Determination of charge states of single swift heavy projectiles by high-energy delta-electrons

The effective charge state is an important particle parameter which is required for the calculation of many effects concerning the interaction between radiation and matter such as an estimate of the radial dose of swift heavy projectiles, stopping power and so on. A new method for the determination of effective charge states of heavy ions is based on the measurement of the number of high-energy delta-electrons which are ejected from a target by the penetrating ion. These electrons are detectable with a CCD-detector and their number can be correlated to the effective charge state of the projectile for known particle velocities. This method is even applicable to operation with single swift heavy ions within statistical bounds.     

Non-minimal pp-wave Einstein–Yang–Mills–Higgs model: color cross-effects induced by curvature

Non-minimal interactions in the pp-wave Einstein–Yang–Mills–Higgs (EYMH) model are shown to give rise to color cross-effects analogous to the magneto-electricity in the Maxwell theory. In order to illustrate the significance of these color cross-effects, we reconstruct the effective (associated, color, and color-acoustic) metrics for the pp-wave non-minimal seven-parameter EYMH model with parallel gauge and scalar background fields. Then these metrics are used as hints for obtaining explicit exact solutions of the non-minimally extended Yang–Mills and Higgs equations for the test fields propagating in the vacuum interacting with curvature. The influence of the non-minimal coupling on the test particle motion is interpreted in terms of the so-called trapped surfaces, introduced in the Analog Gravity theory.     

含有随机夹杂的统计非均匀弹性介质参数的分析

本文提出了随机点场理论用于研究含有随机夹杂的统计非均匀介质。本文不同于其它作者,一般均将随机理论建立在Eshelby的等效夹杂原理之上,而这里是建立在Kunin的微结构理论基础之上。作为理论的一个应用,本文对复合材料的有效模量及夹杂内部及周围微观场进行了计算。     

基于杂交链式反应辅助多重信号放大的端粒酶灵敏检测

端粒酶是真核细胞维持端粒长度的关键逆转录酶,其生物活性的高低可以为多种癌症的临床诊断和预后治疗提供有价值的信息.本研究以人宫颈癌细胞(HeLa细胞)裂解液中的端粒酶为研究对象,通过借助杂交链式反应辅助多重信号放大策略,提出了一种新颖、灵敏的检测端粒酶电化学方法.首先将端粒酶的延伸引物自组装在金电极表面,当端粒酶存在时,端粒酶能够催化引物的延伸,产生与发卡环探针H1部分互补的序列,进而引发杂交链式反应,形成由两个发卡环探针(H1和H2)交替杂交而形成的DNA长链.由于H1和H2末端均修饰有生物素,加入链霉亲和素修饰辣根过氧化物酶后,辣根过氧化物酶被被连接到电极表面,催化邻苯二胺氧化生成2,3-二氨基吩嗪,产生显著的电化学信号.实验结果表明,本研究建立的端粒酶电化学检测方法高效、可行,线性范围宽,灵敏度高,可以检测每毫升10个HeLa细胞裂解液中的端粒酶.本方法具有较好的选择性,能有效区分端粒酶和对照蛋白.     

First- and second-order sensitivities of beams with respect to cross-sectional cracks

This paper deals with a variational formulation for the sensitivity problem of beam systems in the context of deformable solids with cracks. Natural frequencies are defined as state variables involved in the energy functional of the system, while the cracks length and position are treated as system parameters. The hierarchical equation system is formed and solved for the first and second derivatives of the natural frequency functions with respect to the cracks length and position. An analytical procedure for calculations of the second-order sensitivities of natural frequencies is proposed for the non-symmetrical equation system operator. Numerical algorithms are worked out and implemented computationally. Analytical and numerical aspects of the problem are discussed in detail through a number of illustrative results.The support of this work by the State Committee for Scientific Research (KBN) under Grant No. 4-050-0148/17-98-00 is gratefully acknowledged.     

Selection of aptamers for signal transduction proteins by capillary electrophoresis

High‐affinity aptamers for important signal transduction proteins, i.e. Cdc42‐GTP, p21‐activated kinase1 (PAK1) and MRCK (myotonic dystrophy kinase‐related Cdc42‐binding kinase) α were successfully selected in the low micro‐ to nanomolar range using non‐systematic evolution of ligands by exponential enrichment (SELEX) with at least three orders of magnitude enhancement from their respective bulk affinity of naïve DNA library. In the non‐SELEX procedure, CE was used as a highly efficient affinity method to select aptamers for the desired molecular target through a process that involved repetitive steps of partitioning, known as non‐equilibrium CE of equilibrium mixtures with no PCR amplification between successive steps. Various non‐SELEX conditions including the type, concentration and pH of the run buffer were optimized. Other considerations such as salt composition of selection buffer, protein concentration and sample injection size were also studied for high stringency during selection. After identifying the best enriched aptamer pool, randomly selected clones from the aptamer pool were sequenced to obtain the individual DNA sequences. The dissociation constants (K_d) of these sequences were in the low micromolar to nanomolar range, indicating high affinity to the respective proteins. The best binders were also subjected to sequence alignment to generate a phylogenetic tree. No significant consensus region based on approximately 50 sequences for each protein was observed, suggesting the high efficiency of non‐SELEX for the selection of numerous unique sequences with high selectivity.     

Chemometric quality control of chromatographic purity

It is common practice in chromatographic purity analysis of pharmaceutical manufacturing processes to assess the quality of peak integration combined by visual investigation of the chromatogram. This traditional method of visual chromatographic comparison is simple, but is very subjective, laborious and seldom very quantitative. For high-purity drugs it would be particularly difficult to detect the occurrence of an unknown impurity co-eluting with the target compound, which is present in excess compared to any impurity. We hypothesize that this can be achieved through Multivariate Statistical Process Control (MSPC) based on principal component analysis (PCA) modeling. In order to obtain the lowest detection limit, different chromatographic data preprocessing methods such as time alignment, baseline correction and scaling are applied. Historical high performance liquid chromatography (HPLC) chromatograms from a biopharmaceutical in-process analysis are used to build a normal operation condition (NOC) PCA model. Chromatograms added simulated 0.1% impurities with varied resolutions are exposed to the NOC model and monitored with MSPC charts. This study demonstrates that MSPC based on PCA applied on chromatographic purity analysis is a powerful tool for monitoring subtle changes in the chromatographic pattern, providing clear diagnostics of subtly deviating chromatograms. The procedure described in this study can be implemented and operated as the HPLC analysis runs according to the process analytical technology (PAT) concept aiming for real-time release.     

15N216O在1650-3450 cm-1的高分辨红外光谱

采用傅里叶变换红外光谱仪记录了富含¹⁵N₂¹⁶O同位素的一氧化二氮样品在1650-3450 cm^-1波段的高分辨振转光谱,得到了该同位素分子超过7300吸收谱线位置的实验值,经分析实验精确度好于5.0×10^-4 cm^-1. 基于有效哈密顿量模型预测和带带转动分析,确定了所有吸收线的归属;获得了29个新吸收带的振转光谱参数,并优化了其他44个吸收带的光谱参数值. 并且发现有效哈     

无人机光电载荷图像处理器的设计

介绍了无人机载设备的技术特点及机载小型图像处理器的组成。采用TI公司的数字信号处理器芯片为核心处理器,配合现场可编程逻辑器件(FPGA)与外部管理微控制单元(MCU)来实现目标数据的采集与处理,设计了适用于无人机光电载荷的超小型图像处理器系统。针对机载设备的特点,设计时充分考虑了体积、重量、功耗等要求。该系统已应用于多台套机载光电载荷中,工作稳定可靠,满足无人侦察机对捕捉和定位目标的要求。