Modelling and simulation of effect of ultrasonic vibrations on machining of Ti6Al4V

The titanium alloys cause high machining heat generation and consequent rapid wear of cutting tool edges during machining. The ultrasonic assisted turning (UAT) has been found to be very effective in machining of various materials; especially in the machining of “difficult-to-cut” material like Ti6Al4V. The present work is a comprehensive study involving 2D FE transient simulation of UAT in DEFORM framework and their experimental characterization. The simulation shows that UAT reduces the stress level on cutting tool during machining as compared to that of in continuous turning (CT) barring the penetration stage, wherein both tools are subjected to identical stress levels. There is a 40–45% reduction in cutting forces and about 48% reduction in cutting temperature in UAT over that of in CT. However, the reduction magnitude reduces with an increase in the cutting speed. The experimental analysis of UAT process shows that the surface roughness in UAT is lower than in CT, and the UATed surfaces have matte finish as against the glossy finish on the CTed surfaces. Microstructural observations of the chips and machined surfaces in both processes reveal that the intensity of thermal softening and shear band formation is reduced in UAT over that of in CT.     

Microchip electrophoresis of DNA following preconcentration at photopatterned gel membranes

Rapid separation of nucleic acids by microchip electrophoresis could streamline many biological applications, but conventional chip injection strategies offer limited sample stacking, and thus limited sensitivity of detection. We demonstrate the use of photopatterned polyacrylamide membranes in a glass microfluidic device, with or without fixed negative charges, for preconcentration of double-stranded DNA prior to electrophoretic separation to enhance detection limits. We compared performance of the two membrane formulations (neutral or negatively charged) as a function of DNA fragment size, preconcentration time, and preconcentration field strength, with the intent of optimizing preconcentration performance without degrading the subsequent electrophoretic separation. Little size-dependent bias was observed for either membrane formulation when concentrating dsDNA > 100 bp in length, while the negatively charged membrane more effectively blocks passage of single-stranded oligonucleotide DNA (20-mer ssDNA). Baseline resolution of a six-band dye-labeled ladder with fragments 100-2000 bp in size was obtained in <120 s of separation time, with peak efficiencies in the range of 2000-15,000 plates/cm, and detection limits as low as 1 pM per single dye-labeled fragment. The degree of preconcentration is tunable by at least 49-fold, although the efficiency of preconcentration was found to have diminishing returns at high field and/or long times. The neutral membrane was found to be more robust than the negatively charged membrane, with approximately 2.5-fold larger peak area during the subsequent separation, and less decrease in resolution upon increasing the preconcentration field strength.     

3D printed titanium micro-bore columns containing polymer monoliths for reversed-phase liquid chromatography

The potential of 3D selective laser melting (SLM) technology to produce compact, temperature and pressure stable titanium alloy chromatographic columns is explored. A micro bore channel (0.9 mm I.D. × 600 mm long) was produced within a 5 × 30 × 30 mm titanium alloy (Ti–6Al–4V) cuboid, in form of a double handed spiral. A poly(butyl methacrylate-co-ethyleneglycoldimethacrylate) (BuMA-co-EDMA) monolithic stationary phase was thermally polymerised within the channel for application in reversed-phase high-performance liquid chromatography. The prepared monolithic column was applied to the liquid chromatographic separation of intact proteins and peptides. Peak capacities of 69–76 (for 6–8 proteins respectively) were observed during isothermal separation of proteins at 44 °C which were further increased to 73–77 using a thermal step gradient with programmed temperature from 60 °C to 35 °C using an in-house built direct-contact heater/cooler platform based upon matching sized Peltier thermoelectric modules. Rapid temperature gradients were possible due to direct-contact between the planar metal column and the Peltier module, and the high thermal conductivity of the titanium column as compared to a similar stainless steel printed column. The separation of peptides released from a digestion of E.coli was also achieved in less than 35 min with ca. 40 distinguishable peaks at 210 nm.     

数字图像相关方法在板载芯片封装热变形测量中的应用

针对板载芯片封装结构中由于各层材料热膨胀系数的差异引起的热失配现象,利用数字图像相关方法对板载芯片封装结构在热载荷下的表面热变形分布进行实验测量,并比较了不同封装配置对结构热变形的影响.建立了适于求解结构表面热变形分布的理论模型,利用实验结果和有限元模拟验证了理论模型.同时表明了实验方法的有效性和可行性,为微机电系统器件设计提供了有益的参考.
关键词:       测量|热变形|数字图像相关方法|板载芯片封装|微机电系统     

用于微流控液滴检测的共焦激光诱导荧光检测系统

以发射波长为473nm的二极管泵浦激光器为激发光源,搭建适于微流控液滴检测的共聚焦激光诱导荧光检测系统.系统玻璃芯片采用简易加工技术加工而成.并用2%十八烷基三氯硅烷将微通道处理为具有强疏水性质.荧光素钠溶液在T形通道交叉处被十四烷剪切形成液滴,液滴流经检测点时被激光诱导荧光系统检测.荧光素钠的检出限为1.1×10<'...     

Chip‐based capillary electrophoresis profiling of olive pollen extracts used for allergy diagnosis and immunotherapy

Standardization of protein extracts for clinical purposes represents an important task in order to maintain adequate reactivity, presence of the relevant allergens, and safety among other factors. The main objective of this work was to explore the potential use of a chip‐based automated CE system commercially available to analyze several of the most common forms of allergenic extracts from olive pollen used in allergy clinics. These include experimental extracts prepared from olive pollens, in‐house reference extracts, extracts designed for skin prick test assays, and a panel of vaccine variants aimed to specific immunotherapy. As a major conclusion of the study, chip‐based CE allowed in all cases to determine accurate protein profiles with different degrees of sensitivity, where several allergens (particularly the major olive pollen allergen Ole e 1) were easily recognized. Moreover, several purified allergens were also analyzed by this method, and proposed as specific standards for different purposes. In the present condition, the method can only provide the protein profile of the extracts with respect to a preestablished standard extract, but not allergen identification. However, these and other future developments and applications are discussed.     

微流体技术在电子芯片冷却中的应用研究进展

随着微电子技术向小型化集成化及高频高速方向发展,计算机芯片集成度的提高受到因电子元器件发热而引起的热障所限制,芯片冷却问题成为影响计算机进一步发展的关键因素之一。介绍了电子芯片发展的现状及主要冷却方法的发展,从冷却驱动器件、微通道结构各个角度论述了微流体技术在电子芯片冷却中的重要作用,重点介绍了微槽道冷却和微喷冷却的微流体技术特征,论述了压电泵、电渗、热管等微流体驱动技术在冷却液驱动的应用。     

斯特林制冷机测试系统设计

介绍了一种斯特林制冷机测试系统的设计。该测试系统可检测制冷机系统各参数,包括斯特林制冷机驱动控制器输入电压、电流,制冷机冷头温度、输出电压、电流、频率等,对制冷机系统的工作状态进行分析和判断,并将所测试的结果以数字和图表方式实时显示。当制冷机系统出现故障时及时判断并显示故障类别,同时可以通过触摸显示屏设置制冷机系统各工作参数。