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71.
72.
高效液相色谱切换波长法测定油菜内源激素 总被引:1,自引:0,他引:1
采用反相高效液相色谱法(HPLC)切换波长法同时测定油菜中的内源激素赤霉素(GA3)、3-吲哚乙酸(IAA)和脱落酸(ABA),以甲醇:水:乙腈(20:60:20)为流动相,结果显示其检出限分别为GA3:0.08mg/L;IAA:0.015mg/L;ABA:0.025mg/L,比单波长检测的检出限提高了4-10倍;相对标准偏差(RSD)分别为GA3:6.3%,IAA:4.34%,ABA:7.95%.本实验采用切换波长法,显著减低了各组分的检出限,建立了一种以高灵敏度对多组分体系同时进行检测的方法.同时为快速、准确测定油菜内源激素提供了可靠方法. 相似文献
73.
74.
采用Plackett-Buman(P-B)法和中心复合设计(Central Composite Design,简称CCD)对影响固相萃取安定的6个因素进行筛选优化。P-B实验设计与统计学分析表明:pH、上样速度、洗脱液用量是影响回收率的3个关键因素。以回收率为响应目标,对3因素进行中心复合设计,并经响应面法优化分析得到影响回收率的二阶模型,确定了安定萃取实验的最优操作条件:pH10.20,上样速度0.67 mL/min,洗脱液用量2.60 mL,实测回收率达到91.26%。在0.10~10.00μg/mL的范围内本方法线性良好(R2>0.99),检测限为0.07μg/mL,日内和日间相对标准偏差(RSD)<10%,准确度(RE)<±6.0%。 相似文献
75.
Upper bound limit analysis of active earth pressure with different fracture surface and nonlinear yield criterion 总被引:7,自引:0,他引:7
Conventional calculations of static and seismic active earth pressures of soils on a retaining wall are formulated assuming the soils obeying a linear Mohr–Coulomb yield criterion. However, experimental evidences show that the strength envelopes of almost all geomaterials are nonlinear in nature over a wide range of normal stresses. In this paper, the strength envelope of the backfill behind a retaining wall is considered to follow a nonlinear yield criterion. A simple method is proposed for calculating the static and seismic active earth pressures acting against a retaining wall using a nonlinear yield criterion. This method is based on the upper bound theorem of limit analysis. Both translational and rotational fracture surfaces are employed in the formulation for calculating active earth pressures. Quasi-static representation of earthquake effects using a seismic coefficient concept is adopted for seismic active earth pressure calculations. Instead of using directly the actual nonlinear yield criterion, a linear Mohr–Coulomb yield criterion, which is tangential to the nonlinear yield criterion, is used to formulate the active earth pressure problem as a classical nonlinear programming problem. A nonlinear sequential quadratic programming algorithm is used to search for the maximum solution. In order to assess the validity of the proposed method, values of active earth pressures for different values of seismic coefficients and nonlinear parameters in the yield criterion are calculated and compared with solutions obtained using an extended Rankine’s active earth pressure theory. For the case of static active earth pressure, the upper bound solutions using the present method with a translational fracture surface are equal to the extended Rankine’s theoretical solutions and are slightly smaller than those obtained using the present method with a rotational fracture surface. For the case of seismic active earth pressure, numerical results obtained using the present method with a rotational fracture surface is very close to the extended Rankine’s theoretical solutions. A study is conducted to investigate the effects of the parameters in the nonlinear yield criterion on the active earth pressures. 相似文献
76.
By means of the jet-bundle formalism, the Second Noether Theorem is formulated for a general first-order Lagrangian field theory with infinitesimal local symmetries. These symmetries are implemented by a linear differential operator acting between the sections of a vector bundle and vector fields on the configuration bundle. The problem of the degeneration of the Lagrangian system is examined from a covariant and an instantaneous (i.e. space+time split) viewpoint. It is shown that in the instantaneous approach the presence of infinitesimal local symmetries leads to degeneration of the theory. Vertical local symmetries are shown to imply degeneration also in the covariant formalism. These results can be extended to higher-order Lagrangians as well. 相似文献
77.
In this paper, we study the explicit expansion of the first order Melnikov function near a double homoclinic loop passing through a nilpotent saddle of order m in a near-Hamiltonian system. For any positive integer , we derive the formulas of the coefficients in the expansion, which can be used to study the limit cycle bifurcations for near-Hamiltonian systems. In particular, for , we use the coefficients to consider the limit cycle bifurcations of general near-Hamiltonian systems and give the existence conditions for 10, 11, 13, 15 and 16 (11, 13 and 16, respectively) limit cycles in the case that the homoclinic loop is of cuspidal type (smooth type, respectively) and their distributions. As an application, we consider a near-Hamiltonian system with a nilpotent saddle of order 2 and obtain the lower bounds of the maximal number of limit cycles. 相似文献
78.
79.
In this paper, we deal with the critical problems in residue arithmetic. The reverse conversion from a Residue Number System (RNS) to positional notation is a main non-modular operation, and it constitutes a basis of other non-modular procedures used to implement various computational algorithms. We present a novel approach to the parallel reverse conversion from the residue code into a weighted number representation in the Mixed-Radix System (MRS). In our proposed method, the calculation of mixed-radix digits reduces to a parallel summation of the small word-length residues in the independent modular channels corresponding to the primary RNS moduli. The computational complexity of the developed method concerning both required modular addition operations and one-input lookup tables is estimated as , where k equals the number of used moduli. The time complexity is modular clock cycles. In pipeline mode, the throughput rate of the proposed algorithm is one reverse conversion in one modular clock cycle. 相似文献
80.
Zhifu Xie 《Physics letters. A》2011,375(39):3392-3398
This Letter is to provide a new approach to study the phenomena of degeneracy of the number of the collinear central configurations under geometric equivalence. A direct and simple explicit parametric expression of the singular surface H3 is constructed in the mass space (m1,m2,m3)∈3(R+). The construction of H3 is from an inverse respective, that is, by specifying positions for the bodies and then determining the masses that are possible to yield a central configuration. It reveals the relationship between the phenomena of degeneracy and the inverse problem of central configurations. We prove that the number of central configurations is decreased to 3!/2−1=2, m1, m2, and m3 are mutually distinct if m∈H3. Moreover, we know not only the number of central configurations but also what the nonequivalent central configurations are. 相似文献