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31.
《Electroanalysis》2006,18(24):2451-2457
This paper describes a layer‐by‐layer (LBL) self‐assembly process of chitosan (CTS) and gold nanoparticles (Au) on the pretreated negatively charged glassy carbon (GC) electrode to fabricate electrochemistry immunosensor with a nontoxic biomimetic interface, which provided an environment similar to a native system and allowed more freedom in orientation for immobilization of carcinoembryonic antibody (anti‐CEA) to monitor carcinoembryonic antigen (CEA). UV‐vis spectroscope, atomic force microscopy (AFM), and cyclic voltammetric (CV) measurements were used to follow the multilayer film formation. The performance of the biominetic interface and factors influencing the assay system were investigated in detail. The differential pulse voltammetry (DPV) current response is used for the CEA concentration assay. The dynamic range was from 0.50 to 80.00 ng mL?1 with a detection limit of 0.27 ng mL?1 at 3σ. In addition, the experiment results indicate that immobilization described in this proposed method exhibits a good sensitivity, selectivity, and stability.  相似文献   
32.
In the present paper, we simulate focused wave fields generated by a system of plane transducers used in an immersion ultrasonic technique. The ultrasonic beam penetrates into the elastic medium through the fluid-solid boundary. A real-time computer algorithm is proposed to calculate stress components in the solid medium. It is shown that a good focusing wave structure can be provided by a pair of plane rectangular transducers, inclined with respect to each other and to the beam acoustic axis.  相似文献   
33.
An upper bound for the number of components of the numerical range of matrix polynomials is established. We also establish a necessary condition and a sufficient condition for the connectedness of the numerical range of a quadratic selfadjoint matrix polynomial. The boundary of the numerical range of linear matrix polynomials is also considered.  相似文献   
34.
This paper presents detailed information on the experience gained during the attempts to model a set of transitional flows due to boundary layer separation. These flows are developed on a flat plate with a semi-circular leading edge and they have been coded by the ERCOFTAC Special Interest Group on Transition, as T3L flows. Different freestream velocities and turbulence intensities configure these transitional flows and, by consequence, govern the transition mechanism, resulting in larger or smaller transitional regions. The modeling of the T3L flows has been performed by adopting a low-Reynolds number second-moment closure turbulence model. The results showed satisfactory agreement with the experimental measurements, although some difficulties regarding successful convergence have been faced. The final conclusion is that turbulence modeling can present quite accurate results for transitional flows without any additional use of ad-hoc modifications or additional equations based on various transition models and intermittency transport modeling.  相似文献   
35.
The method of equivalent variational methods, originally due to Carathéodory for free problems in the calculus of variations is extended to investigate boundary value problems for a class of second order differential equations on the half-line. Some applications are presented to illustrate the potential of this method.  相似文献   
36.
This paper is concerned with the spectral analysis of a one-velocity transport operator with Maxwell boundary condition in L 1-space. After a detailed spectral analysis it is shown that the associated Cauchy problem is governed by a C 0-semigroup. Next, we discuss the irreducibility of the transport semigroup. In particular, we show that the transport semigroup is irreducible. Finally, a spectral decomposition of the solutions into an asymptotic term and a transient one which will be estimated for smooth initial data is given.  相似文献   
37.
四阶微分方程三点边值问题的奇摄动   总被引:6,自引:0,他引:6       下载免费PDF全文
该文用Shauder不动点定理及微分不等式理论研究了一类非线性四阶常微分方程三点边值问题的存在性,并利用边界层校正法获得解及其二阶导数的高阶渐进展开式.  相似文献   
38.
New existence and uniqueness proofs are given for the solutions of the equations governing the self-similar compressible boundary layer (Falkner-Skan equations). The properties of the solutions are studied and some bounds on important quantities are concluded. The paper is restricted to favourable pressure gradients and to wall cooling.  相似文献   
39.
Let Au(BG) be the Banach algebra of all complex valued functions defined on the closed unit ball BG of a complex Banach space G which are uniformly continuous on BG and holomorphic in the interior of BG, endowed with the sup norm. A characterization of the boundaries for Au(BG) is given in case G belongs to a class of Banach spaces that includes the pre-dual of a Lorentz sequence space studied by Gowers in Israel J. Math. 69 (1990) 129-151. The non-existence of the Shilov boundary for Au(BG) is also proved.  相似文献   
40.
Summary A 2D time-domain Boundary Element Method (BEM) is applied to solve the problem of transient scattering of plane waves by an inclusion with a unilateral smooth contact interface. The incident wave is assumed strong enough so that localized separations take place along the interface. The present problem is indeed a nonlinear boundary value problem since the mixed boundary conditions involve unknown intervals (separation and contact regions). In order to determine the unknown intervals, an iterative technique is developed. As an example, we consider the scattering of plane waves by the cross section of a circular cylinder embedded in an infinite solid. Numerical results for the near field solutions are presented. The distortion of the response waves and the variation of the interface states are discussed. The financial support by the China National Natural Science Foundation under Grant No. 19872001 and No. 59878004 is gratefully acknowledged. The second author is also grateful to the support of the National Science Fund for Distinguished Young Scholars under Grant No. 10025211.  相似文献   
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