The High Performance Liquid Chromatography and Detection of Phospholipids and Triglycerides. III. Response of the Differential Refractive Index and Ultraviolet Absorption Detector

Abstract

A liquid chromatographic differential refractive index detector (RI) and an ultraviolet absorption (at 195 nm) detector (UV) were studied for detection of triglyceride (TG) and phospholipid (PL) molecular species. It was found that the log UV/RI detector response ratio was linearly related to the log of the number of conjugated double bonds (degree of unsaturation) of the lipid in a predictable fashion, indicating the usefulness of the combination of both detectors in TG and PL analysis. The detection limit of the RI detector for TG and PL was found to be 5 × 10 ^?7 M or as sensitive as the UV detector for saturated TG and PL.     

Bleach gel: a simple agarose gel for analyzing RNA quality

RNA-based applications requiring high-quality, non-degraded RNA are a foundational element of many research studies. As such, it is paramount that the integrity of experimental RNA is validated prior to cDNA synthesis or other downstream applications. In the absence of expensive equipment such as microfluidic electrophoretic devices, and as an alternative to the costly and time-consuming standard formaldehyde gel, RNA quality can be quickly analyzed by adding small amounts of commercial bleach to TAE buffer-based agarose gels prior to electrophoresis. In the presence of low concentrations of bleach, the secondary structure of RNA is denatured and potential contaminating RNases are destroyed. Because of this, the 'bleach gel' is a functional approach that addresses the need for an inexpensive and safe way to evaluate RNA integrity and will improve the ability of researchers to rapidly analyze RNA quality.     

Development and Validation of Derivative Spectrophotometric Method for Determination of Rabeprazole Sodium in Pharmaceutical Formulation

Abstract

The aim of this work is to develop and validate the derivative spectrophotometric method for determination of the proton pump inhibitor rabeprazole sodium in pharmaceutical formulations. The technique was applied using water (pH 10.0) as diluent. The first‐order derivative spectra were obtained at N=5, Δλ=4.0 nm, and determinations were made at 304 nm. The method showed high specificity in the presence of formulation excipients and good linearity in the concentration range of 6.0 to 18.0 µg/mL^?1. The intra‐ and interday precision data demonstrated the method has good reproducibility [Relative Standard Deviation ((RSD)=1.0 interdays)]. Accuracy was also evaluated and results were satisfactory (mean recovery of 99.15%). The detection and quantitation limits were 0.055 and 0.17 µg/mL^?1, respectively. The method was demonstrated to be adequate for routine analysis in quality control.     

Data Preprocessing for Chromatographic Fingerprint of Herbal Medicine with Chemometric Approaches

Abstract

Recently, the fingerprinting approach using chromatography has become one of the most potent tools for quality assessment of herbal medicine. Due to the complexity of the chromatographic fingerprint and the irreproducibility of chromatographic instruments and experimental conditions, several chemometric approaches such as variance analysis, peak alignment, correlation analysis, and pattern recognition were employed to deal with the chromatographic fingerprint in this work. To facilitate the data preprocessing, a software named Computer Aided Similarity Evaluation (CASE) was also developed. All programs of chemometric algorithms for CASE were coded in MATLAB5.3 based on Windows. Data loading, removing, cutting, smoothing, compressing, background and retention time shift correction, normalization, peak identification and matching, variation determination of common peaks/regions, similarity comparison, sample classification, and other data processes associated with the chromatographic fingerprint were investigated in this software. The case study of high pressure liquid chromatographic HPLC fingerprints of 50 Rhizoma chuanxiong samples from different sources demonstrated that the chemometric approaches investigated in this work were reliable and user friendly for data preprocessing of chromatographic fingerprints of herbal medicines for quality assessment.     

Validation of UV Spectrophotometric Method for Fexofenadine Hydrochloride in Pharmaceutical Formulations and Comparison with HPLC

Abstract

A simple, reproducible, accurate, and effective spectrophotometric method was developed and validated for the quantitation of the antihistamine fexofenadine in capsules and coated tablets. Ethanol was used as solvent and the absorbance at the wavelength of 220 nm was employed to the quantitation of the drug. The method validation was fulfilled through the evaluation of the analytical parameters of linearity, precision, accuracy, limits of detection, and quantitation and specificity. The method was linear (r=0.9999) at concentrations ranging from 8.0 to 20.0 µg ml^?1, precise (RSD intra‐day=0.29; 0.18; 0.39; RSD inter‐day=0.12 for capsules and RSD intra‐day=0.13; 0.16; 0.13; RSD inter‐day=0.13 for coated tablets), accurate (percentage recovery=99.97% for capsules and 100.51% for tablets), sensitive (limits of detection and quantitation of 0.10 and 0.29 µg ml^?1, respectively) and specific. The method was compared to a high performance liquid chromatography (HPLC) method, which was previously developed to the same drug. The results showed no significant difference between the methods in fexofenadine hydrochloride quantitation.     

基于期望视角的感知服务质量与顾客满意度区别研究

从期望出发,比较顾客视角的感知服务质量与满意度的区别。首先对期望概念进行明确界定,借鉴卡诺模型分类方法划分期望类别,基于重要性维度得到期望的三个类别:核心属性期望、重要属性期望和附加属性期望。以餐饮行业为实证研究背景,比较期望视角下的顾客满意度和感知服务质量的区别。发现核心属性期望、重要属性期望对顾客的服务质量评价影响更大,而附加属性期望对顾客的满意度评价影响更大。最后,给出企业对顾客期望、服务质量与顾客满意度的管理意义。     

信息与计算科学专业毕业设计的现状及改革研究

针对一般本科院校信息与计算科学专业毕业设计存在的问题,结合培养目标和专业特色,提出毕业设计改革思路.从选题、质量监控、过程管理、专业基础与内涵和指导教师队伍建设等方面实施相应的改革措施.     

爆破震动信号模极大值小波消噪方法的改进

针对模极大值小波消噪方法不利于所有信号特征的识别这一缺点,对该算法进行了改进,采用自适应的小波尺度来替换二进小波尺度,以更好地适应测试信号消噪的要求。应用改进前、后的消噪算法分别对实测爆破震动信号进行了分析,并将分析结果进行了比较。比较结果说明:改进后的算法消噪效果更好,消噪后信号同轴清晰、连续性好,信噪比由17.25提高到20.16,可以更好地实现爆破震动信号消噪的目的。     

往复式电镀金刚石线锯切割单晶硅片特性研究

通过往复式电镀金刚石线锯切割单晶硅片实验,分析了切片表面的微观形貌特点,研究了锯丝速度与进给速度对硅片的表面粗糙度、总厚度偏差(TTV)、翘曲度与亚表面损伤层厚度(SSD)的影响规律.结果表明:线锯锯切时材料以脆性模式去除,锯切表面的微观形貌呈现部分沟槽与断续划痕,并存在大量凹坑;锯丝速度增大,进给速度减小,表面粗糙度与SSD减小;锯丝速度增大,进给速度增大,硅片的翘曲度也随之增大;硅片TTV值与锯丝速度和进给速度的匹配关系相关.     

船用金属材料声发射信号特性研究

利用声发射技术对6种船用金属材料拉伸试验过程中产生的声发射信号进行了详细研究。通过对材料从弹性、屈服、强化直至破坏过程中的声发射信号参数统计分析,总结了船用金属材料拉伸过程中声发射信号事件数量、幅度、频率随拉力变化的分布规律,为在船用金属材料与结构缺陷中进行声发射检测的设备参数的配置、传感器的选择和危险等级的划分提供了基础数据。