溴酸钾氧化中性红催化光度法测定NO-2的研究

研究了盐酸介质中,痕量亚硝酸根催化溴酸钾氧化中性红褪色反应及其动力学条件,建立了催化动力学光度法测定痕量亚硝酸根的新方法,ＮＯ＾－２的测定线性范围为１－７μｇ／２５ｍｌ,检出限为１．５＊１０＾－８ｇ．ｍｌ＾－１。用于化学试剂及唾液中痕量ＮＯ＾－２的测定,结果满意。     

Synthesis and Spectrophotometric Studies of 2-(5-Carboxy-1,3,4-triazolylazo)-5-diethylamino Aniline

In search for new sensitive and selective organic reagents, triazolylazo and imidazolylazo compounds have not been studied until we reported the synthesis and spectrophotometric studies of some reagents in things like that1-2. In this paper, 2-(5-carboxy-1,3,4-triazolylazo)-5-diethylamino aniline (CTZAN) was prepared with the structure as:ExperimentalTo a solution of 3.8 g (0.165 mol) metallic sodium in 30 ml of absolute ethanol, 2-amino-5-carboxy-1,3,4-triazolyl 6.4g (0.050 mol) in 100 ml o…     

醋酸氯己定与甲酚红的荷移反应及应用

研究并确定了醋酸氯已定与甲酚红的荷移反应条件。在乙醇-丙酮介质中形成1：1型络合物,其λmax=455nm,醋酸氯已定浓度在0～80mg/L范围内呈线性关系;表观摩尔吸光系数为1．08×104L·mol-1·cm-1;桑德尔灵敏度是0．0345μg·cm-2;相对标准偏差小于1％（n＝8）,回收率符合要求。     

5-Br-PADAP-NO2--SCN-三元离子缔合物分光光度法测定微量NO2-的研究

研究了在硫酸介质中５－Ｂｒ－ＰＡＤＡＰ与ＮＯ＾－２和ＳＣＮ＾－形成三元离子缔合物的最佳条件,其表观摩尔吸光系数ε５５０＝２．４＊１０＾４Ｌ／（ｍｏｌ．ｃｍ）,缔合物组成比为ｎ（５－Ｂｒ－ＰＡＤＡＰ）：ｎ（ＮＯ＾２）：ｎ（ＳＣＮ＾＿＝１：１：１。     

脑塞通中11种元素含量的测定

采用火焰原子吸收分光光度法测定了脑塞通中钠、钾、镁、钙、铁、铬、锰、镍、镉、铜、锌１１种微量、宏量元素的含量。结果表明,脑塞通中含有丰富的微量,宏观元素。为探讨中成药中微量,宏量与治疗脑血管疾病的关系提供了有用的依据。     

Simultaneous Determination of Nickel(II) and Copper(II) by Second‐Derivative Spectrophotometric Method in Micellar Media

A second‐derivative spectrophotometric method based on zero‐crossing over technique is developed in simultaneous determination of copper(II) and nickel(II) ions. Methylthymol blue (MTB) as a chromogenic reagent and cetyltrimethylammonium bromide as a surfactant were used, and measurements were carried out in buffered solution at pH 6 and at a temperature of 25 °C. The amplitude of derivative spectra was measured at wavelengths of 631.9 and 587.7 nm for the simultaneous determination of Ni²⁺ and Cu²⁺, respectively. Linearity was obtained in the range of 0.5–5.0 μg mL^?1 for both ions in the presence of 0.0–5.0 μg mL^?1 of the other ion as an interfering ion. IUPAC detection limits for Cu²⁺ and Ni²⁺ ions were obtained at 0.48 and 0.43 μg mL^?1, respectively. The proposed procedure has been applied successfully for the simultaneous determination of copper and nickel in synthetic binary mixtures and real samples.     

催化光度法研究血红素受体类过氧化物酶活性

介绍含血红素的过氧化物酶作为超分子受体在仿生催化体系中的应用研究 .以动态分光光度分析法研究血红素蛋白质作为过氧化物酶的催化作用和其在胶束中的类酶活性 .通过对催化过程及反应活性中间体的分子吸收光谱研究 ,发现血红蛋白催化活性与氢供体底物邻苯二胺浓度之间遵从米氏方程 ,并获得牛血红蛋白、血红素及其血红素的环糊精衍生物作为生物催化试剂所具有的类酶特征常数 .     

Fourth derivative spectrophotometric determination of fungicide ziram (zinc(II) dimethyldithiocarbamate) in commercial samples and wheat grains

A procedure has been developed for the direct fourth derivative spectrophotometric determination of zinc(II) dimethyldithiocarbamate by converting it into its copper(II) dimethyldithiocarbamate complex, which is then dissolved in Triton X-100. Beer's law is obeyed over the concentration range 0.5–30?µg/mL in the final solution. Various parameters such as the effect of pH and the interference of a number of ions on the determination of Ziram have been studied in detail. The method is sensitive and can be used for the determination of Ziram in commercial samples like Zirax and Ziron containing Ziram and from wheat grains.     

Spectroscopic methods for determination of catecholamines: A mini-review

Catecholamines are biogenic compounds that perform a variety of vital functions, playing a key physiological role in humans and animals. They are important markers in diagnosis of diseases and dysfunctions as well as widespread components of pharmaceutical formulations. Therefore, development of highly sensitive, rapid, and economically efficient methods for the determination of catecholamines is of great interest. The spectroscopic analytical methods have a good potential in this respect. This mini-review summarizes in a concise manner some advances and trends in the determination of catecholamines by spectroscopic methods, including spectrophotometry, fluorescence spectroscopy, immunoassays with spectroscopic detection, Raman spectroscopy, and other methods. Information on mainly determined compounds and analyzed samples is given and discussed. Analytical strategies and performance for the quantitation of catecholamines in various samples are described.     

Spectrophotometric Determination of Chlorpromazine and Levomepromazine Hydrochlorides In Injectables and Drops By Reaction With Ferric Ion

Abstract

The principal aim of this research was the standardization of a spectrophotometric method using the reaction with ferric ion for quantitative determination of these substances in pharmaceutical preparations. By reaction with ferric ion chlorpromazine gave a pink compound with maximum absorption at 525 nm. the violet product obtained by the reaction with ferric ion and levomepromazine had a maximum at 565 nm. Beer's law was obeyed in a wide range of concentrations for both compounds. the method was applied to simulated and commercially available samples. the efficiency of the method was confirmed by recovery tests.