identified as the pathway mediated by HP with the most potential against CRC. Both RT-PCR and Western blot results showed that PHW significantly downregulated the mRNA and protein levels of EGFR, PI3K, and AKT in HCT116 cells. Asperglaucide, present in PHW, exhibited an anti-migratory effect in HCT116 cells, suggesting that it could be an active component of PHW in CRC treatment. In conclusion, this study has provided the first scientific evidence to support the use of PHW in CRC and paved the way for further research into the underlying mechanisms of PHW against CRC.