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81.
Fan Liu Yi Shi Yakun Fang Zhenshan Liu Yu Xin Zhenghua Gu Zitao Guo Liang Zhang 《Molecules (Basel, Switzerland)》2022,27(19)
As a biologically active peptide, L-carnosine has been widely used in the pharmaceutical, cosmetic and health care industries due to its various physiological properties. However, relatively little research is available regarding L-carnosine’s enzymatic synthesis function. In this study, a potential enzyme sequence with the function of carnosine synthesizing was screened out using the ancestral sequence reconstruction (ASR) technique. Identified with L-carnosine synthesis activity, this enzyme was further confirmed using autoproteolytic phenomenon via Western blot and N-terminal sequencing. After purification, the enzymatic properties of LUCA–DmpA were characterized. The melting temperature (Tm) and denaturation enthalpy (ΔH) of LUCA–DmpA were 60.27 ± 1.24 °C and 1306.00 ± 26.73 kJ·mol−1, respectively. Circular dichroism (CD) spectroscopy results showed that this ancestral enzyme was composed of α-helix (35.23 ± 0.06%), β-sheet (11.06 ± 0.06%), β-turn (23.67 ± 0.06%) and random coil (32.03 ± 0.06%). The enzyme was characterized with the optimal temperature and pH of 45 °C and 9.0, respectively. Notably, LUCA–DmpA was also characterized with remarkable pH tolerance based on the observation of more than 85% remaining enzymatic activity after incubation at different pH buffers (pH = 6–11) for 12 h. Additionally, rather than being improved or inhibited by metal ions, its enzymatic activity was found to be promoted by introducing organic solvent with a larger log P value. Based on these homology modeling results, the screened LUCA–DmpA is suggested to have further optimization potential, and thereafter to be offered as a promising candidate for real industrial applications. 相似文献
82.
Lucas Pailler Pascal Renard Edith Nicol Laurent Deguillaume Angelica Bianco 《Molecules (Basel, Switzerland)》2022,27(22)
FT-ICR MS (Fourier-transform ion cyclotron resonance mass spectrometry) analysis has shown great potential to aid in the understanding of the extremely high molecular diversity of cloud water samples. The main goal of this work was to determine the differences in terms of formula assignment for analytical (i.e., measurement replicates) and experimental replicates of a given cloud water sample. The experimental replicates, obtained by solid phase extraction, were also compared to the results obtained for freeze-dried samples to evaluate whether the presence of salts interferes with the analysis. Two S/N ratios, generally adopted for atmospheric samples, were evaluated, and three different algorithms were used for assignment: DataAnalysis 5.3 (Bruker), Composer (Sierra Analytics), and MFAssignR (Chemical Advanced Resolution Methods Lab). In contrast to other works, we wanted to treat this comparison from the point of view of users, who usually must deal with a simple list of m/z ratios and intensity with limited access to the mass spectrum characteristics. The aim of this study was to establish a methodology for the treatment of atmospheric aqueous samples in light of the comparison of three different software programs, to enhance the possibility of data comparison within samples. 相似文献
84.
白光再现激光全息照相的实验研究 总被引:1,自引:0,他引:1
以光的干涉和衍射为基础,讨论白光再现激光全息照相的原理,设计拍摄不同物体白光再现全息图的光路,探索能成功拍出白光再现反射全息图的条件和方法,并通过实验证明其可行性。 相似文献
85.
在Azpeitja对Taylor公式中Lagrange余项的"中间点"渐近性的研究基础上,又建立几个易于验证和推广的结果. 相似文献
86.
利用一维Cotes公式及高维积分Cartesian积空间上的求积法则,将Cotes公式推广到了n维空间上,并给出了简单的误差估计.该公式具有比文[4],[5],[6]相关结果更小的误差和更高的收敛阶等优点. 相似文献
88.
提出了一种利用解码技术对全息显微畸变图像进行校正的方法。把全息记录中的显微镜预放大过程作为物光波产生畸变的编码过程;在波前再现过程中,用全息记录中的预放大显微镜对再现的赝实像再次放大进行解码,从而获得清晰的全息显微图像。实验验证了该方法的有效性。 相似文献
89.
颜色视觉匹配中显示器颜色色差阈值的评价 总被引:9,自引:3,他引:6
为了对阴极射线管(Cathode ray tube,CRT)显示器颜色恰可辨别的小色差阈值进行评价,在中性灰背景下,用编制的颜色匹配程序,在CRT显示器上随机产生颜色,分别调节程序中R、G、B通道的值,使匹配色与随机色达到视觉上的一致,进行颜色视觉匹配实验,测量CRT显示器上随机色和匹配色的L*a*b*值.用不同的色差公式对实验数据分别从明度、色调、饱和度进行比较分析,色差公式评价小色差的性能是CIEDE2000优于CMC(2∶1)和CIEL*a*b*.在a*b*图上绘制实验颜色恰可辨别图.不同色差公式计算的CRT显示器恰可辨别小色差阈值不同. 相似文献
90.
The nervous system is a significant part of the human body, and peripheral nerve injury caused by trauma can cause various functional disorders. When the broken end defect is large and cannot be repaired by direct suture, small gap sutures of nerve conduits can effectively replace nerve transplantation and avoid the side effect of donor area disorders. There are many choices for nerve conduits, and natural materials and synthetic polymers have their advantages. Among them, the nerve scaffold should meet the requirements of good degradability, biocompatibility, promoting axon growth, supporting axon expansion and regeneration, and higher cell adhesion. Polymer biological scaffolds can change some shortcomings of raw materials by using electrospinning filling technology and surface modification technology to make them more suitable for nerve regeneration. Therefore, polymer scaffolds have a substantial prospect in the field of biomedicine in future. This paper reviews the application of nerve conduits in the field of repairing peripheral nerve injury, and we discuss the latest progress of materials and fabrication techniques of these polymer scaffolds. 相似文献