三维荧光二阶校正法用于复杂基体样中川芎嗪和替米沙坦的快速定量分析

采用三维荧光光谱法,结合化学计量学中基于交替归一加权残差(ANWE)算法的二阶校正方法,实现了人血浆样品中川芎嗪和替米沙坦的同时快速定量分析.当组分数N取4时,ANWE算法解析获得的川芎嗪和替米沙坦的平均回收率分别为99.3士3.5%和96.8±2.0%.本文同时给出了此方法的检测限(LOD)与定量下限(LOQ),获得...     

基于嵌入式PC/104的狭缝摄影控制系统

介绍狭缝摄影和以PC／104作平台的狭缝摄影控制系统的工作原理，给出了控制系统硬件组成及软件设计方法。     

A meshless method using the radial basis functions for numerical solution of the regularized long wave equation

This article discusses on the solution of the regularized long wave (RLW) equation, which is introduced to describe the development of the undular bore, has been used for modeling in many branches of science and engineering. A numerical method is presented to solve the RLW equation. The main idea behind this numerical simulation is to use the collocation and approximating the solution by radial basis functions (RBFs). To avoid solving the nonlinear system, a predictor‐corrector scheme is proposed. Several test problems are given to validate the new technique. The numerical simulation, includes the propagation of a solitary wave, interaction of two positive solitary waves, interaction of a positive and a negative solitary wave, the evaluation of Maxwellian pulse into stable solitary waves and the development of an undular bore. The three invariants of the motion are calculated to determine the conservation properties of the algorithm. The results of numerical experiments are compared with analytical solution and with those of other recently published methods to confirm the accuracy and efficiency of the presented scheme.© 2009 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq 2010     

Microstructure,Interfacial Interactions,and Rheological Properties of PC/AES/Montmorillonite Composites

Polycarbonate (PC) and acrylonitrile–EPDM (ethylene/propylene/diene elastomer)–styrene ter‐polymer (AES) blends and PC/AES/organically modified montmorillonite (OMMT) composites were prepared at 20%, 40%, 50% by weight of AES and 3% by weight of OMMT. The microstructure, interfacial interactions, and rheological properties of the PC/AES blends and PC/AES/OMMT composites were studied systematically. X‐ray diffractometer (XRD) results reveal that the AES is easier to intercalate into OMMT than PC, and the content of AES has a little effect on the interlayer distance of OMMT. Wetting coefficient calculation indicates that OMMT distributes primarily at the interface of the polymer blend. Field emission scanning electron microscope (FE‐SEM) observation indicates that the phase morphology of PC/AES blends and PC/AES/OMMT composites is not influenced by the OMMT. However, linear rheological properties suggest that the addition of OMMT has a great effect on the linear rheological property.     

Comparison between blue lasers and light‐emitting diodes for future solid‐state lighting

Solid‐state lighting (SSL) is now the most efficient source of high color quality white light ever created. Nevertheless, the blue InGaN light‐emitting diodes (LEDs) that are the light engine of SSL still have significant performance limitations. Foremost among these is the decrease in efficiency at high input current densities widely known as “efficiency droop.” Efficiency droop limits input power densities, contrary to the desire to produce more photons per unit LED chip area and to make SSL more affordable. Pending a solution to efficiency droop, an alternative device could be a blue laser diode (LD). LDs, operated in stimulated emission, can have high efficiencies at much higher input power densities than LEDs can. In this article, LEDs and LDs for future SSL are explored by comparing: their current state‐of‐the‐art input‐power‐density‐dependent power‐conversion efficiencies; potential improvements both in their peak power‐conversion efficiencies and in the input power densities at which those efficiencies peak; and their economics for practical SSL.     

一种低成本线阵CCD驱动与数据采集处理系统的研究

探索了一种在低成本下实现线阵CCD输出信号快速采集以及与PC通信的方法。讨论了线阵CCD系统的整体结构及工作流程。采用VHDL语言编写了驱动程序,实现了CCD的正确工作。利用DSP实现了对下位CCD的控制,在控制程序中利用"乒乓原理"实现了线阵CCD的数据采集,并同时给PC快速上传数据。基于Visual C++6.0平台编写了PC机控制软件,实现了对硬件电路的控制和对CCD输出信号的分析处理。     

Tricin isolated from Allium atroviolaceum potentiated the effect of docetaxel on PC3 cell proliferation: role of miR-21

For more effectiveness and overcoming the drug resistance Chemotherapy agents, the combination treatment is raised. Flavonoids with different anti-cancer effects are an appropriate choice as lead compounds. Over expressed MiR-21 in prostate cancer is associated with metastasis and drug resistance to chemotherapy with Docetaxel. In this study, the anticancer effect of 4′, 5, 7-Trihydroxy-3′, 5′-dimethoxyflavone (Tricin) was investigated with Docetaxel on PC3 cell line. Tricin was initially isolated from the Allium atroviolaceum by column chromatography and recrystallization method. The chemical structure of isolate was elucidated by spectroscopic techniques. IC₅₀ of Tricin and Docetaxel were assessed 117.5 ± 4.4 μM and 0.1 ± 0.02 nM by MTT assay, respectively. Analysis of results indicates the synergistic effect of combination therapy on decreased proliferation. MiR-21 in treated cells with Tricin significantly decreased compared to control cells. So, Tricin can be effective in the reduction of metastasis and drug resistance of Docetaxel.     

Synergistic therapeutic effects of Duzhong Jiangya Tablets and amlodipine besylate combination in spontaneously hypertensive rats using 1H-NMR- and MS-based metabolomics

Duzhong Jiangya Tablet (DJT) composed of Eucommia ulmoides Oliv. and several other traditional Chinese medicines is a Chinese herbal compound, which is clinically used to treat hypertension. The aim of this study was to evaluate the antihypertensive effect of DJT and amlodipine besylate (AB) on the synergistic treatment of spontaneously hypertensive rats (SHRs), and to explore its antihypertensive mechanism. The synergistic therapeutic effect of DJT in combination with AB on SHR was studied using two metabolomics methods based on mass spectrum (MS) and nuclear magnetic resonance. Metabolomics analysis of plasma, urine, liver, and kidney and the combination of orthogonal partial least squares discriminant analysis was performed to expose potential biomarkers. Then, the overall metabolic characteristics and related abnormal metabolic pathways in hypertensive rats were constructed. Blood pressure measurements showed that DJT combined with AB has better effects in treating hypertension than it being alone. A total of 30 biomarkers were identified, indicating that hypertension disrupted the balance of multiple metabolic pathways in the body, and that combined administration restored metabolite levels better than their administration alone. The changes of biomarkers revealed the synergistic therapeutic mechanism of DJT combined with AB, which provided a reference for the combination of Chinese and Western medicines.     

Precise analysis of thyroxine enantiomers in pharmaceutical formulation by mobility difference based on cyclodextrin

Identification and determination of chiral pharmaceutical residues is still a challenging analytical puzzle. In this work, a simple, rapid, and effective method for chiral D/L-tetraiodothyronine (T4) separation and quantitative was developed based on host–guest recognition using ion mobility spectrometry-mass spectrometry (IMS-MS). The D/L-T4 enantiomers were mobility separated by their diastereomeric complexes through mixing with cyclodextrin (CD) and metal ions. D/L-T4 was first separated by complexing with host molecule (α-, β-, γ-CD), observing weak peak-to-peak resolution (R_p-p) by the formed binary complex [CD + D/L-T4-H]⁺, and the R_p-p decreased with the CD size increasing. However, the separation effect of D/L-T4 was much improved with the addition of divalent metal ions (G²⁺) by the formed ternary complex [CD + D/L-T4 + G]²⁺. In comparison, α-CD related complexes can possess the best separation effect for D/L-T4 in most cases. Considering the high selectivity, non-toxic, and chemically stable of β-CD, [β-CD + D/L-T4 + Ca]²⁺ was selected for D/L-T4 analysis (R_P-P = 0.764). Whereafter, chemical theoretical conformations for [β-CD + D/L-T4 + H]⁺ and [β-CD + D/L-T4 + Ca]²⁺ were optimized, discovering similar micro-interaction modes between [β-CD + D-T4 + H]⁺ and [β-CD + L-T4 + H]⁺; while with the addition of Ca²⁺, significantly different interaction modes were observed between [β-CD + D-T4 + Ca]²⁺ and [β-CD + L-T4 + Ca]²⁺. And theoretical collision cross section (CCS) trends for the complexes were consistent with that of the experimental results. Additionally, calibration curves were linear within 1.00 to 10⁴ ng mL^?1 with coefficient (R² > 0.99), gaining the limit of detection (LODs) calculation of 0.11 ng mL^?1, and the detection range between D-T4 and L-T4 of 45.6:1 to 1:59.8. Finally, the method was applied for D/L-T4 detection in Levothyroxine tablets, the detection content has good consistency on drug labeling. Because the proposed method exhibited good analytical performance in terms of speed, selectivity, sensitivity, and reproducibility of the measurements, that can be a promising strategy for effective D/L-T4 detection in pharmaceutical industries or other practical samples.