高效液相色谱法鉴别黑色签字笔墨水字迹形成时间的研究

签字笔携带方便、书写流畅,现已成为常用的书写工具之一。在许多刑事及民事案件中,尤其在呈明显上升趋势的各类贪污、受贿案件中,经常会遇到由签字笔墨水形成的契约、合同、收据、借条等可疑文件中关于签字笔墨水字迹色痕的鉴定。对于字迹色痕形成时间的鉴定工作一直是困扰法庭     

Rapid and sensitive determination of protein by light-scattering technique with Eriochrome Blue Black R

Based on the interaction between Eriochrome Blue Black R (EBBR) and proteins, which causes a strong light-scattering signal with the maximum scattering peak located at 398 nm, a simple, rapid, sensitive and selective method is developed for the determination of proteins by the light-scattering technique using a common spectrofluoremeter. Under proper experimental conditions, the protein determination can be performed in the range of 0.1-25, 0.1-20 and 0.25-25 μg ml⁻¹ for bovine serum albumin (BSA), human serum albumin (HSA) and human immunoglobulin G (IgG), respectively. The detection limit, calculated as 3 times the S.D. of nine blank measurements, are 33 μg l⁻¹ for BSA, 25 μg l⁻¹ for HSA and 38 μg l⁻¹ for IgG. Moreover, there is no significant difference among the scattering signals yielded by HSA, IgG and BSA, and almost no interference of many amino acids and metal ions. The method has been satisfactorily applied to the direct determination of the total protein in human serum, saliva and urine samples. The results obtained from the studies on the binding characteristics of EBBR to BSA indicated that an electrostatic force existed in the binding system, and the binding constant (K) and the number of the binding sites (n) at 25 °C are 1.69×10⁵ l mol⁻¹ and 0.946, respectively.     

Mechanisms of oxidation of guanine in DNA by carbonate radical anion, a decomposition product of nitrosoperoxycarbonate

Peroxynitrite is produced during inflammation and combines rapidly with carbon dioxide to yield the unstable nitrosoperoxycarbonate, which decomposes (in part) to CO(3) (.-) and (.)NO(2) radicals. The CO(3) (.-) radicals oxidize guanine bases in DNA through a one-electron transfer reaction process that ultimately results in the formation of stable guanine oxidation products. Here we have explored these mechanisms, starting with a spectroscopic study of the kinetics of electron transfer from 20-22mer double-stranded oligonucleotides to CO(3) (.-) radicals, together with the effects of base sequence on the formation of the end-products in runs of one, two, or three contiguous guanines. The distributions of these alkali-labile lesions were determined by gel electrophoresis methods. The cascade of events was initiated through the use of 308 nm XeCl excimer laser pulses to generate CO(3) (.-) radicals by an established method based on the photodissociation of persulfate to sulfate radicals and the oxidation of bicarbonate. Although the Saito model (Saito et al., J. Am. Chem. Soc. 1995, 117, 6406-6407) predicts relative ease of one-electron oxidations in DNA, following the trend 5'-GGG > 5'-GG > 5'-G, we found that the rate constants for CO(3) (.-)-mediated oxidation of guanines in these sequence contexts (k(5)) showed only small variation within a narrow range [(1.5-3.0)x10(7) M(-1) s(-1)]. In contrast, the distributions of the end-products are dependent on the base sequence context and are higher at the 5'-G in 5'-GG sequences and at the first two 5'-guanines in the 5'-GGG sequences. These effects are attributed to a combination of initial hole distributions among the contiguous guanines and the subsequent differences in chemical reaction yields at each guanine. The lack of dependence of k(5) on sequence context indicates that the one-electron oxidation of guanine in DNA by CO(3) (.-) radicals occurs by an inner-sphere mechanism.     

对Reissner-Nordstrm-anti-de Sitter黑洞Hawking隧穿辐射的研究

在考虑粒子间的自引力相互作用后,首先采用Parikh-Wilczek的半经典隧穿方法对带电粒子与不带电粒子在Reissner-Nordstrm-anti-de Sitter黑洞视界处的隧穿辐射特征进行了研究.其次,推广复路径方法对该黑洞的辐射谱进行研究.两种方法所得的结果都支持Parikh-Wilczek的观点,即黑洞的隧穿率Bekenstein-Hawking熵变有关,真实的辐射谱不是纯热谱.     

Characteristics analysis of extruded elliptical hole photonic crystal fibers with square air-core

An extruded elliptical hole photonic crystal fibers PCF with square air-core is proposed. By using a full vector finite-element method FV-FEM and anisotropic perfectly matched layers APML, the structure and optical properties of the proposed PCF are analyzed. Simulation results show that the birefringence of the proposed photonic crystal fiber can be up to the order of 10⁻², and has a flattened dispersion from 1.20 μm to 1.80 μm. The proposed PCF may have important application in super-continuum SC generation, dispersion compensation, fiber-optic sensing systems and other aspects.     

Tunneling of Charged and Magnetized Fermions from the Kerr-Newman-Ads Black Hole with Magnetic Charges

Tunneling of charged and magnetized Dirac particles from the Kerr-Newman-Ads black hole with magnetic charges is discussed in this paper. Owing to the electric and magnetic fields would couple with gravity field, we introduce the Dirac equation of charged and magnetized particles. Then by redefining the equivalent charge and gauge potential corresponding to the source with electric and magnetic charges, we discuss this tunneling once and obtain the same Hawking temperature. Both results show that the fermions tunneling formalism also come into existence in the charged and magnetized background space time.     

Schwarzschild Radius Before General Relativity: Why Does Michell-Laplace Argument Provide the Correct Answer?

A famous Newtonian argument by Michell and Laplace, regarding the existence of “dark bodies” and dating back to the end of the 18th century, is able to provide an exact general-relativistic result, namely the exact formula for the Schwarzschild radius. Since general relativity was formulated more than a century after this argument had been issued, it looks quite surprising that such a correct prediction could have been possible. Far from being merely a fortuitous coincidence (as one might justifiably be induced to think), this fact can find a reasonable explanation once the question is approached the other way round, i.e. from the general-relativistic point of view. By reexamining Laplace’s proof from this point of view, we discuss here the reasons why Michell-Laplace argument can be so “unexpectedly" correct in its general-relativistic prediction.     

Fermions tunneling from the Horowitz-Strominger Dilaton black hole

Based on the work of Kerner and Mann, fermions tunneling from the Horowitz-Strominger Dilaton black hole on the membrane is studied. Owing to the coupling among electromagnetic field, matter field and gravity field, the Dirac equation of charged particles is introduced, and according to that, the expected emission temperature is obtained. After the self-gravitational interaction is considered, it is found that the tunneling rate of fermions also satisfies the underlying Unitary theory as the case of scalar particles. Supported by the Natural Science Foundation of Sichuan Education Office (Grant No. 07ZC039)     

Transverse Wave Propagation in Relativistic Two-Fluid Plasmas around Schwarzschild-de Sitter Black Hole

We investigate transverse electromagnetic waves propagating in a plasma influenced by the gravitational field of the Schwarzschild-de Sitter black hole. Applying 3+1 spacetime split we derive the relativistic two-fluid equations to take account of gravitational effects due to the event horizon and describe the set of simultaneous linear equations for the perturbations. We use a local approximation to investigate the one-dimensional radial propagation of Alfvén and high frequency electromagnetic waves. We derive the dispersion relation for these waves and solve it for the wave number k numerically.     

The Analytical Solution of Charged Black Hole and Calculation of Quantities (<Emphasis Type="Italic">T</Emphasis>,<Emphasis Type="Italic">s</Emphasis>,<Emphasis Type="Italic">f</Emphasis>)

One of the most remarkable features of black hole is the connection between properties of the classical solutions and thermodynamics. We include the electric and magnetic charges and this lead us to resolve Einstein equations. We obtain thermodynamic properties, such as temperature, entropy density and speed of sound with analytical solution. In that case we characterize equation of state in to V(φ) language.