On-chip fraction collection for multiple selected ssDNA fragments using isolated extraction channels

High efficiency and high-purity fraction collection is highly sought in analysis of fragments-of-interest from selective polymerase chain reaction (PCR) products generated by High Coverage Gene Expression Profiling (HiCEP) methods. Here we demonstrate a new electrophoretic chip device enabling automatic high-efficient fractionation of multiple ssDNA target fragments during a run of separation. We used thoroughly isolated extraction channels for each selected target to reduce the risk of cross-contamination between targets due to cross-talk of extraction channels. Fragments of 35, 108 and 138 b, were successfully isolated, then the recovery was PCR-amplified and assessed by capillary electrophoresis (CE) analysis. Total impurity level of the targets due to unwanted fragments of 0.7%, 2% and 6% respectively, was estimated. Difficulties in collecting multiple target factions are due to band diffusion and DNA adsorption to the walls for the fragments in the separation channel, which is generated by transferring the DNA target fraction from the extraction section to the target reservoir. Therefore, we have carefully measured band broadening and analyzed its influence on the separation resolution due to the delay.     

Sampled-data distributed protocol for coordinated aggregation of multi-agent systems subject to communication delays

This paper studies the coordinated aggregation problem of a multi-agent system. Particularly, all the agents reach a consensus within a pre-specified target region. However, only a subset of agents have access to this target region, and each agent merely interacts with its neighbors by communication. Moreover, there exist unknown heterogeneous delays in communication channels. The underlying communication topology is characterized by a digraph. To accommodate the practical digital disposal, a sampled-data distributed protocol is proposed, where the sampling is asynchronous in the sense that the sampling periods of distinct agents are heterogeneous. The resulting closed-loop system from the proposed sampled-data distributed protocol is in a hybrid fashion that the continuous system is fed-back by using discrete states at sampling instants. The convergence performance of this hybrid closed-loop system is analyzed based on the contraction theory. More specifically, it is first shown that all the states are coordinated to aggregate within the target region, i.e., coordinated aggregation. With this result, it is next shown that all the states are coordinated towards a consensus, i.e., state agreement. These together guarantee the fulfillment of the concerned coordinated aggregation objective. Finally, a simulation example is given to validate the theoretical results.     

基于FT-ICR质谱仪的光解离光谱自动化谱图采集程序的开发与应用

光解离光谱在气相离子结构及动力学研究中发挥着重要的作用。这种方法非常灵活,可在实验室中利用不同种类的激光器及质谱仪的结合来开展相应的研究。但也存在一些问题,例如,自动化水平低,费时,长时间的操作容易引入人为错误,使实验结果的可信度降低。为解决此类问题,该工作开发了一套可用于光谱自动采集和分析的程序AutoMS。该程序包含：(1)AutoSpecMS用于在无需改变原仪器配套的商业化操作软件的前提下,实现多台商业激光器及一台商业高分辨FT-ICR质谱仪的集成,只需要预设参数即可自动获取action光谱;(2)AutoDataMS用于实验数据的分析,可以通过一维、二维或三维图示的方法来展示光解离质谱和光谱等。为验证改程序的可行性,针对tetraphenylpyrrin(四苯基卟啉,TPP),tetra(4-carboxypyryl)porphyrin(四对羧基苯基卟啉,TCPP)以及tetra(4-aminophenyl)porphyrin(四对氨基苯基卟啉,TCPP)三种卟啉衍生物分别开展了相应的实验。使用光解离光谱自动采集程序采集了TPP, TCPP和TAPP在210~700 nm波段的紫外-可见光解离光谱。同时获得了相关光解离碎片离子的单通道光谱,有利于用户对所研究体系的解离机理及解离动力学等方面开展深入的分析探讨。光解离质谱可观察到目标离子在特定波长激光照射下的具体解离碎片,与光解离光谱相结合,可以更好地帮助研究者理解离子在特定波段内的光解离行为。使用AutoDataMS可分别获得样品的2D及3D光解离质谱-光谱,增强了结果的可视化,使研究者更方便地获得光谱中的相关信息。对数据进行进一步分析可看出,不同的取代基对紫外可见光谱的吸收产生了差异明显的影响。此外通过AutoDataMS程序的功能扩展,还实现了对质子化TPP在500～650 nm波段内各离子的相关度分析,加深了对实验数据的理解。由此可见该方法及程序具有较好的扩展性和实用性,并可以为其他相关的工作提供很好的参考。     

Single‐crystal diffraction at the Extreme Conditions beamline P02.2: procedure for collecting and analyzing high‐pressure single‐crystal data

Fast detectors employed at third‐generation synchrotrons have reduced collection times significantly and require the optimization of commercial as well as customized software packages for data reduction and analysis. In this paper a procedure to collect, process and analyze single‐crystal data sets collected at high pressure at the Extreme Conditions beamline (P02.2) at PETRA III, DESY, is presented. A new data image format called `Esperanto' is introduced that is supported by the commercial software package CrysAlis^Pro (Agilent Technologies UK Ltd). The new format acts as a vehicle to transform the most common area‐detector data formats via a translator software. Such a conversion tool has been developed and converts tiff data collected on a Perkin Elmer detector, as well as data collected on a MAR345/555, to be imported into the CrysAlis^Pro software. In order to demonstrate the validity of the new approach, a complete structure refinement of boron‐mullite (Al₅BO₉) collected at a pressure of 19.4 (2) GPa is presented. Details pertaining to the data collections and refinements of B‐mullite are presented.     

22 nm工艺超薄体全耗尽绝缘体上硅晶体管单粒子瞬态效应研究

利用计算机辅助设计技术数值仿真工具, 研究22 nm工艺技术节点下超薄体全耗尽绝缘体上硅晶体管单粒子瞬态效应, 系统地分析了掺杂地平面技术、重离子入射位置、栅功函数和衬底偏置电压对于单粒子瞬态效应的影响. 模拟结果表明, 掺杂地平面和量子效应对于单粒子瞬态效应影响很小, 重离子入射产生大量电荷, 屏蔽了初始电荷分布的差异性. 单粒子瞬态效应以及收集电荷和重离子入射位置强相关, 超薄体全耗尽绝缘体上硅最敏感的区域靠近漏端. 当栅功函数从4.3 eV变化到4.65 eV时, 单粒子瞬态电流峰值从564 μA减小到509 μA, 收集电荷从4.57 fC减小到3.97 fC. 超薄体全耗尽绝缘体上硅器件单粒子瞬态电流峰值被衬底偏置电压强烈调制, 但是收集电荷却与衬底偏置电压弱相关. 关键词： 超薄体全耗尽绝缘体上硅 单粒子瞬态效应 电荷收集 数值仿真     

利用单片机的最小系统改进物理实验

本文根据物理分组和演示实验的要求，利用单片机的最小系统设计并制做了与测温相关的电路，对单片机在物理实验中的开发有一定的参考价值。     

高温超导热图像信号采集和处理系统

本文介绍一种高温超导热图像信号采集和处理系统，采用在ＰＣ机中插入采集板的硬件结构和Ｃ语言与汇编语言混合编程的软件设计方法，实现了对高温超导热图像信号的采集和图像处理，图像管理，图像显示等多种功能。     

First-order definability and algebraicity of the sets of annihilating and generating collections of elements for some relatively free solvable groups

We study the first-order definable, Diophantine, and algebraic subsets in the set of all ordered sets generating a group or generating a group as a normal subgroup for some relatively free solvable groups.     

Bifurcation and stability analysis of a neural network model with distributed delays

In this paper, the dynamics of a generalized two-neuron model with self-connections and distributed delays are investigated, together with the stability of the equilibrium. In particular, the conditions under which the Hopf bifurcation occurs at the equilibrium are obtained for the weak kernel. This means that a family of periodic solutions bifurcates from the equilibrium when the bifurcation parameter exceeds a critical value. Explicit algorithms for determining the stability of the bifurcating periodic solutions and the direction of the Hopf bifurcation are derived by using the theory of normal form and center manifold [20]. Some numerical simulations are given to illustrate the effectiveness of the results found. The obtained results are new and they complement previously known results.This work was supported by the National Natural Science Foundation of China under Grants 60574043 and 60373067, the Natural Science Foundation of Jiangsu Province, China under Grants BK2003053.