Enhancement of ferromagnetic resonance in Al203-doped CozFeAI Heusler alloy film prepared by oblique sputtering

Large and variable in-plane uniaxial magnetic anisotropy in a nanocrystalline （Co2FeA1）97.8（Al2O3）2.2 soft magnetic thin film is obtained by an oblique sputtering method without being induced by magnetic field or post anneaiing. The in-plane uniaxiai magnetic anisotropy varies from 50 Oe to 180 Oe （1 Oe=79.5775 A·m-1） by adjusting the sample＇s position. As a result, the ferromagnetic resonance frequency of the film increases from 1.9 GHz to 3.75 GHz.     

Complexation of Dimethyl 2,3-Naphthalenedicarboxylate with 2-hydroxypropyl-α-, -β- and -γ-cyclodextrins in Aqueous Solution by Fluorescence,Circular Dichroism and Molecular Mechanics

Fluorescence, circular dichroism and molecular mechanics have been used to study the complexation of 2,3-dimethyl naphthalenedicarboxylate with 2-hydroxypropyl-α-, -β and -γ-cyclodextrins (HPCDs) in aqueous solution. Emission spectra upon excitation of the naphthalenedicarboxylate group show two bands whose intensity ratio R is quite sensitive to polarity. From the change of R and lifetimes averages <τ> with HPCD concentration and temperature were obtained the stoichiometry, the association constants and the enthalpy and entropy changes during the complexation. R, <τ> and the fluorescence anisotropies (r) extrapolated at [HPCD]→∞ allows us to estimate the polarity and microviscosity of the media surrounding the guest when complexed. In addition, the analysis of quenching and induced circular dichroism experiments and molecular mechanics calculations in the presence of water, provide information about the forces responsible for the complexation and the geometry of the complexes.     

玄参汤剂的荧光光谱分析

测量了相应的激发光谱以及溶液的pH值,发现同种玄参汤剂中的荧光峰值波长随着激发波长的增加而增加;冷浸玄参汤剂的荧光峰为441 nm,而沸煮玄参汤剂的为532 nm;相应激发光谱由双峰结构向单峰结构转变,并且激发峰值也有明显的红移;同时其pH值由5.5变化到4.1。结合有机物混合体系中的荧光产生机理,解释了上述现象的产生原因：玄参汤剂中的不同荧光分子之间的相互作用以及相同荧光分子内部的能量转移;加热所导致的荧光大分子含量的增加和荧光分子间氢键二聚体的形成。文章对中药玄参的药理学研究具有一定的参考价值,为玄参汤剂质量鉴定提供了一种有效的测量方法。     

Electronic excitation and singlet-triplet coupling in uracil tautomers and uracil-water complexes

Electronic spectra of uracil in its diketo (lactam) form and five enol (lactim) tautomeric forms have been investigated by means of combined density functional and configuration interaction methods. We have simulated the effects of hydrogen bonding with a protic solvent by recomputing the spectrum of uracil in the presence of two, four, or six water molecules. Geometries of the electronic ground state and several low-lying excited states have been optimized. Spin-orbit coupling has been determined for correlated wavefunctions employing a non-empirical spin-orbit mean-field approach. In accord with experiment, we find the diketo tautomer to be the most stable one. The calculations confirm that the first absorption band arises from the ¹( π↦π^*) S ₀↦S ₂ excitation. The experimentally observed vibrational structure in this band originates from a breathing mode of the six ring. Complexation with water molecules is seen to cause a significant blue shift of n↦π^* excitations while leaving π↦π^* excitations nearly uninfluenced. Computed radiative lifetimes are presented for the experimentally known weak phosphorescence from the π↦π^* excited T₁ state. Among the uracil lactim tautomers, one is particularly interesting from a spectroscopic point of view. In this tautomer, the π↦π^* excitation gives rise to the S₁ state. Received 18 February 2002 / Received in final form 5 June 2002 Published online 13 September 2002     

食品色素日落黄与诱惑红荧光特性的研究

应用英国Edinburgh FLS920P型稳态和时间分辨荧光光谱仪,对日落黄与诱惑红溶液进行荧光光谱的实验测量,得到日落黄荧光峰在420 nm,诱惑红荧光峰在432 nm。进一步,应用Gaussian 09W软件构建日落黄分子与诱惑红分子的基态和激发态的几何构型,基于密度泛函理论和含时密度泛函理论,完成对两种分子基态和激发态的优化和计算。优化过程中,采用MPWK泛函和6-31g(d)基组;计算过程中,采用B3LYP泛函和6-311g(d,p)基组。考虑到溶剂效应对计算结果的影响,因此在计算过程中引入了极化连续介质模型,最后计算得到日落黄的荧光峰在435 nm,与实验所得有3.57%的相对误差;计算得到诱惑红荧光峰在443 nm,与实验所得有2.55%的相对误差,两种分子荧光峰的相对误差都在允许范围之内,说明计算合理。在分析光谱特性时,发现两种分子荧光峰的产生与分子内电荷转移有关。     

基于荧光共振能量转移的核酸适体传感器研究

基于荧光共振能量转移的原理,以修饰于核酸适体上的FAM作为能量供体,以氧化石墨烯作为能量受体,构建了荧光适体传感器,分别对不同浓度的胰岛素和多巴胺进行检测.结果表明,胰岛素的线性检测范围为0.05~10μmol/L,多巴胺的线性检测范围为1~500μmol/L,当胰岛素和多巴胺检测浓度相同时,胰岛素检测信号远强于多巴胺.对胰岛素和多巴胺分别进行特异性实验,发现该传感器对胰岛素和多巴胺有较强的特异性.说明基于荧光共振能量转移的核酸适体传感器不仅可实现多种物质的微量检测,还具有较强的选择性,在生物和医药检测领域应用前景广阔.     

氢化物发生-原子荧光光谱法测定海水中的痕量锡

对氢化物发生-原子荧光光谱仪的仪器条件和氢化反应条件进行了研究,建立了测定海水中痕量锡的方法,方法检出限为0.012μg/L,线性范围0.012-20 μg/L,精密度为5.5%.     

Photo-induced Fluorescence of Fluometuron in a Continuous-flow Multicommutation Assembly

This paper deals with the photo-induced fluorimetric determination of the herbicide Fluometuron with the aid of a continuous-flow assembly of the emergent and new methodology known as Multicommutation which was provided with an on-line photoreactor. Maximum fluorescence intensity was observed at basic pH solutions, 1×10⁻⁴ mol l⁻¹ NaOH, after 1.4 min of irradiation and being the maximum at λ_exc 247.0 nm and λ_em 325.0 nm. The influence of different experimental parameters either chemical (pH, surfactants presence, solvent polarity and temperature) or hydrodynamic (time of photo-degradation, size and number of different segments and flow-rate) was tested. The linear dynamic range was from 0.01 to 4.0 mg l⁻¹ of Fluometuron; the inter-day reproducibility (as R.S.D.) of the slope was 0.001% and 1.7% from the peaks intra-day reproducibility. A large series of potential interferents was studied and finally the method was applied to human urine, soil, formulation and water samples.     

Fluorescent Temporin B Derivative and its Binding to Liposomes

Temporins are short (10–13 amino acids) and linear antimicrobial peptides first isolated from the skin of the European red frog, Rana temporaria, and are effective against Gram-positive bacteria and Candida albicans. Similarly to other antimicrobial peptides, the association of temporins to lipid membranes has been concluded to underlie their antimicrobial effects. Accordingly, a detailed understanding of their interactions with phospholipids is needed. We conjugated a fluorophore (Texas Red) to a Cys containing derivative of temporin B (temB) and investigated its binding to liposomes by fluorescence spectroscopy. Circular dichroic spectra for the Cys-mutant recorded in the absence and in the presence of phospholipids were essentially similar to those for temB. A blue shift in the emission spectra and diminished quenching by ferrocyanide (FCN) of Texas Red labeled temporin B (TRC-temB) were seen in the presence of liposomes. Both of these changes can be attributed to the insertion of the Texas Red into the hydrophobic region of the bilayer. Resonance energy transfer, steady state anisotropy, and fluorescence lifetimes further demonstrate the interaction of TRC-temB with liposomes to be enhanced by negatively charged phospholipids. Instead, cholesterol attenuates the association of TRC-temB with membranes. The interactions between TRC-temB and liposomes of varying negative surface charge are driven by electrostatics as well as hydrophobicity. Similarly to native temporin B also TRC-temB forms amyloid type fibers in the presence of negatively charged liposomes. This property is likely to relate to the cytotoxic activity of this peptide.     

三维荧光指纹光谱用于污染河流溶解性有机物来源示踪研究

采用三维荧光指纹光谱技术对河流溶解性有机物荧光特征进行了研究。结果表明,污染河流中的溶解性有机物主要有腐殖质和蛋白质两类,类腐殖质荧光峰λ_激发/λ_发射为250/460 nm(A1),220/400 nm(A2)和325/420 nm(C);类蛋白质荧光峰λ_激发/λ_发射为285/357 nm(T1),230/360 nm(T2)。支流的类蛋白质荧光峰T1和T2由于生活污水的排放,其荧光强度都有明显增强。Fe3+离子在支流与干流汇合后浓度增加到支流的30倍,相应的类腐殖质荧光峰A1也发生了明显蓝移现象,而其他荧光峰则没有明显的偏移。激发波长较长的类腐殖质C,A1和类蛋白质T1荧光强度由于稀释及Fe3+等金属离子猝灭而明显降低,以至荧光峰消失。而较低激发波长的类蛋白质T2和UV类腐殖质A2荧光强度和荧光峰位置相对比较稳定,不容易受到溶液化学条件影响。激发波长220～230 nm荧光团可以用来示踪污染河流溶解性有机物。