纳米荧光小球标记在蛋白质微阵列检测中的应用研究

研究了不经分离、一次性制备氨基化联吡啶钌掺杂的双层二氧化硅纳米小球的方法。实验证明该纳米小球尺寸均匀,光稳定性、水溶性好,分散稳定。通过简单的偶联反应后,它能有效的和蛋白质结合,结合后的蛋白能保持其生物活性。以此纳米荧光小球为标记物,应用于蛋白质微阵列的定量检测,结果发现其效果明显优于相同条件下以异硫氰酸荧光素(FITC)为标记物的定量结果,检出限可以达到3.5 mg/L。     

ADSORPTION OF 2,4-D ON MODIFIED HYPERCROSSLINKED POLYSTYRENE （NDA-99） AND XAD-4 RESIN

The adsorption behavior of pesticide 2,4-dichlorophenoxyacetic acid (2,4-D) in aqueous solution has been investigated using a hypercrosslinked polystyrene adsorbent (NDA-99) modified by dimethylamine group as well as a nonionic macroporous adsorbent (XAD-4). The Langmuir and Freundlich isotherm models were employed to fit the experimental data to describe adsorption mechanism. It shows that NDA-99 resin exhibits an adsorption affinity for 2,4-D higher than XAD-4 resin owing to its exceptional micropore structure and the amine group of the hypercrosslinked matrix.Further studies indicate that the hydrogen bonding interaction and the stronger π-π conjugation play a significant role in the course of the adsorption of 2,4-D on NDA-99 resin, which is in agreement with the IR spectroscopic results and the △E values of HOMO (the highest occupied molecular orbit) of adsorbent and LUMO (the lowest unoccupied molecular orbit) of adsorbate calculated from the MINDO/3 model.     

Synthesis and Characterization of p—[Perfluoro—1—（2—fluorosulfonylethoxy）]ethylated Polystyrene

A new fluorinated polystyrene bearing a p-sulbstiuted perfluoro[1-(2-fluorosulfonylethoxy)]ethyl group was synthesized via one-electron oxidation of polystyrene by perfluoro[2-(2-fluorosulfonylethoxy)]propionyl peroxide at different peroxide to polystyrene molar ratios.The yield of perfluoroalkylation decreases with the increase of the reactant molar ratio.The modified polymer has been characterized by various techniques:the ring pefluoro[1-(2-fluorosulfonylethoxy)]ethylation has been proved by FT-IR and ^19FNMR;the X-ray photoelectron spectra(XPS) show the maximum binding energy of F18,O18,C18(two kinds of carbon atoms,namely C-H and C-F)and S2p,respectively; desulfonylation of the fluorinated polystyrene appearing at 217℃ has been found by its thermogravimetric analysis (TGA).The determinations of contact angle,refractive index and glass transition temperature of the modified polymer have disclosed that when the contact angle increases with the increase of the molar ratio,the refractive index and glass transition temperature decrease.The polydispersity values indicate that the degradation of the polymer chains did not occur during the reaction.     

DIFFUSION OF LONG CHAINS THROUGH A POROUS THIN GEL NETWORK

Using a core(142 nm)made of linear polystyrene(PS)chains as a seed,we further polymerized a thin layer of cross-linked PS shell(7 nm)on it in water to form a core-shell particle.Such a particle swells in toluene,which enables linear PS chains inside the core to gradually diffuse out through the porous shell.Using a combination of static and dynamic laser light scattering,we examined the chain diffusion process by following the change of the scattering intensity(i.e.the average molar mass of the particles).For the first time,we have revealed that the diffusion exhibits three stages.In the first stage,the chain diffusion through the shell is even faster than their translational diffusion in a dilute solution.The three stages respectively correspond to the change of the solution in the core from concentrated to semidilute and then from semidilute to dilute.     

聚苯乙烯/蒙脱土熔融插层复合的研究

用熔融法制备了聚苯乙烯／蒙脱土插层复合材料，用Ｘ 射线衍射、ＤＳＣ等手段研究了复合材料的结构与性能．聚苯乙烯熔体不能插层于钠基蒙脱土中，但能插层于经有机化合物处理过的蒙脱土中．     

PHOTOPHYSICAL STUDY OF SULFONATED POLYSTYRENE

The photophysical properties of a series of sulfonated micromolecule(paratoluenesulfonic acid,HPTS) and macromolecules (linear and crosslinked poiys.tvrene) have been studied by steady-state fluorescence spectra. The results indicate that the ground sulfonated ring associations can form in both the micromolecules and the macromolecules. The fluorescence spectra of the sulfonated crosslinked copolymers appear a red-shift when the copolymers change from hydrogen-type to sodium-t~pe, and some new emission bands appear in the long-wavelength region.These results are explained in terms of synergetic effect of hydrogen bond, π-π interaction and crosslinking effect.     

超临界苯类溶剂对聚苯乙烯降解的影响

在高压间歇反应器中,温度340~370℃,以苯、甲苯、乙苯和对二甲苯为超临界溶剂研究了聚苯乙烯(PS)的降解特性.苯类物质是聚苯乙烯的优良溶剂,在超临界条件下其优异的传质、传热性能使聚苯乙烯快速降解.聚苯乙烯在不同超临界溶剂中降解转化率相近,而降解产物组成差别很大,分析了不同超临界溶剂对聚苯乙烯降解过程的影响.结果表明超临界甲苯对降解过程影响最小,苯乙烯收率最高.聚苯乙烯降解过程中,高分子链断裂和解聚同时进行,结合连续分布理论建立了聚苯乙烯降解的动力学模型,得到在超临界甲苯中聚苯乙烯链端解聚活化能为138.4 kJ.mol-1.     

环形聚苯乙烯的稀溶液性质——Ⅱ. 第二维利系数

第二维利系数(A_2)是表征高分子链段间和高分子溶剂分子间相互作用的参数。对于环形高分子的第二维利系数,最早Casassa曾做过计算。利用“双接触”模型,Casassa计算了环形高分子的第二维利系数(A_(2r))为A_(2r)=(N_αβN~2/2M~2)[1-C_z O(z~2)]式中M为聚合物的分子量,N_α为Avogadro常数,N为高分子链段数,β为表征链段对排斥体积的二元集团积分,Z为排斥体积参数。式中系数C对环形或线形分子数值不同。     

聚苯乙烯型离子交换树脂的研究进展

介绍了聚苯乙烯型离子交换树脂的合成方法;综述了近年来在氯甲基化反应、Mannich反应以及磺化反应上的新进展、新理论：从结构上对聚苯乙烯型离子交换树脂的强度和热稳定性进行了分析。聚苯乙烯型离子交换树脂具有稳定的物理化学性质、吸附选择独特、再生容易、操作简便、使用周期长等优良性能,大大促进了化工企业、制药工业、环保、医疗、分析等行业的发展,具有广阔的发展前景。     

亚微米聚苯乙烯微粒粒度标准物质研制

介绍亚微米聚苯乙烯微粒粒度标准物质的研制过程.以苯乙烯为原料,采用无皂乳液聚合法和分散聚合一步合成法制备粒径分别为0.3、0.4、0.5、0.6、0.7、0.8、0.9、1μm的8种规格聚苯乙烯微粒粒度标准物质,用扫描电子显微镜测定颗粒的粒径,对标准物质的均匀性和稳定性进行检验,并对定值结果的不确定度进行评定.研制的聚...