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Changes in binding affinity to catalytic antibody 6D9 of chloramphenicol phosphonate derivatives (CPDs) containing H or F were investigated by performing free energy calculations based on molecular dynamics simulations. We calculated the binding free energy, enthalpy, and entropy changes (DeltaDeltaG, DeltaDeltaH, and -TDeltaDeltaS) attributable to H-->F substitution by comparing results for CPDs containing a trifluoroacetylamino group (CPD-F) or an acetylamino group (CPD-H). The calculated DeltaDeltaG, DeltaDeltaH, and -TDeltaDeltaS values were -2.9, -6.3, and 3.5 kcal mol(-1) and close to experimental values observed for a series of similar ligands, chloramphenicol phosphonates with F and H (-1.4, -3.5, and 2.1 kcal mol(-1)). Therefore, CPD-F binds more strongly to 6D9 than does CPD-H. To clarify the origin of the large difference in DeltaDeltaG, we apportioned the calculated values of DeltaDeltaG and DeltaG for the associated and dissociated states into contributions from various atomic interactions. We found that the H-->F substitution increased the binding affinity mainly by decreasing the hydration free energy and not by increasing favorable interactions with the antibody. The decreased hydration free energy of the ligand was mainly due to unfavorable coulombic interactions between the trifluoroacetylamino group and solvent waters, which increased the free energy of the dissociated state (by about 3.7 kcal mol(-1)). Also, the trifluoroacetylamino group slightly increased the free energy level of the associated state (about 0.8 kcal mol(-1)) because favorable van der Waals interactions compensated for unfavorable coulombic interactions with antibody atoms. In addition, the enthalpy and entropy changes, DeltaDeltaH and -TDeltaDeltaS (computationally -6.3 and 3.5 kcal mol(-1)), originated mainly from a decrease in hydration free energy in the dissociated state. The CPD-F and CPD-H ligands had substantially different structures in the dissociated and complexed states. 相似文献
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Advanced Sensing of Antibiotics with Magnetic Gold Nanocomposite: Electrochemical Detection of Chloramphenicol
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Dr. Petr Jakubec Dr. Veronika Urbanová Dr. Zdenka Medříková Prof. Dr. Radek Zbořil 《Chemistry (Weinheim an der Bergstrasse, Germany)》2016,22(40):14279-14284
The sensing and accurate determination of antibiotics in various environments represents a big challenge, mainly owing to their widespread use in medicine, veterinary practice, and other fields. Therefore, a new, simple electrochemical sensor for the detection of antibiotic chloramphenicol (CAP) has been developed in this work. The amplification strategy of the sensor is based on the application of magnetite nanostructures stabilized with carboxymethyl cellulose (Fe3O4‐CMC) and decorated with nanometer‐sized Au nanoparticles (NPs) (Fe3O4‐CMC@Au). In this case, CMC serves as a stabilizing agent, preventing the aggregation of Fe3O4 NPs, and hence, enabling the kinetic barrier for electron transport to be overcome, and the Au NPs serve as an electron‐conducting tunnel for better electron transport. As a proof of concept, the developed nanosensor is used for the detection of CAP in human urine samples, giving a recovery value of around 97 %, which indicates the high accuracy of the as‐prepared nanosensor. 相似文献
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Hweiyan Tsai Hsiao-Ching Hu Chih-Chung Hsieh Yi-Hsuan Lu Chien-Hong Chen Chwan-Bor Fuh 《中国化学会会志》2020,67(1):152-159
Chloramphenicol (CAP) is a veterinary antibiotic that has been banned due to its severe side effects in humans. Through the application of manure, veterinary antibiotics can enter the soil, where they can be taken up by crops and vegetables and pose a potential health hazard to humans. Thus, it is highly desirable to develop a rapid and sensitive tool for on-site detection of CAP to ensure food safety and to control the abuse of antibiotics. To this end, nitrogen-doped graphene quantum dots (N-GQDs) were successfully prepared via microwave-assisted synthesis using citric acid and urea as carbon and nitrogen sources, respectively. Analytical results suggested that the interaction between N-GQDs and CAP could occurs via π-π stacking, which quenched N-GQD fluorescence. CAP spiked into chicken feed could be rapidly extracted with ethanol and quantified based on N-GQD fluorescence quenching without further separation. This method showed good recovery (97–102.6%), a low detection limit (1.8 ppm), and was not affected by interference from florfenicol, and thiamphenicol, legal substitute antibiotics. This method has excellent potential for determination of CAP in livestock feed and soil. 相似文献
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高效液相色谱/串联质谱法同时测定虾中氯霉素、甲砜霉素和氟甲砜霉素残留量 总被引:28,自引:0,他引:28
用高效液相色谱/串联质谱(LC/MS,/MS)同时测定虾中的氯霉素(CAP)、甲砜霉素(TAP)和氟甲砜霉素(FF)。均质后的虾样品,采用碱化乙酸乙酯提取。浓缩提取物经液.液分配(LLP)去除脂肪,C18固相萃取(SPE)柱净化后,采用LC/MS/MS电喷雾电离(ESI),负离子,多反应监测(MRM)模式检测,外标法定量。检出限为:氯霉素和氟甲砜霉素0.01ng/g;甲砜霉素为0.05ng/g。在添加浓度0.1~2.0ng/g范围内,氯霉素回收率为73.9%~96.0%;甲砜霉素回收率为78.6%~99.5%;氟甲砜霉素回收率为74.9%~103.7%;相对标准偏差(RSD)均小于6.4%。 相似文献
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《Magnetic resonance in chemistry : MRC》2003,41(7):489-502
Biological reactions are mostly concerned with selective interactions between small ligands and macromolecular receptors. The same ligands may activate responses of different intensities and/or effects in the presence of different receptors. Many approaches based on spectroscopic and non‐spectroscopic methods have been used to study interactions between small ligands and macromolecular receptors, including methods based on NMR and IR spectroscopic analysis of the solution behaviour of the ligand in the presence of receptors. In this work, we investigated the interaction between ovine serum albumin with two amphenicolic antibiotics [chloramphenicol (CAP) and thiamphenicol (TAP)], using a combined approach based on NMR and IR methodologies, furnishing complementary information about the recognition process occurring within the two systems. The two ligands, despite their similar structures, showed different affinities towards albumin. NMR methodology is based on the comparison of selective ( ) and non‐selective ( ) spin–lattice relaxation rates of the ligands in the presence and absence of macromolecular receptors and and temperature dependence analysis. From these studies, the ligand–receptor binding strength was evaluated on the basis of the ‘affinity index.’ The derivation of the affinity index from chemical equilibrium kinetics for both the CAP–albumin and TAP–albumin systems allowed a comparison of the abilities of the two amphenicolic antibiotics to interact with the protein. IR methodology is based on the comparison of the ligand–protein ‘complex’ spectra with those of the non‐interacting systems. On the basis of the differences revealed, a more thorough IR analysis was performed in order to understand the structural changes which occurred on both ligand and protein molecules within the interacting system. Copyright © 2003 John Wiley & Sons, Ltd. 相似文献
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Pei L Bao Y Wang H Yang F Xu B Wang S Yang X Du G 《Biomedical chromatography : BMC》2008,22(7):786-794
Salvianolic acid A (SAA), a major effective constituent of Salvia miltiorrhizas, is widely used in traditional Chinese medicine. A sensitive rapid analytical method was established and validated for SAA in rat plasma, which was further applied to assess the pharmacokinetics of SAA in rats receiving a single oral dose of SAA. The method used liquid chromatography tandem mass spectrometry in multiple reaction monitoring mode with chloramphenicol as the internal standard. A simple liquid-liquid extraction based on ethyl acetate was employed. The combination of a simple sample cleanup and short chromatographic run time (3 min) increased the throughput of the method substantially. The method was validated over the range 1.4-1000 ng/mL with a correlation coefficient >0.99. The lower limit of quantification was 1.4 ng/mL for SAA in plasma. Intra- and inter-day accuracies for SAA were 95-113 and 98-107%, and the inter-day precision was less than 12%. This method is more sensitive and faster than previous methods. After a single oral dose of 100 mg/kg of SAA, the mean peak plasma concentration (Cmax) of SAA was 318 ng/mL at 0.5 h, the area under the plasma concentration-time curve (AUC0-12 h) was 698 +/- 129 ng.h/mL, and the elimination half-life (T1/2) was 3.29 h. 相似文献
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采用静电纺丝的方法制备了不同质量分数的CUR-CAP/PLGA复合静电纺丝纤维膜,并通过扫描电子显微镜、红外光谱、单晶X射线衍射进行表征,并用四唑盐MTT比色法测定其对成纤维细胞增殖的影响.表征结果表明:所合成的复合静电纺丝纤维膜的直径具有纳米结构,复合薄膜中,3种物质很好的混合在一起,没有发生化学键的结合.细胞实验检测结果显示,CUR-CAP/PLGA复合膜有助于细胞在其表面的生长及增殖,当姜黄素的质量分数为3%、氯霉素的质量分数为1%时效果最佳. 相似文献
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高效液相色谱串联质谱测定蜂蜜、蜂王浆中氯霉素残留 总被引:7,自引:0,他引:7
前处理方法包括添加同位素内标氯霉素-d5和采用10%偏磷酸沉淀蜂王浆产品中的蛋白质,上清液经乙酸乙酯提取,自制硅胶柱和Oasis小柱净化。净化后的提取溶液用高效液相色谱-电喷雾电离质谱检测,多反应监测3对离子(321.0/256.9、321.0/194.0、321.0/175.8)。该方法对不同基质样品的加标回收率为91%-107%;相对标准偏差小于10%;蜂蜜和蜂王浆的方法检出限分别为0.1μg/kg和0.2μg/kg。 相似文献
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The coupling of chloramphenicol on Biozan R (Hercules) by activation with dicyclohexylcarbodiimide was studied. The esterification is influenced by the chloramphenicol/dicyclohexylcarbodiimide ratio, concentration of the reactants and reaction time.The microbial tests performed with products of different chloramphenicol contents attest their antimicrobial activity and their possible use, especially in ophtalmology. 相似文献