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排序方式: 共有363条查询结果,搜索用时 15 毫秒
351.
352.
Dr. Michael Holtmannspötter Eike Wienbeuker Dr. Timo Dellmann Isabelle Watrinet Prof. Dr. Ana J. Garcia-Sáez Prof. Dr. Kai Johnsson Dr. Rainer Kurre Prof. Dr. Jacob Piehler 《Angewandte Chemie (International ed. in English)》2023,62(18):e202219050
Self-labeling enzymes (SLE) such as the HaloTag have emerged as powerful tools in high and super-resolution fluorescence microscopy. Newly developed fluorogenic SLE substrates enable imaging in the presence of excess dye. To exploit this feature for reversible labeling, we engineered two variants of HaloTag7 with restored dehalogenase activity. Kinetic studies in vitro showed different turnover kinetics for reHaloTagS (≈0.006 s−1) and reHaloTagF (≈0.055 s−1). Imaging by confocal and stimulated emission depletion microscopy yielded 3-5-time enhanced photostability of reHaloTag labeling. Prominently, single molecule imaging with reHaloTags enabled controlled and stable labeling density over extended time periods. By combination with structured illumination, simultaneous visualization of single molecule diffusion and organellar dynamics was achieved. These applications highlight the potential of reHaloTag labeling for pushing the limits of advanced fluorescence microscopy techniques. 相似文献
353.
《Particuology》2023
Compared with conventional graphite anode, hard carbons have the potential to make reversible lithium storage below 0 V accessible due to the formation of dendrites is slow. However, under certain conditions of high currents and lithiation depths, the irreversible plated lithium occurs and then results in the capacity losses. Herein, we systematically explore the true reversibility of hard carbon anodes below 0 V. We identify the lithiation boundary parameters that control the reversible capacity of hard carbon anodes. When the boundary capacity is controlled below 400 mAh g−1 with current density below 50 mA g−1, no lithium dendrites are observed during the lithiation process. Compared with the discharge cut-off voltage to 0 V, this boundary provides a nearly twice reversible capacity with the capacity retention of 80% after 172 cycles. The results of characterization and finite element model reveal that the large reversible capacity below 0 V of hard carbon anodes is mainly benefited from the dual effect of lithium intercalation and reversible lithium film. After the lithium intercalation, the over-lithiation induces the quick growth of lithium dendrites, worsening the electrochemical irreversibility. This work enables insights of the potentially low-voltage performance of hard carbons in lithium-ion batteries. 相似文献
354.
Ning Wen Jin Li Dr. Wei Zhang Dr. Ping Li Dr. Xia Yin Dr. Wen Zhang Dr. Hui Wang Prof. Bo Tang 《Angewandte Chemie (International ed. in English)》2023,62(25):e202302161
Monitoring the early stage of atherosclerosis (AS) without plaque formation is of great significance. Herein, we developed a metal organic framework (MOF)-based fluorescence nanoprobe to analyze the progression of AS by assessing the levels of protein phosphorylation and glucose in blood and tissue. The probe was prepared by post-modification of the MOF with iodine (I3−)-rhodamine B (RhB) associate, which realizes the specific recognition of target object through the metal joint ZrIV and I3−-RhB, respectively. We investigated different stages of target object changes in the early non-plaque stage of AS in blood. It was found that the levels of phosphate and glucose in the blood were higher than those of the normal mice. The results of two-photon images showed that early AS mice had higher levels of protein phosphorylation and glucose than that of the normal mice. The present study provides a suitable fluorescence tool for further revealing the pathogenesis and progression of AS. 相似文献
355.
Lorenzo Albertazzi Mike Heilemann 《Angewandte Chemie (International ed. in English)》2023,62(35):e202303390
The exploitation of low-affinity molecular interactions in protein labeling is an emerging topic in optical microscopy. Such non-covalent and low-affinity interactions can be realized with various concepts from chemistry and for different molecule classes, and lead to a constant renewal of fluorescence signals at target sites. Further benefits are a versatile use across microscopy methods, in 3D, live and many-target applications. In recent years, several classes of low-affinity labels were developed and a variety of powerful applications demonstrated. Still, this research field is underdeveloped, while the potential is huge. 相似文献
356.
David A. Pérez Berrocal Dr. Thimmalapura M. Vishwanatha Dr. Daniel Horn-Ghetko J. Josephine Botsch Laura A. Hehl Dr. Sebastian Kostrhon Dr. Mohit Misra Prof. Dr. Ivan Ðikić Dr. Paul P. Geurink Dr. Hans van Dam Prof. Dr. Brenda A. Schulman Dr. Monique P. C. Mulder 《Angewandte Chemie (International ed. in English)》2023,62(32):e202303319
Protein post-translational modification with ubiquitin (Ub) is a versatile signal regulating almost all aspects of cell biology, and an increasing range of diseases is associated with impaired Ub modification. In this light, the Ub system offers an attractive, yet underexplored route to the development of novel targeted treatments. A promising strategy for small molecule intervention is posed by the final components of the enzymatic ubiquitination cascade, E3 ligases, as they determine the specificity of the protein ubiquitination pathway. Here, we present UbSRhodol, an autoimmolative Ub-based probe, which upon E3 processing liberates the pro-fluorescent dye, amenable to profile the E3 transthiolation activity for recombinant and in cell-extract E3 ligases. UbSRhodol enabled detection of changes in transthiolation efficacy evoked by enzyme key point mutations or conformational changes, and offers an excellent assay reagent amenable to a high-throughput screening setup allowing the identification of small molecules modulating E3 activity. 相似文献
357.
Dr. Penghui Cheng Dr. Rending Wang Dr. Shasha He Dr. Pengpeng Yan Dr. Hongfeng Huang Dr. Jianghua Chen Dr. Jia Shen Prof. Kanyi Pu 《Angewandte Chemie (International ed. in English)》2023,62(39):e202306539
Acute renal allograft rejection (ARAR) after kidney transplantation associated with reduced graft survival and eventual graft failure is poorly diagnosed in hospitals. Here, we report the development of A rtificial bioM arker Pro bes (AMPros) for sensitive urinalysis of ARAR in murine models. AMPros spontaneously go to the kidneys after systemic administration, specifically react with the prodromal immune biomarkers to activate their near-infrared fluorescence signals to report cell-mediated rejection, and efficiently undergo renal excretion into urine. Thus, AMPros enable convenient optical urinalysis that detects ARAR prior to histological manifestation of rejection, which is also earlier than current diagnostic methods measuring proinflammatory cytokines and peripheral blood lymphocyte mRNAs. Due to the high kidney specificity, AMPros-based urinalysis discriminates allograft rejection against other non-alloimmune specific diseases, which is unattainable by measurement of serological biomarkers. Such a noninvasive and sensitive urine test holds great promise in continuous monitoring of renal allograft conditions at low resource settings for timely clinical interventions. 相似文献
358.
Dr. Jeremy Salaam Dr. Thibault Fogeron Dr. Guillaume Pilet Dr. Radu Bolbos Dr. Christophe Bucher Dr. Lhoussain Khrouz Prof. Dr. Jens Hasserodt 《Angewandte Chemie (International ed. in English)》2023,62(7):e202212782
Two mononuclear ferric complexes are reported that respond to a pH change with a 27- and 71-fold jump, respectively, in their capacity to accelerate the longitudinal relaxation rate of water-hydrogen nuclei, and this starting from a negligible base value of only 0.06. This unprecedented performance bodes well for tackling the sensitivity issues hampering the development of Molecular MRI. The two chelates also excel in the fully reversible and fatigue-less nature of this phenomenon. The structural reasons for this performance reside in the macrocyclic nature of the hexa-dentate ligand, as well as the presence of a single pendant arm displaying a five-membered lactam or carbamate which show (perturbed) pKa values of 3.5 in the context of this N6 N5O1 coordination motif. 相似文献
359.
Xiaoxie Ma Yurou Huang Weijie Chen Jia Liu Prof. Sheng Hua Liu Prof. Jun Yin Prof. Guang-Fu Yang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2023,135(3):e202216109
The cationic nature of heptamethine cyanines gives them the capacity to form aggregates with salts by electrostatic interactions. In this work, NaCl promoted J-aggregate formation of aza-coating heptamethine cyanines is explored. NaCl can induce the N-benzyloxycarbonyl Cy-CO2Bz to assemble into a J-aggregate having an absorption at 890 nm. Its excellent fluorescence response to NaCl implies that it has great potential for use as a probe for tracing salt stress in plants. Moreover, NaCl also promotes formation of J-aggregates from the N-ethyloxycarbonyl Cy-CO2Et . The aggregate shows an intense absorption at 910 nm compared to the monomer which absorbs at 766 nm. Its J-aggregated form can serve as a photothermal agent. And the photothermal conversion efficiency is increased from 29.37 % to 57.59 %. This effort leads to the development of two applications of new cyanine J-aggregates including one for tracing salt stress of plants and the other for promoting photothermal therapy of tumors. 相似文献
360.
Dr. Lorena Mendive-Tapia Dr. Laia Miret-Casals Dr. Nicole D. Barth Jinling Wang Dr. Anne de Bray Dr. Massimiliano Beltramo Vincent Robert Prof. Dr. Christophe Ampe Prof. Dr. David J. Hodson Prof. Dr. Annemieke Madder Prof. Dr. Marc Vendrell 《Angewandte Chemie (International ed. in English)》2023,62(20):e202302688
The G protein-coupled kisspeptin receptor (GPR54 or KISS1R) is an important mediator in reproduction, metabolism and cancer biology; however, there are limited fluorescent probes or antibodies for direct imaging of these receptors in cells and intact tissues, which can help to interrogate their multiple biological roles. Herein, we describe the rational design and characterization of a new acid-resistant BODIPY-based amino acid (Trp-BODIPY PLUS), and its implementation for solid-phase synthesis of fluorescent bioactive peptides. Trp-BODIPY PLUS retains the binding capabilities of both short linear and cyclic peptides and displays notable turn-on fluorescence emission upon target binding for wash-free imaging. Finally, we employed Trp-BODIPY PLUS to prepare some of the first fluorogenic kisspeptin-based probes and visualized the expression and localization of GPR54 receptors in human cells and in whole mouse pancreatic islets by fluorescence imaging. 相似文献