Application of nanodiamonds in human body fluid analysis by matrix-assisted laser desorption/ionization mass spectrometry

Direct mass spectrometric analysis of complex biological samples is very important and challenging. In this paper, nanodiamonds have been successfully used in matrix-assisted laser desorption/ionization mass spectrometric analysis of human serum and urine. As a practical tool and platform, it can be widely used in the field of humoral proteomics, and it plays a very promising role in clinical diagnosis, including identification of novel disease-associated biomarkers.     

拉曼光谱在医学上的应用（Ⅰ）

本文叙述了最近拉曼光谱在生物医学应用上的技术进步，总结了拉曼光谱在生物医学上的应用，以及它在诊断和临床应用中的作用     

中红外光纤技术用于腮腺肿瘤诊断的研究

采用傅里叶变换红外光谱法与中红外光导纤维联用技术对１１例肿瘤，７例正常腮腺和１例舍格伦综合症的红外光谱进行了对比研究。结果发现正常腮腺和舍格伦综合症的红外光谱很相似，而与肿瘤的光谱存在着明显差别，同时也观察到良性与恶性肿瘤的光谱在若干波段处也有所不同．     

Laser absorption spectroscopy for combustion diagnosis in reactive flows: A review

Laser absorption spectroscopy (LAS) has been rapidly developed and widely applied to combustion diagnosis in recent decades. As a cost-effective tool for measuring multiple combustion parameters, LAS provides unique properties in terms of accuracy and sensitivity for understanding the reactions and kinetics in reactive flows. Line-of-sight and tomographic LAS techniques have stimulated numerous applications and been proved to be robust for in situ combustion diagnosis in uniform and non-uniform combustion fields, respectively. This review highlights the breakthroughs in the evolution of LAS techniques from the viewpoints of key principles, sensors and instrumentations developed for combustion diagnosis, with particular emphasis on a series of spatially-resolved LAS techniques with their recent applications on obtaining high-fidelity measurement results with minimal intrusion to the practical combustors. Along the way, we note some challenges and requirements for further development of the LAS-based combustion diagnosis.     

双狭缝相机对线状等离子体均匀性的监测

首次使用双狭缝相机对“神光”装置南路倍频线聚焦激光打靶所形成的线状等离子体均匀性进行了监测。由于实验条件的限制，相机的测量位置不太理想。为此，我们进行了修正，本文即是在修正基础上给出实验结果。修正后的结果表明，“神光”装置南路倍频线聚焦激光打靶部分发次所形成的线状等离子体均匀性不够理想。     

新型成像技术的实验系统研究

光学相干层析成像是一种新型成像技术。超辐射发光二极管和超短脉冲飞秒激光是能满足成像系统要求的理想光源。在开展光学相干层析相关技术研究的基础上 ,建立了国内第一台上述两种光源的成像实验研究装置 ,对系统的横向、纵向分辨率等基本性能进行了测定 ;并对多种实际样品进行了层析成像。     

新型光敏剂叶绿酸f对人膀胱癌T24细胞的光动力杀伤作用

将自主研发的新型光敏剂叶绿酸f与T24细胞共同孵育后,以650 nm激光照射,通过MTT法检测光动力处理后T24细胞的生长抑制率,流式细胞术检测凋亡情况,同时利用激光共聚焦显微镜488 nm和405 nm双波长激发,应用线粒体探针,进行叶绿酸f的亚细胞定位.结果显示叶绿酸f为浓度2.5,5,10 μg/mL对T24细胞生长抑制率在光能量密度4 J/cm2下为17.68％,49.35％和84.42％,在1 J/cm2时为4.34％,37.42％和78.38％;流式细胞术显示T24细胞光动力处理后,凋亡率为(45.23±1.2)％,显著高于对照组;激光共聚焦显微镜显示504 nm激发叶绿酸f发出的红光与488 nm激发线粒体探针产生的绿光分布基本一致.实验证明叶绿酸f对T24细胞杀伤效果明显,主要是通过作用于线粒体,诱导细胞凋亡来完成的.     

Polyurethane vascular prostheses in pigs

Microporous, compliant vascular prostheses made from segmented polyurethanes of various molecular stability, have been implanted in the infrarenal aorta of young pigs. Prostheses prepared from a hydrolytically suble polyetherurethane showed a limited patency up to 1 month. Composite prostheses with the wall made from a hydrolytically stable polyurethane and the lumen side prepared from a degradable polyurethane, were patent up to one year. A degradable layer of this composite prosthesis induced the growth of a neo-artery. Prostheses prepared from a degradable polyurethane alone, were substituted within 4 months with a functional neo-artery.Presented in part at the 17th International Biomaterials Symposium, San Diego, California, April 1985.     

A novel colorimetric PCR-based biosensor for detection and quantification of hepatitis B virus

Hepatitis B virus (HBV) can cause viral infection that attacks the liver and it is a major global health problem that put people at a high risk of death from cirrhosis of the liver and liver cancer. HBV has infected one third of the worldwide population, and 350 million people suffer from chronic HBV infection. For these reasons, development of an accurate, sensitive and expedient detection method for diagnosing, monitoring and assessing therapeutic response of HBV is very necessary and urgent for public health and disease control. Here we report a new strategy for detection of viral load quantitation of HBV based on colorimetric polymerase chain reaction (PCR) with DNAzyme-containing probe. The special DNAzyme adopting a G-quadruplex structure exhibited peroxidase-like activity in the presence of hemin to report colorimetric signal. This method has shown a broad range of linearity and high sensitivity. This study builds important foundation to achieve the specific and accurate detection level of HBV DNA with a low-cost and effective method in helping diagnosing, preventing and protecting human health form HBV generally all over the world and especially in developing countries.     

建立模糊诊断矩阵的一种新方法

本文首先提出了一个新的模糊逻辑算子——模糊加权综合算子“#”,进而给出建立模糊诊断矩阵的新方法——网络法 ,并给出了相应的算法