An approach to high-speed micro-HPLC

The possibility of high-speed micro-HPLC has been investigated using a short micro-column (35 mm × 0.5 mm i.d.) packed with 3 μm ODS particle. The results indicate that this approach has considerable potential for good separations with short analysis time. A typical example shows only three minutes is required with a plate count of 3,800 to separate seven PAHs.     

Cation-crown ether complex formation in water. II. Alkali and alkaline earth cations and 12-crown-4, 15-crown-5, and 18-crown-6

The stability constants and the partial molal volume and isentropic partial molal compressibility changes of complex formation between cations and crown ethers in water at 25°C are presented. The cations involved are Na⁺, K⁺, Rb⁺, Cs⁺, Ca²⁺, and Ba²⁺, and the crown ethers are 12-crown-4, 15-crown-5, and 18-crown-6. Values of V of complex formation have been discussed in terms of two simple models, one based on the scaled particle theory, and the others on the Drude-Nernst continuum model. The results indicate that the charge of the potassium cation in 18-crown-6 is especially well screened from the water. On this basis hydration numbers of complexed cations have been calculated. This shows that the size of the cation compared to the crown ether hole is important for the contacts between complexed cations and water.     

Sol-Gel Nanometrology: Gated Sampling Can Reveal Initial Sol Formation Kinetics

This report describes a gated sampling approach for studying the initial formation of sol-gel glasses prepared from sodium silicate solution (water glass) and sulphuric acid. Previously described were how changes in particle size and subsequently how sol-gel formation dynamics can be tracked using time-resolved fluorescence anisotropy, by labeling growing silica nanoparticles with suitable fluorescence probes. One limiting factor of this approach was the 2 minute measurement time, which limits this technique for studying the initial sol formation dynamics and limits the measurement precision. Using a continuous flow system and delaying sol flow through different tubing lengths overcomes this problem and allows monitoring of the very early stages of sol formation, second by second after sol preparation, irrespective of the anisotropy measurement time. This technique was applied to studying the initial formation dynamics, within the first 30 seconds, of a 12.01% SiO₂ (w/w), pH 0.66 sol-gel, finding that silica particles of 1.5 nm mean radius are formed within 10 seconds of mixing the sol-gel.     

Direct alpha spectrometry for characterising hot particle properties

Non-destructive analysis methods, especially direct alpha spectrometry but also gamma ray spectrometry and scanning electron microscopy were applied to a nuclear bomb particle from the Thule accident. The use of high-resolution direct alpha spectrometry with the aid of Monte Carlo simulations was demonstrated here in order to determine the properties of this particle. In general, the information that can be obtained through direct alpha spectrometry depends on the information available a priori. For example, known elemental composition makes the assessment of particle dimensions possible even if scanning electron microscopy images are unavailable. Although direct alpha spectrometry may be applied for particle characterisation, further developments in spectrum analysis tools are necessary.     

Freezing Transitions in Non-Fellerian Particle Systems

Non-Fellerian particle systems are characterized by nonlocal interactions, somewhat analogous to non-Gibbsian distributions. They exhibit new phenomena that are unseen in standard interacting particle systems. We consider freezing transitions in one-dimensional non-Fellerian processes which are built from the abelian sandpile additions to which in one case, spin flips are added, and in another case, so called anti-sandpile subtractions. In the first case and as a function of the sandpile addition rate, there is a sharp transition from a non-trivial invariant measure to the trivial invariant measure of the sandpile process. For the combination sandpile plus anti-sandpile, there is a sharp transition from one frozen state to the other anti-state.     

Localization of Non-Relativistic Particles

This paper is a contribution to the problem of particle localization in non-relativistic Quantum Mechanics. Our main results will be (1) to formulate the problem of localization in terms of invariant subspaces of the Hilbert space, and (2) to show that the rigged Hilbert space incorporates particle localization in a natural manner.     

A Discussion on Particle Number and Quantum Indistinguishability

The concept of individuality in quantum mechanics shows radical differences from the concept of individuality in classical physics, as E. Schr?dinger pointed out in the early steps of the theory. Regarding this fact, some authors suggested that quantum mechanics does not possess its own language, and therefore, quantum indistinguishability is not incorporated in the theory from the beginning. Nevertheless, it is possible to represent the idea of quantum indistinguishability with a first-order language using quasiset theory (Q). In this work, we show that Q cannot capture one of the most important features of quantum non-individuality, which is the fact that there are quantum systems for which particle number is not well defined. An axiomatic variant of Q, in which quasicardinal is not a primitive concept (for a kind of quasisets called finite quasisets), is also given. This result encourages the searching of theories in which the quasicardinal, being a secondary concept, stands undefined for some quasisets, besides showing explicitly that in a set theory about collections of truly indistinguishable entities, the quasicardinal needs not necessarily be a primitive concept. Graciela Domenech — Fellow of the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET).     

Uptake of magnetic nanoparticles into cells for cell tracking

A challenge for future applications in nanotechnology is the functional integration of nano-sized materials into cellular structures. Here we investigated superparamagnetic Fe₃O₄ iron oxide nanoparticles coated with a lipid bilayer for uptake into cells and for targeting subcellular compartments. It was found that magnetic nanoparticles (MNPs) are effectively taken up into cells and make cells acquire magnetic activity. Biotin-conjugated MNPs were further functionalized by binding of the fluorescent tag streptavidin–fluorescein isothiocyanate (FITC) and, following uptake into cells, shown to confer magnetic activity and fluorescence labeling. Such FITC-MNPs were localized in the lysosomal compartment of cells which suggests a receptor-mediated uptake mechanism.     

Cadmium colloids from non-aqueous solvents

Cadmium colloids have been prepared by Chemical Liquid Deposition (CLD). The metal is evaporated to yield atoms which are solvated at liquid nitrogen temperature, and upon warming, stable liquid colloids are formed with particle size ranging between 25–100 Å. Zeta potentials were calculated according to the conversion of Hunter and the Hückel equation, for ethanol and dimethyl sulphoxide. UV/VIS measurement of most of the black colloids showed absorption band around 280 nm. For comparison, we prepared CdS colloid with size 400–625 Å. The colloids are stable to oxidation in air and/or oxygen bubbling. The synthesis of colloids and films from Cd with acetone, 2-butanone, ethanol, 2-propanol, 2-methoxyethanol, DMF and DMSO is reported. Transmission Electron Microscopy (TEM) allows us to determine particle size.     

Packing density of slurry-packed capillaries at low aspect ratios

The average interparticle voidage or porosity (epsilon(inter)) in cylindrical capillaries is studied in dependence on the column diameter (d(c)) to particle diameter (d(p)) ratio for 5 < d(c)/d(p) < 50. Using optimized slurry and packing solvents, high pressure and ultrasonication, 5 mum-sized porous C18-silica particles were slurry-packed into fused-silica capillaries having ids from 30 to 250 mum. Packing densities are assessed by a polystyrene standard which is size-excluded from the intraparticle pore space of the packings. For d(c)/d(p) > 35 densely packed beds are realized (epsilon(inter) = 0.36-0.37), while for decreasing aspect ratios an exponential increase in epsilon(inter )is observed reaching epsilon(inter ) approximately 0.47 at d(c)/d(p) = 5. This behaviour is ascribed to a combination of the geometrical wall effect operating in the direct vicinity of the column wall, caused by the inability of the particles to form a dense packing against the hard surface of the column wall, and particle characteristics like the size distribution, shape and surface roughness. Results are compared with the literature data to address also the importance of absolute particle size in studying structure-transport relations in packed beds in dependence on the aspect ratio d(c)/d(p).