毛细管电泳法测定密蒙花中的橙皮素、木犀草素和芹菜素

建立了毛细管电泳法分析密蒙花中橙皮素、木犀草素和芹菜素的方法,研究了缓冲溶液pH和浓度、工作电压和进样时间以及β CD的影响。在优化的条件下,3种物质在15min内得到良好分离。橙皮素、木犀草素和芹菜素的质量浓度分别在0.01～0.50mg mL、0.02～0.70mg mL、0.04～0.80mg mL范围内与峰高成良好线性,线性相关系数分别为0.9992、0.9994和0.9984。3种物质基于迁移时间和峰高的相对标准偏差分别为1 3%、2 2%、2 6%和4 2%、3 7%、4 6%。检出限分别为3 5、4 5和5 0μg mL。     

A novel real-time cell electronic analysis technology for the authentication and quality control of natural medicines

The study was designed to explore the potential applications of the real-time cell electronic analysis technology in the quality evaluation of natural medicines. The natural medicinal Flos Carthami was discussed as a methodological example and the specific time/dose-dependent cell response profiles （TCRPs） were produced by the real-time cell electronic analysis technology. The similarity and bioactivity were obtained by analyzing all TCRPs. Meanwhile, an HPLC method according to the Chinese Pharmacopeia （edition 2010） was used to evaluate the quality of Flos Carthami. The correlation was obtained by comparing the results produced by the two different approaches. By analyzing the data, five different samples ofFlos Carthami can produce remarkably similar TCRPs. The quality ofFlos Carthami was evaluated by both the HPLC and the TCRPs analysis-based approaches and similar results were obtained. The results suggest that the same natural medicine from different locations could produce similar TCRPs. By analyzing the TCRPs, the bioactivity and quality evaluation of natural medicines can be obtained. This technology is a physiologically relevant approach for the quality evaluation of natural medicines. The ultimate aim of our study is to establish a new standard for quality evaluation.     

Simultaneous determination of iridoids, phenolic acids, flavonoids, and saponins in Flos Lonicerae and Flos Lonicerae Japonicae by HPLC-DAD-ELSD coupled with principal component analysis

A method, HPLC coupled with diode-array and evaporative light scattering detectors (HPLC-DAD-ELSD), was newly developed to evaluate the quality of Flos Lonicerae (FL) and Flos Lonicerae Japonicae (FLJ), through a simultaneous determination of multiple types of bioactive components. By employing DAD, the detection wavelengths were set at 240 nm for the determination of iridoids, 330 nm for phenolic acids, and 360 nm for flavonoids, respectively. While ELSD, connected in series after DAD, was applied to the determination of saponins. This assay was fully validated with respect to precision, repeatability, and accuracy. Moreover, principal component analysis (PCA) was used for the similarity evaluation of different samples, and it was proven straightforward and reliable to differentiate FL and FLJ samples from different origins. For PCA, two principal components have been extracted. Principal component 1 (PC1) influences the separation between different sample sets, capturing 54.598% variance, while principal component 2 (PC2) affects differentiation within sample sets, capturing 12.579% variance. In conclusion, simultaneous quantification of bioactive components by HPLC-DAD-ELSD coupled with PCA would be a well-acceptable strategy to differentiate the sources and to comprehensively control the quality of the medicinal plants FL and FLJ.     

高效液相色谱法同时测定金银花中5种有机酸

研究了高效液相色谱法同时测定金银花中5种有机酸(咖啡酸,绿原酸,3,5-O-双咖啡酰基奎宁酸,3,4-O-双咖啡酰基奎宁酸,4,5-O-双咖啡酰基奎宁酸)的方法.金银花样品中5种有效成分用体积分数80%甲醇超声振荡提取,选用Waters Symmetry(R) RP18(3.9 mm i.d.×250 mm,5 μm)色谱柱,甲醇和体积分数0.1%的HAc溶液梯度洗脱,流速为0.8 mL/min,用紫外二极管阵列检测器检测.在该色谱条件下,金银花的5种有效成分在30 min内可达到基线分离.方法的加标回收率为97%～101%,相对标准偏差为0.3%～2.4%.本法适合于同时测定各种金银花中5种有机酸.     

不同产地金莲花中微量元素的测定

采用X射线荧光光谱法(XRF)对3种不同产地的金莲花样品的元素种类和含量进行了测定。结果表明,金莲花中含有K、Ca、Fe、Mg、Zn、Mn等多种微量元素,不同产地金莲花中微量元素种类大致相同,而元素含量却各有差异。这对于鉴别药材的产地特征及金莲花药效功能的进一步研究开发和利用都具有重要意义。     

The Chemical Profiling and Anticancer Potential of Functional Polysaccharides from Flos Sophorae Immaturus

Polysaccharides from Flos Sophorae Immaturus (FSI) are one of its pharmacological compounds that can perform effective activities. Aiming to extract the most effective polysaccharides against hepatocellular carcinoma (HCC), the polysaccharides were separated from FSI through ultrasonic microwave extraction, and the first comparison was carried out on the characterization of the structure and its cytotoxic properties on HCC SMMC 7721 cells of undeproteinized purified polysaccharides (PFSI-1) and papain-deproteinized polysaccharides (PFSI-2) from FSI. The findings indicated that PFSI-1 and PFSI-2 had characteristic absorption peaks of polysaccharides; PFSI-1 contained three monosaccharides and PFSI-2 contained ten; and SEM, AFM, and NMR were consistent with the verification of IR polysaccharide characteristics, suggesting probable additional latent activities. The pharmacotoxic effects of both PFSI-1 and PFSI-2 on SMMC 7721 cells (p < 0.05), attenuated the migration ability of SMMC 7721 cells (p < 0.05) and promoted apoptosis (p < 0.05), with an increase in G0/G1-phase cells and decrease in S-phase cells in the PFSI-1 as well as a decrease in G0/G1-phase cells, increase in S-phase cells, and decrease in apoptosis in the PFSI-2 (p < 0.05). The significant cytotoxic effect of PFSI-2 on SMMC 7721 cells (p < 0.05) and its protective effect on human hepatic L02 cells (HL-7702) at low concentrations (p > 0.05) could indicate its potential as a new drug for the treatment of HCC.     

Simultaneous HPLC Determination of Five Flavonoids in Flos Inulae

A simple and accurate reversed-phase high-performance liquid chromatographic (RP-HPLC) method has been developed for simultaneous determination of five flavonoids, spinacetin, quercetin, luteolin, 6-methoxyluteolin, and isorhamnetin, in an extract of the flowers of Inula britannica L., an important Traditional Chinese Medicine (TCM). Samples were extracted with 80% ethanol. Optimum separation and detection were achieved on an ODS-3 column with a methanol–acetonitrile gradient containing 0.49% (v/v) citric acid as mobile phase. The flow rate was 1.0 mL min⁻¹ and detection was at 360 nm. All calibration plots revealed linearity was good (r ² = 0.999) within the concentration ranges tested. Repeatability was evaluated by performing intra-day and inter-day assays; relative standards deviations (RSD) were less than 2.8%. Recovery of the five flavonoids was between 91.5 and 103.6%, with RSD less than 6.5%. The method was successfully used for analysis of seven samples of Flos Inulae from different parts of China and was found to be simple and efficient.     

CdTe量子点-罗丹明B荧光共振能量转移猝灭法测定金银花中的微量铜

研究CdTe量子点(供体)和罗丹明B(受体)之间荧光共振能量转移的最佳条件,建立了荧光共振能量转移猝灭法测定金银花中微量铜的新方法.采用十六烷基三甲基溴化铵,在pH 6.00的Tris-HCl缓冲液中,Cu2+能对能量转移体系中罗丹明B荧光峰强猝灭从而测定铜的含量.Cu2+浓度在1.3×10-4～3.1×10-2 μg...     

Dynamic microwave-assisted extraction coupled with on-line spectrophotometric determination of safflower yellow in Flos Carthami

A rapid dynamic microwave-assisted extraction and on-line detection by spectrophotometry is proposed for the determination of safflower yellow in Flos Carthami. A high pressure and a peristaltic pump were used to deliver the solvent. A TM₀₁₀ microwave resonance cavity was applied to concentrate the microwave energy and the forward power about 60 W was enough for the extraction. Other extraction conditions also were examined and optimized. In this work, the extraction process can be monitored by measuring the absorption of safflower yellow in the extract, which would be convenient for rapid optimization of the extraction process. The detection and quantification limits are 8 and 27 μg mL⁻¹, respectively. The within-day and between-day precision (R.S.D.) are 1.6-3.2% and 2.8-4.2%, respectively. Compared with off-line detection, the proposed method may provide more rapid measurement and is more convenient for obtaining continuous measurements.     

Analysis of volatile components in herbal pair Semen Persicae‐Flos Carthami by GC‐MS and chemometric resolution

Analysis of volatile components in herbal pair (HP) Semen Persicae‐Flos Carthami (SP‐FC) was performed by GC‐MS coupled with chemometric resolution method (CRM). Furthermore, temperature‐programmed retention indices were used together with mass spectra for identification of the volatile components. With the help of CRM, the two‐dimensional data obtained from GC‐MS instruments were resolved into a pure chromatogram and a mass spectrum of each chemical compound. By use of these methods upon two‐dimensional data, 26, 49, and 59 volatile chemical components in essential oils of single herb Semen Persicae, Flos Carthami, and HP SP‐FC were determined qualitatively and quantitatively, accounting for 78.42, 81.08, and 82.48% total contents of essential oil of single herb Semen Persicae, Flos Carthami, and HP SP‐FC, respectively. It is shown that the accuracy of qualitative and quantitative analysis can be enhanced greatly by means of CRM. It is further demonstrated that the numbers of volatile chemical components of HP SP‐FC are almost the addition of those of two single herbs, but the main volatile chemical components of the former are completely different from those of single herb Semen Persicae or Flos Carthami because of chemical reactions and physical changes occurring in the process of decocting two single herbs. This means that chemical components especially pharmacologically active compounds in the recipe might be different from those of single herbs.