气相色谱-质谱法测定蜡笔中的芳香伯胺

建立了固相萃取-气相色谱-质谱(SPE-GC-MS)检测儿童蜡笔中苯胺等9种芳香伯胺的方法.先用正己烷除去蜡笔中的烷烃类物质,再以甲醇为提取剂在室温下超声提取两次,提取液经过浓缩后与还原剂连二亚硫酸钠在70℃下反应30 min,将反应后的溶液通过硅藻土固相萃取柱净化收集,然后采用HP-5M色谱柱分离,并用质谱进行检测.采用该方法成功地实现了9种芳香伯胺的分离检测.对于不同的芳香伯胺的定量限为5 mg/kg,实际样品的平均回收率为86.02%～102.43%.实验结果证明,该方法准确、稳定,可以用于蜡笔中芳香伯胺的实际检验.     

Convenient Synthesis of Terminal Alkynes from anti‐3‐Aryl‐2,3‐dibromopropanoic Acids Using a K2CO3/DMSO System

A convenient, efficient, and generally applicable method was developed for the synthesis of terminal alkynes from anti‐3‐aryl‐2,3‐dibromopropanoic acids in the presence of DMSO and K₂CO₃.     

Esterification of levulinic acid to n-butyl levulinate over heteropolyacid supported on acid-treated clay

Levulinic acid has been identified as a promising green, biomass-derived platform chemical. n-Butyl levulinate is used as an important intermediate having diverse applications. The present work focuses on the synthesis of n-butyl levulinate by esterification of levulinic acid with n-butanol using heteropolyacid (HPA) supported on acid-treated clay montmorillonite (K10). 20% (w/w) dodecatungestophosphoric acid (DTPA) supported on K10 was found to be the most efficient catalyst with 97% levulinic acid conversion and 100% selectivity towards n-butyl levulinate. Effects of various process parameters were studied to examine the efficacy of 20% (w/w) DTPA/K10 for optimization of the activity.     

Synthesis and characterization of a 'fluorous' (fluorinated alkyl) affinity reagent that labels primary amine groups in proteins/peptides

Strong non-covalent interactions such as biotin-avidin affinity play critical roles in protein/peptide purification. A new type of 'fluorous' (fluorinated alkyl) affinity approach has gained popularity due especially to its low level of non-specific binding to proteins/peptides. We have developed a novel water-soluble fluorous labeling reagent that is reactive (via an active sulfo-N-hydroxylsuccinimidyl ester group) to primary amine groups in proteins/peptides. After fluorous affinity purification, the bulky fluorous tag moiety and the long oligoethylene glycol (OEG) spacer of this labeling reagent can be trimmed via the cleavage of an acid labile linker. Upon collision-induced dissociation, the labeled peptide ion yields a characteristic fragment that can be retrieved from the residual portion of the fluorous affinity tag, and this fragment ion can serve as a marker to indicate that the relevant peptide has been successfully labeled. As a proof of principle, the newly synthesized fluorous labeling reagent was evaluated for peptide/protein labeling ability in phosphate-buffered saline (PBS). Results show that both the aqueous environment protein/peptide labeling and the affinity enrichment/separation process were highly efficient.     

Finite Morse index solutions of an elliptic equation with supercritical exponent

We study the behavior of finite Morse index solutions of the equation

     

A unified Floquet theory for discrete, continuous, and hybrid periodic linear systems

In this paper, we study periodic linear systems on periodic time scales which include not only discrete and continuous dynamical systems but also systems with a mixture of discrete and continuous parts (e.g. hybrid dynamical systems). We develop a comprehensive Floquet theory including Lyapunov transformations and their various stability preserving properties, a unified Floquet theorem which establishes a canonical Floquet decomposition on time scales in terms of the generalized exponential function, and use these results to study homogeneous as well as nonhomogeneous periodic problems. Furthermore, we explore the connection between Floquet multipliers and Floquet exponents via monodromy operators in this general setting and establish a spectral mapping theorem on time scales. Finally, we show this unified Floquet theory has the desirable property that stability characteristics of the original system can be determined via placement of an associated (but time varying) system?s poles in the complex plane. We include several examples to show the utility of this theory.     

Homogenization limit of a parabolic equation with nonlinear boundary conditions

Consider the parabolic equation

(E)     

Periodic traveling curved fronts in reaction-diffusion equation with bistable time-periodic nonlinearity

This paper is concerned with the existence and stability of periodic traveling curved fronts for reaction-diffusion equations with time-periodic bistable nonlinearity in two-dimensional space. By constructing supersolution and subsolution, we prove the existence of periodic traveling wave fronts. Furthermore, we show that the front is globally stable.     

Superstability and rigorous asymptotics in singularly perturbed state-dependent delay-differential equations

We study the singularly perturbed state-dependent delay-differential equation

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On the number of limit cycles in small perturbations of a class of hyper-elliptic Hamiltonian systems with one nilpotent saddle

In this paper, we make a complete study on small perturbations of Hamiltonian vector field with a hyper-elliptic Hamiltonian of degree five, which is a Liénard system of the form x^′=y, y^′=Q₁(x)+εyQ₂(x) with Q₁ and Q₂ polynomials of degree respectively 4 and 3. It is shown that this system can undergo degenerated Hopf bifurcation and Poincaré bifurcation, which emerges at most three limit cycles in the plane for sufficiently small positive ε. And the limit cycles can encompass only an equilibrium inside, i.e. the configuration (3,0) of limit cycles can appear for some values of parameters, where (3,0) stands for three limit cycles surrounding an equilibrium and no limit cycles surrounding two equilibria.