一类拟线性Schroedinger方程

在二维空间中讨论一类拟线性Schroedinger方程，该方程在物理学上描述了吸引玻色-爱因斯坦凝聚．通过建立这个方程的性质，运用能量方法，证明了该方程所对应的初值问题的解在一定条件下爆破．同时利用变分方法，也得到了整体解存在的一个充分条件，该条件与一个经典的椭圆方程的基态有关．     

A STUDY ON GRADIENT BLOW UP FOR VISCOSITY SOLUTIONS OF FULLY NONLINEAR, UNIFORMLY ELLIPTIC EQUATIONS

We investigate sharp conditions for boundary and interior gradient estimates of continuous viscosity solutions to fully nonlinear,uniformly elliptic equations under Dirichlet boundary conditions. When ...     

Conformational analysis of a secondary hydroxamic acid in aqueous solution by NOE spectroscopy

Hydroxamic acids are metal‐binding compounds used by micro‐organisms and possess applications in medicine and industry. Hydroxamic acids favor two conformations, E and Z; metal binding is limited to the Z conformation. The Z conformation may be identifiable by NOE spectroscopy, but analysis is complicated by the potential for long‐range coupling as well as for relayed NOEs due to conformational switching. In this report, we re‐examine the reported conformational preference of N‐methyl acetohydroxamic acid (NMHA) in D₂O using NOE spectroscopy. We find that the favored conformation of NMHA in aqueous solution is the E conformation, contrary to an earlier report. NOE build‐up curves are proposed as a valuable tool to probe conformational behavior in similar systems. Copyright © 2013 John Wiley & Sons, Ltd.     

Determination of atorvastatin in human serum by salting out assisted solvent extraction and reversed-phase high-performance liquid chromatography–UV detection

A simple and rapid technique based on salting out assisted solvent extraction was developed for extraction of atorvastatin from serum sample and high performance liquid chromatography–UV was used for its detection. In the present study, 1.0 mL serum was extracted by 0.5 mL of acetonitrile and some parameters that can affect extraction such as type and volume of extraction solvent, type of salt, and pH were optimized. Under optimized experimental conditions, the calibration curve was found to be linear in the range of 0.001–10 ng mL⁻¹ in human serum and the correlation coefficient (R²) and the limits of detection were >0.99 and 0.0005 ng mL⁻¹, respectively. The accuracy of the method in terms of average recovery of the compound in spiked serum and water samples was better than 90%.     

Infrared diode laser spectroscopy of the ν = 2 band of AlF

A vibrational–rotational spectrum of the ν = 2 transitions of a high-temperature molecule AlF was observed between 1490 and 1586 cm⁻¹ with a diode laser spectrometer. Measurements were made on the ν = 3–1, 4–2, 5–3 and 8–6 bands at a temperature of 900 °C. Measured spectral lines were fitted to effective band constants ν₀, B_ν and D_ν for each band. Present measurements were made with only one Pb-salt laser diode. Physical significance of the effective band constants is discussed.     

Simultaneous determination of anthracyclines and taxanes in human serum using online sample extraction coupled to high performance liquid chromatography with UV detection

An online SPE‐LC method that can determine both anthracyclines and taxanes simultaneously in human serum samples is reported. The entire method of extraction, separation and UV detection was achieved online by column switching between an SPE column (Biotrap 500 (20×4 mm)) and an analytical column (Zorbax XDB C18, 150×4.6 mm, 5 μm) with a 23 min total cycle time. The method is linear (r²>0.998) over the range of 0.5–25 μg/mL. The analytes of interest are retained on the SPE column with good recovery (84–117%), while proteins and other serum components elute to waste. This online clean‐up is much faster (150 s) and less manual than traditional off‐line extraction methods. Using 0.1 mL spiked serum samples, the LOQ was 0.5 μg/mL. Intra‐ and inter‐day precision were acceptable (≤15% RSD) at and above the LOQ. The method was applied to the analysis of serum samples from patients undergoing chemotherapy with these agents.     

Sol-gel immunoaffinity chromatography for the clean up of ochratoxin A contaminated grains

This paper describes the application of sol-gel immunoaffinity columns for clean up of ochratoxin A contaminated cereal crops. Monoclonal antibodies selective for OTA have been entrapped into the pores of a sol-gel matrix in order to prepare immunoaffinity columns. Different parameters such as amount of entrapped antibodies and loading conditions were optimized to obtain highest possible recoveries of OTA. The method has been found to be a suitable tool in sample preparation prior to HPLC-FLD determination and as selective as conventional commercially available immunoaffinity columns. In the clean up of different cereals mean recoveries of 82±5%, 90±6% and 91±3%, were obtained for wheat, barley and rye, respectively, with sol-gel columns containing 1mg of anti-OTA antibodies. The detection limit (signal-to-noise ratio, 3) was 0.5 μg/kg and the limit of quantification (signal-to-noise ratio, 10) determined to be 1 μg/kg. Sol-gel columns can be reused 7 times without significant loss of recovery. After 10 applications the recovery decreased to approx. 50%.     

低电压扫描电子显微术

低电压扫描电子显微术是研究半导体、绝缘体和生物材料的有效方法。本文综述了这种方法的优点、限制和关于扫描电镜的改进,并且提供了实际操作的某些要领。     

液相色谱在线净化-电喷雾串联质谱测定水产品中大环内酯和林可胺类药物残留

建立了水产品中大环内酯类抗生素[红霉素(ERM)、罗红霉素(ROM)、替米卡星(TIL)、泰乐菌素(TYL)、北里霉素(KIT)、交沙霉素(JOS)、竹桃霉素(OLM)、螺旋霉素Ⅰ(SPM-Ⅰ)]和林可胺类(林可霉素(LIN)和氯林可霉素(CLD))的高效液相色谱-电喷雾串联质谱(LC-ESI-MS/MS)检测方法.样品经提取、反相液相色谱分离净化后进行质谱分析,在选择反应监测模式(SRM)下进行特征母-子离子对信号采集.根据保留时间和母离子及两个特征子离子信息定性分析,以基峰离子进行定量.大环内酯类残留的检出限(S/N=3)为0.1～0.2μg/kg,定量限为1.0μg/kg,在1.0～200 ng/mL时峰强度与质量浓度的线性关系良好(R~2 >0.99).在虾、鳗鱼和带鱼3种基质中1.0、2.0、10.0μg/kg 3个添加水平下,除个别药物外,药物的平均回收率范围为64%～114%,RSD<12%.该法适用于各种水产品中大环内酯类残留的分析.