Novel image processing interface to relate DSB spatial distribution from experiments with phosphorylation foci to the state-of-the-art models of DNA breakage

We apply new image processing tools and computer modeling of DSB formation to analyze new experimental data on DSB (DNA double-strand break) yield (#DSB per base pair per Gray). There is LET-dependent DSB clustering within the nucleus volume, and clustering of DSBs along the DNA length, which we show through modeling the whole set of human chromosomes. In recent experiments, DSBs are imaged as phosphorylation sites of the histone protein H2AX, denoted as γH2AX foci, and it is suggested that foci images indicate the spatial distribution of DSBs. For high-LET radiation, DSBs should be located closer to the track center or grouped around ionization sites leading to clustered DSBs as described theoretically, and as can be seen in images. We describe the successful segmentation of foci images and determine foci statistics that will relate models of DSB spatial correlation and clustering along DNA length to the experimental data. The foci data can be used to analyze high-LET effects on DNA fragment sizes and DSB distributions.     

Search for ternary fragmentation in the reaction 856 MeV Mo+V: kinematic probing of intermediate-mass-fragment emissions

A new technique has been applied to coincidence measurements between fission fragments (FF) and intermediate mass fragments (IMF) emitted from the composite system ¹⁴⁹ ₆₅Tb at an excitation energy of 224 MeV. The method permits simultaneous observation of IMF emissions along and normal to the FF separation axes. For the integrated total of 0.10± 0.02 IMF emitted per fission, we find no significant correlation with FF direction, suggesting that IMFs associated with fission reactions are predominantly emitted from the system prior to fission.     

预制破片冲击起爆裸炸药的理论分析

为了研究球形、圆柱形和正多棱柱形预制破片冲击起爆裸炸药的规律,利用Mathcad软件,基于Held高能炸药冲击起爆u2 d判据进行了理论计算与分析,着重比较了破片的质量、密度、形状、长径比、棱数等对冲击起爆裸炸药特性的影响,并与AUTODYN的仿真结果进行了比较。结果表明:对于球形破片,直径一定时,破片密度增大,质量增加,起爆能力增强;相同材料和直径的球形破片与长径比为1的圆柱形破片相比,圆柱形破片的起爆能力优于球形破片。对于圆柱形和正棱柱形破片,密度和质量一定时,随着长径比的增加,破片的起爆能力下降;正棱柱破片的棱数增加时,起爆能力降低,当棱数趋于无穷时,效果趋近于相应的圆柱形破片。     

六胜肽片段的液相合成及组装研究

六胜肽是一种药妆肽,序列为Ac-Glu-Glu-Met-Gln-Arg-Arg-NH2,是相对安全的类肉毒素活性物质.拟用液相方法分别合成片段A[Ac-Glu(Ot Bu)-Glu(Ot Bu)-Met-OH]和片段B[H-Gln(Trt)-Arg-Arg-OH],然后将两个片段在固相载体上进行组装,经切割、纯化得到六胜肽产品,纯度大于98%,片段组装的收率为65%,并对组装反应的条件进行分析和讨论.该方法结合了固相和液相合成的优点,是一种成本低廉、收率较高和周期较短的六胜肽合成方法.     

The Significant Role of Carboxylated Carbonaceous Fragments in the Electrochemistry of Carbon Nanotubes

Carbon nanotubes (CNTs) have been widely employed as electrode materials in diverse branches of electrochemistry, which are claimed to display dramatically improved electrochemical behaviour compared to the conventional carbon materials. But a series of recent publications have demonstrated that the electrocatalysis of CNTs might be due to the presence of some impurities, such as metallic catalysts, nanographitic particles and amorphous carbon. For this reason, CNTs are usually purified or treated with nitric acid or nitric and sulphuric acid prior to their versatile applications. However, the strong acidic and oxidative conditions are so aggressive that serious erosion of the tube structures has inevitably taken place, which creates defects on the sidewalls and gives rise to numerous molecular byproducts, commonly referred as carboxylated carbonaceous fragments (CCFs). The adsorption of CCFs on CNTs greatly alters the surface conditions of CNTs which may significantly impact on their electrochemical properties. To this end, we wish to disclose whether the electrocatalysis of the nitric acid purified CNTs is affected by the adsorption of the CCFs. Ascorbic acid (AA) and β‐nicotinamide adenine dinucleotide (NADH) as selected as the targeting benchmarks that are known to be insensitive to the presence of metallic impurities, which may guarantee the preclusion of the promoting contributions from the metallic catalysts resident in CNTs. We have demonstrated that the electrocatalytic activities of the CNTs are actually dominated by the adsorbed CCFs generated during the acidic pre‐treatment. After removal of the CCFs by base rinse, the electrocatalytic properties of CNTs are greatly deteriorated and degraded to the level similar to the conventional graphite powder. We believe this finding is particularly meaningful to uncover the mysterious electrocatalysis of CNTs.     

Azimuthal distributions of final-state particles and fragments and transverse structure of emission source in high-energy nucleus-nucleus collisions

The azimuthal distributions of final-state particles and fragments produced in high-energy nucleus--nucleus collisions are described by a modified multisource ideal gas model which contains the expansions and movements of the emission sources. The transverse structures of the sources are given in the transverse plane by momentum components p_x and p_y,and described by parameters in the model. The results of the azimuthal distributions, calculated by the Monte Carlo method, are in good agreement with the experimental data in nucleus--nucleus collisions at high energies.     

高速摄影法测量弹丸的破片速度

本文介绍高速摄影法测量弹丸破片飞散速度的几种方法,着重讨论了直接摄影法,它能显示破片在距起爆弹丸较近距离上的防影图象,经数据处理,可获得诸破片速度大小的分布值,还可望求得同一破片在其飞行轨迹上的速度衰减量。     

中能重离子碰撞中的涨落和中间质量碎片发射的机制

在ＱＭＤ模型的基础上，系统研究了４０Ｃａ＋４０Ｃａ中心碰撞系统的中间质量碎片随能量和时间的演化以及它们的动力学起源．集中讨论了反应机制的跃迁，动力学过程中密度涨落和平均中间质量碎片的关系以及碎裂的时间尺度．发现中间质量碎片随能量的上升和下降伴随着涨落的上升和下降．并预言对所研究的反应，当入射能量为６５ＭｅＶ／ｕ时，彻底解体可能发生．碎裂的时间尺度大约为１４０ｆｍ／ｃ，且随能量的增大，此时间尺度不再减小，达到饱和．     

用于爆炸流场与结构间相互作用分析的Euler-Lagrange耦合模拟技术

介绍了1种把Eulerian计算过程和Lagrangian计算过程耦合起来的数值技术,编写了计算程序,把爆炸流场作用过程和结构动力学响应过程的计算结合起来,实现了爆炸流场对结构加载和结构动力响应 及对爆炸流场反作用过程的模拟.利用Eulerian过程的流体弹塑性有限差分计算程序MMIC(multi-materials ...     

高静水压诱导黄瓜稳定遗传变异系的分子分析

 采用高静水压法诱变了黄瓜品种S9925-3,产生了两个稳定的可遗传变异株。采用30对随机配对引物,对该品种的原种及两个变异株进行了扩增片段长度多态性（AFLP）分析。结果表明原种与两个变异植株之间存在明显的多态性。从聚丙烯酰胺凝胶上切下6个差异明显的条带进行DNA测序,并将获得的DNA序列与NCBI（National Center for Biotechnology Information）数据库中的黄瓜DNA数据进行比对分析,确定了变异位点,找到了变异黄瓜系表型变化的可能原因。首次完成了对高静水压处理引起的黄瓜稳定遗传变异系的分子水平分析。研究结果表明,高静水压技术是一种有效的改良作物品系的新诱变工具。