叶酸受体介导的肿瘤细胞靶向分子显像剂的研究进展

叶酸受体在肿瘤细胞中都有过度表达,通过其介导细胞内化能够将叶酸摄取入细胞浆内,因此利用叶酸受体与叶酸及其类似物偶联的分子显像剂特异性结合,可以达到肿瘤组织靶向分布和显像目的,在肿瘤诊断和治疗方面很有前景。本文根据近年来的文献资料,对叶酸受体介导的靶向分子显像剂的研究进展作了概述。     

应用镧探针对不同心功能心脏病人活检心肌通透性观察

在心力衰竭发生和演变过程中,心肌细胞膜的损伤程度起重要的作用,以往的研究证实镧 离子可作为心肌通透性的示踪元素,本文应用镧探针对四种不同心功能心脏病人及健康人活检心肌的通透性进行对比性研究,发现镧颗粒以不同程度渗透进入心肌细胞膜,肌原纤维及接的肌膜的线粒体,它们的通透程度与心功能状态一致,提示这些改变对了解心力的机制十分重要。     

Ce3+对大白鼠和豚鼠心肌的影响

研究了Ｃｅ＾３＋对离体豚鼠心房收缩,离体豚鼠乳头肌及在体大白鼠心肌动作电位的影响。浓度为０．０５ｍｍｏｌ．Ｌ＾－１的Ｃｅ＾３＋溶液可抑制豚鼠右心房的自律性和左心房的收缩。０．１和０．２ｍｍｏｌ．Ｌ＾－１Ｃｅ＾３＋溶液对豚鼠乳头肌动作电位时程影响不显著。     

99mTcN-MIBI在体外肿瘤细胞内的摄取及其与99mTc-MIBI的比较研究

研究了结肠癌细胞CL-187、肺腺癌细胞L-973以及乳腺癌细胞MCF-7分别对^99mTcN-MIBI和^99mTc-MIBI配合物的摄取情况.结果表明,^99mTcN-MIBI在3种肿瘤细胞内培养20～40min时的摄取达到极大值,并且摄取量明显高于^99mTc-MIBI,特别是在结肠癌细胞CL-187中.^99mTcN-MIBI在乳腺癌细胞MCF-7内的摄取量最高,培养40min时的单位细胞摄取百分率约为30%/105个细胞;在结肠癌细胞CL-187内的摄取量次之;在肺腺癌细胞L-973内的摄取量最低.细胞摄取条件实验结果表明:细胞浓度和培养温度的变化均会对细胞摄取产生影响.     

肌钙蛋白Ⅰ提取纯化新方法的研究

兔骨骼肌匀浆液经离心后,依次经DEAE－SephadexA25,SephadexG75及ButylSepharoseF.F柱层析,得到了兔骨骼肌肌钙蛋白C(sTnC)纯品.将其与Sepharose4B凝胶偶联,制成sTnC亲和层析凝胶.人心肌或骨骼肌经匀浆、离心后上sTnC亲和层析柱,一步分离可获得人心肌肌钙蛋白Ⅰ(cTnI)或人骨骼肌肌钙蛋白Ⅰ(sTnI)纯品.HPLC分析出现单一峰.SDS－PAGE分析得到一条电泳带,其分子量分别为25000及21000.用20gsTnC亲和凝胶处理40g人心肌时,相当于每100g心肌可得到82.6mgcTnI.     

反相高效液相色谱法测定核苷酸代谢产物

A method for the determination of high energy nucleotide′s metabolites of rabbit′s heart tissue by RP-HPLC with UltrasphereTM-C18 column at 254nm was established.The CVs were 1.4～8.8%。 The lowest de-tection limits were 6.25ng.This method is sensitive,reliable and suitable for the determination of nucleotide and it′s metabolites。     

红外激光心肌打孔的理论模型和实验验证

根据激光与心肌组织的作用规律和热力学理论建立了红外激光心肌打孔的理论模型,推导出红外激光心肌打孔的深度和打孔时间之间的定量关系.并用100 W连续输出的CO2激光在猪的心脏上进行了激光心肌打孔实验,实验结果和理论推导结果在一定范围内能够较好地吻合.     

4-取代环芬尼类化合物的合成及标记

以Cyclofenil为原料, 合成得到一系列氟乙氧基衍生物. 通过受体结合实验, 筛选得到一个对雌激素受体(ER)具有高特异性和高相对亲和力(RBA)的化合物Penta-Cyclofenil-FEt. 通过实验的优化设计, 合成得到相应的氟标记前体, 产物通过IR, UV, ¹H NMR, ¹⁹F NMR或ESI-HRMS进行了表征. 在冷试条件下, 得到了冷氟取代参比化合物. 在热试验条件下, 得到F-18标记产物. 此类非甾体雌激素受体分子探针的合成, 为PET (positron emission tomography)显像雌激素受体β从而评估乳腺癌的恶化程度奠定了基础.     

A generalized method of converting CT image to PET linear attenuation coefficient distribution in PET/CT imaging

The accuracy of attenuation correction in positron emission tomography scanners depends mainly on deriving the reliable 511-keV linear attenuation coefficient distribution in the scanned objects. In the PET/CT system, the linear attenu- ation distribution is usually obtained from the intensities of the CT image. However, the intensities of the CT image relate to the attenuation of photons in an energy range of 40 keV-140 keV. Before implementing PET attenuation correction, the intensities of CT images must be transformed into the PET 511-keV linear attenuation coefficients. However, the CT scan parameters can affect the effective energy of CT X-ray photons and thus affect the intensities of the CT image. Therefore, for PET/CT attenuation correction, it is crucial to determine the conversion curve with a given set of CT scan parameters and convert the CT image into a PET linear attenuation coefficient distribution. A generalized method is proposed for con- verting a CT image into a PET linear attenuation coefficient distribution. Instead of some parameter-dependent phantom calibration experiments, the conversion curve is calculated directly by employing the consistency conditions to yield the most consistent attenuation map with the measured PET data. The method is evaluated with phantom experiments and small animal experiments. In phantom studies, the estimated conversion curve fits the true attenuation coefficients accurately, and accurate PET attenuation maps are obtained by the estimated conversion curves and provide nearly the same correction results as the true attenuation map. In small animal studies, a more complicated attenuation distribution of the mouse is obtained successfully to remove the attenuation artifact and improve the PET image contrast efficiently.