Expression and Characterization of Catalytic Domain of T Cell Protein Tyrosine Phosphatase（△TC-PTP） —— Immunohistochemical Study of △TC-PTP Expression in Non-small Cell Lung Carcinomas

This study objective was to express and characterize the catalytic domain of the human T cell protein tyrosine phosphatase（△TC-PTP） and to study immunohistochemically the expression of △TC-PTP in human non-small cell lung cancers. △TC-PTP gene was PCR amplified with the cDNA of human TC-PTP as template, and cloned into the pT7 expression vector. The recombinant pT7-△TC-PTP was expressed in E. coli Rosetta （ DE3 ） host cells and puri- fied. The enzymatic characteristics of △TC-PTP including enzyme activity and kinetics assay were measured. The antiserum was prepared by immunizing rabbit with the purified recombinant △TC-PTP. Rabbit polyclonal antibody against △TC-PTP was purified by PVDF immobilized antigen affinity chromatography. Immunohistochemical staining of lung cancer tissues was performed with antibody against △TC-PTP protein. △TC-PTP gene was correctly cloned, expressed, and purified. The recombinant △TC-PTP had a highly catalytic activity of PTPase. Squamous cell lung carcinoma showed a significantly higher expression rate of △TC-PTP （76. 92%, 10/13 ） than adenocarcinoma （57.14%, 4/7） and normal lung tissue（20%, 1/5 ）. This study represents the first demonstration that △TC-PTP is highly expressed in human squamous cell lung carcinomas. In addition, this study provides an important basis for further studying the biological function of TC-PTP and its relationship with lung carcinomas and other diseases.     

Synthesis and Crystal Structure of 3-（Trichloroacetyl） Indole

1 INTRODUCTION Indole and its derivatives have attracted much at-tention due to their chemical properties as well asbiological activities[1, . They have been widely used 2]as the materials for producing pigment, perfume,plant growth regulators, etc. Recently, it has alsobeen found that some indole derivatives present anti-tumor and antiviral activities[3～5]. During our sear-ches for bioactive compounds, a series of indole deri-vatives were synthesized, among …     

Influences of Triton X-100 on Hemoglobin Behaviors in Hemoglobin/Acyclovir/Triton X-100/H2O System

The influences of Triton X-100 on hemoglobin （Hb） behaviors were studied by the methods of UV-Vis spectrum, fluorescence spectrum, HPLC, conductivity, zeta potential and negative-staining transmission electron microscope in Hb/acyclovir/Triton X-100/H2O system. With the increase of Triton X-100 concentration in the system, the percentage of the free acyclovir increased from 58%--63% to 90%--94%. The static quenching constant and the association number of acyclovir to Hb decreased. The fluorescence spectrum, conductivity, zeta potential, fluorescence polarization and negative-staining morphology of Hb tended to recover to those of the original state of Hb in the same concentration of Hb. The interaction between Triton X-100 and Hb is stronger than that between acyclovir and Hb. Most Triton-X-100 was associated with Hb at low Triton X-100 concentration. But the interaction of Triton X-100 with Hb was apparently dominant in high Triton X-100 concentration. The Hb structure was unfolded and finally denatured.     

Mg-Fe-HTIc／高岭土悬浮体流变性研究

研究了Mg—Fe类水滑石(HTIc)／高岭土(kaolinite)悬浮体的质量比（R)对其流变性和触变性的影响，发现控制剪切速率(D)得到的屈服应力(τy)和控制剪切应力(τ)得到的临界剪切应力(τc)皆随R拘增大先降低后升高．小幅振荡剪切实验发现随R的增大，体系由正触变性转化为复合触变性，其中用小幅振荡剪切法在线性粘弹区发现复合触变性体系还是首次．恒剪切速率(DL)实验发现了震荡现象，即粘度随时间发生周期性升高和降低的变化．震荡现象与DL和R有关，较高的DL和尺可使震荡现象消失．测定粘度随时间变化时采用的低剪切速率(DL)影响体系的触变性类型．根据HTIc和kaolinite粒子间的相互作用对实验结果的机理进行了探讨．     

高阻隔材料——偏氯乙烯悬浮共聚树脂

本文介绍偏氯乙烯悬浮共聚树脂的优异阻隔性能、合成配方与工艺。     

The Surface Imprinted Polystyrene Beads Prepared via Emulsion Templates

In this paper, the surface imprinted cross-linked polystyrene beads were prepared via suspension polymerization with styrene (St), divinylbezene (DVB), polyvinyl alcohol (PVA1788),the mixture of Span 85 and xylene or the mixture of Span 85 and paraffin as monomer,cross-linking agent, dispersion stabilizer and templates, respectively. The results indicate that there are dense cavities on the surface of beads, and the diameter and density of cavity are related with the composition and amount of emulsion template. The forming mechanism of cavity from thermodynamics and dynamics was proposed.     

CaBPO5：RE（RE=Eu，Tb）的水热合成及其发光特性

利用水热法合成了CaBPO5：RE(RE=Eu，Tb)荧光体并测试了其结构和光谱，讨论了其发光性质，并与高温固相法合成的产物作了对比．结果表明，由于电子转移。Eu^3 ,Tb^3 和Eu^2 共存于同一体系中，而且Eu^2 的发射位置从402nm移至428nm,在双掺杂体系中引入Ce^3 ．Eu^3 ，Tb^3 和Eu^2 的发光强度均有所增强,这可能是Ce^3 与Eu^3 之间的电子转移及各种稀土离子之间能量传递相互竞争的结果。     

BaZrxTi1-xO3·yLa2O3固溶体的合成、结构与性能

在100℃以下用软化学合成方法制备了一系列BaZrxTi1-xO3·yLa2O3O(0≤x≤0.3,0≤y≤0.04)固溶体纳米粉末.XRD物相分析及d-间隔-组成图证明,产品为完全互溶取代固溶体.从TEM谱图观察,粒子为均匀球形,平均粒径60nm.通过制陶实验对该系列固溶体的介电特性进行了测试,结果表明,采用化学掺杂方法在BaTiO3中掺入适量锆和镧后,样品的室温介电常数可提高20倍.     

The Unusual Spectrum of the Mixture of Tin-octabutoxy Naphthalocyanine and SnCl2 in CH2Cl2

The Q-band position of tin-centered 5, 9, 14, 18, 23, 27, 32, 36-octabutoxy 2, 3-phthalocyanine(SnNc(OBu)8) exhibits dramatic red-shift as mixed with SnCl2 in CH2Cl2.