生物分子拉曼散射测定过程中荧光背景的抑制

拉曼共焦技术、表面拉曼增强技术以及降低入射激光强度的方法，被运用于SOD、DNA等生物分子拉曼散身实验中，以抑制其荧光背景，改善光谱质量。     

火焰原子吸收法测定霞石中的氧化钾和氧化钠

本方法用于测定霞石中的钾、钠，主要通过在溶样时加入氢氟酸除去二氧化硅，调整溶液中钾、钠的含量，消除互相之间的干扰，加入氯化铯消除电离因素的干扰，提高了分析结果的准确性和重现性，使测定结果更接近化学分析值。回收率为99．2％-101．6％。     

吡罗红为底物的辣根过氧化物酶催化荧光反应测定葡萄糖

吡罗红在辣根过氧化物酶催化下可被过氧化氢氧化而使其荧光猝灭。在pH 7.2中性介质中,稳态催化速率由酶和底物浓度决定,催化体系服从Michaelis-Menten方程,用Lineweaver-Burk作图法求得米氏常数、最大反应速度、催化常数分别为2.4×10~(-5)mol·L-1,2.5×10~(-6)mol·L-1s-1,75.8 s-1。在最佳反应条件下,荧光F0/F的猝灭程度与过氧化氢浓度在0-3.6×10~(-7)mol·L-1范围内成线性关系,检测限为6.3×10~(-9)mol·L-1;当与葡萄糖氧化酶联用时,可定量检测葡萄糖,线性关系为0-8.0×10~(-7)mol·L-1,检测限为3.4×10~(-8)mol·L-1。方法用于分析人血清中葡萄糖含量,分析结果与苯酚-4-氨基安替比林法基本一致。     

Weighted oscillator strengths and lifetimes for the S IX and S X spectra

The weighted oscillator strengths (gf) and the lifetimes presented in this work were carried out in a multi configuration Hartree-Fock relativistic (HFR) approach. In this calculation, the electrostatic parameters were optimized by a least-squares procedure, in order to improve the adjustment to experimental energy levels. This method produces gf-values that are in better agreement with intensity observations and lifetime values that are closer to the experimental ones. In this work, we presented all the experimentally known electric dipole S IX and S X spectral lines.     

Differentiation between tracks and damages in SSNTD under the atomic force microscope

We have observed three-dimensional sponge-like structures as well as strips of connecting pits on the surface of the LR 115 detector after etching, which can be confused with the small tracks formed after short etching time. We have employed an atomic force microscope (AFM) to study these “damages” as well as genuine alpha tracks for short etching time. It was found that while the track and damage openings could be similar in size and shape, the depths for the damages were consistently smaller. Therefore, the depth of the pits will serve as a clear criterion to differentiate between tracks and other damages. The ability to discriminate between genuine tracks from other damages is most important for etching for short time intervals.     

Diffusion of 99Tc in granite under aerobic and anoxic conditions

Summary  Vezetéknév     

On an approach to the study of the Jaynes–Cummings sum in quantum optics

A new approach to the study of the Jaynes–Cummings sum, which determines the atomic inversion in quantum model of a single two-level atom interacting with a single mode of the quantized radiation field, based on the number theory theorems on approximation of trigonometric sums is presented.      

房水中烯醇化酶总活性的联酶荧光法测定

采用酶联反应荧光法测定样品中烯醇化酶的总活性。方法最低检出限为０２ＩＵ／Ｌ，在０２０～３５ＩＵ／Ｌ范围标准曲线呈线性，γ＞０９９７，回收率达９０％，相对标准偏差＜１０％。对临床确诊的视网膜母细胞瘤进行了初步探讨，检验结果的特异性较高。     

停流—荧光动力学分析法测定痕量钛的研究

利用自行组装的停流-荧光动力学分析仪,采用停流法汇合流体动力注入技术,研究了钛与3.5-二溴水杨基荧光酮的荧光增强动力学反应性质,讨论了流速、进样时间对方法灵敏度和精密度的影响,提出了一个快速、简便、精确测定钛的分析方法,并用于稀土样品及合成样品中痕量钛的测定.方法检测限为8×10~(-3)ppm,大多数元素的干扰均可用动力学方法消除.