磺硝酚偶氮若丹宁固相萃取光度法测定生物样品中的汞

在 p H2 .0的氯乙酸 -氢氧化钠缓冲介质与吐温 - 80存在下 ,磺硝酚偶氮若丹宁 (NSPAR)与汞反应生成 2∶ 1稳定络合物 ,可被 Waters Sep- Pak C18小柱固相萃取 ,氮 -氮二甲基甲酰氨 (DMF)洗脱后光度法测定 ,λmax=5 5 0 nm,体系摩尔吸光系数ε=8.12× 10 4L· mol-1· cm-1。汞含量在 0 .0 5— 4 .0μg/m L内符合比耳定律 ,用于生物样品中汞含量的测定 ,结果令人满意     

荞麦花多糖的提取及含量测定

采用热水提取、乙醇沉淀的方法从荞麦花中提取出了水溶性多糖,采用硫酸-苯酚法在波长为497nm处进行了糖含量的测定,求得其校准曲线的回归方程为y=0.00508x 0.0294,相关系数为r=0.9978.平均回收率为99%-103%,RSD为0.74%.结果显示:荞麦花多糖的产率为2.5%.糖含量为41.63%.     

萃取—火焰原子吸收法测定骨骼中的微量元素Cu,Zn,Cd和Fe

本文研究了以邻菲罗啉为金属螯合剂，高氯酸钠为配体，在一定酸度下用１，２－二氯乙烷萃取－火焰原子吸收法测定动物骨骼中微量Ｃｕ，Ｚｎ，Ｃｄ和Ｆｅ的条件，并确定了测定方法，方法简单，准确，可靠，元素的检测限分别为：Ｃｕ：０．０３８μｇ／ｍｌ，Ｚｎ：０．００４２μｇ／ｍｌ，Ｃｄ：０．００１９μｇ／ｍｌ，Ｆｅ：０．０２μｇ／ｍｌ，回收率在９２．８％～１０５％之间。     

蒙成药如达多糖的研究

从蒙成药如达中提取水溶性多糖,硫酸-苯酚法测定其含量。平均回收率为99.8%,RSD为0.94%。GC测定了水溶性多糖的组成为葡萄糖、半乳糖和甘露糖。其摩尔比为:6.33:3.25:1.59。     

超临界CO_2萃取板蓝根靛玉红的工艺优化研究

采用正交试验法,对板蓝根靛玉红超临界CO2萃取工艺进行优化。选用萃取温度、萃取压力、萃取时间作为正交试验的3个因素,每个因素选3个水平,以提取物中靛玉红的含量来确定最佳提取条件,并且在最佳条件下确定所加夹带剂的最佳剂量。结果表明最佳工艺为:萃取温度45℃、萃取压力35MPa、萃取时间3h,夹带剂为75%的乙醇200mL。最佳工艺条件下提取靛玉红的得率为4.95μg/g,为传统提取方法的1.6倍。     

紫草的提取工艺

对紫草提取工艺中各参数进行了优化,为紫草工业化提取提供一定的参考;以左旋紫草素含量的测定为工艺指标,通过正交设计试验,对乙醇浓度,乙醇体积,浸泡时间,及渗漉时间各因素进行了考察;紫草最佳提取工艺为：5倍量80%乙醇浸泡16h,再加10倍量80%的乙醇渗漉4h;适宜工艺为：5倍量80%乙醇浸泡12h,再加10倍量80%的乙醇渗漉4h;本工艺设计合理,工艺稳定,左旋紫草素提取率高,质量可控,适宜于工业化生产。     

大蓟多糖提取分离及含量测定

用水提-醇沉法从大蓟中提取多糖,脱脂、去蛋白,经冷冻干燥后得到粗多糖。通过苯酚-硫酸法测定糖的含量,粗多糖得率为6.5%。再采用DEAE-纤维素离子交换柱层析进一步分离纯化,得到5个次级组分DJ1,DJ2,DJ3,DJ4和DJ5。其中得率最高的是DJ2组分,为8.06‰。     

Development of GC–MS/MS method with programmable temperature vaporization large volume injection for monitoring of 17β-estradiol and 2-methoxyestradiol in plasma

Monitoring of estradiol and its metabolites in biological samples is essential for the accurate diagnosis of a number of endocrine diseases. In this study, a sensitive, precise and specific GC–MS/MS method for the quantification of 17β-estradiol (17-BE) and its main metabolite, 2-methoxyestradiol (2-MEOE), in plasma was developed and validated. Plasma concentrations of these steroids are currently investigated as diagnostic markers for pre-eclampsia, a systematic disorder of pregnancy and a leading cause of maternal and fetal morbidity and mortality worldwide.     

Rapid Synergistic Extraction And Atomic Absorption Spectrometric Determination Of Iron In Environmental Samples Using Tributyl Phosphate And N-Phenyl-2-Furylacrylohydoroxamic Acid

Abstract

Iron (III) was rapidly extracted as its ternary complex with tributyl phosphate and N-phenly1-2-furylacrylohydroxamic acid (PFHA) from weakly acidic (pH 1.0-1.5) solutions into isobutyl methyl ketone and was determined atomic absorption spectrometrically. The method tolerated the presences of a large number of anions and cations commonly associated with iron, and was successfully tested with certified samples of coal fly ash, alloys, and plant tissues. It was also applied to the analysis of animal tissues, natural waters, and wastewaters; validating it by standard addition technique. PFHA was selected from nine new hydroxamic acids.     

茉莉花中7种金属元素含量及其浸出率研究

茉莉花是我国的健康饮品,由于茉莉花具有药食两用功效,本实验对茉莉花中7种金属元素含量进行了研究,结果表明,茉莉花中含有丰富的人体所必需的金属矿质元素,K、Ca、Mg的含量较多,具有较高的开发利用价值,但不同金属元素含量存在差异。同时对茉莉花进行了全量浸提,不同元素的浸提率存在一定的差异,其中K的浸出率最高。研究结果对人们日常正确选择饮用花茶提供了一定的理论依据,具有一定的实际意义和推广价值。