三基色白光LED的司辰节律因子研究

采用司辰节律因子模型,通过计算三基色白光LED光源在不同工作电流下的司辰节律因子,对可调色温的三基色白光LED光源进行非视觉效应研究。为了获取与自然光非视觉效应类似的LED白光,建立了司辰节律因子和相关色温分别相对于工作电流的关系模型,从而已知自然光的司辰节律因子和相关色温,就可以确定三基色LED的工作电流。通过测试一天内不同时刻的自然光光谱,根据上述模型推算出了三基色LED的工作电流。在所推算的三基色电流下,测试了白光LED光谱参数并计算了相应的司辰节律因子。与自然光司辰节律因子的对比结果表明,理论值和实验值的误差在1.1%以内,证实该方法具有可行性。本文所呈现的方法对于利用三基色白光LED模拟自然光具有一定的指导意义。     

多层对称薄膜的光子晶体光波导设计

基于多层对称薄膜的反射镜作用,设计了一种工作波长位于1550nm附近的基于周期性多层膜的光子晶体光波导。由平板波导的相关理论分析了波长在1515~1587nm范围内电磁波在波导中的传输性质。研究表明TE模和TM模在波导都是基模传输,其功率约束因子(Γ)限制在0.99~1之间,说明波导近乎完全将光波限制在其中传输。在控光能力上这种结构要比普通的三层薄膜波导更具优势。利用周期性多层膜的光子带隙结构,可以构造具有超低损耗的全向带隙介质光波导。     

Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis

An estimation method for determination of binding constants of receptors to ligands by affinity capillary electrophoresis was evaluated. On the basis of the theories of pseudostationary phase or so-called dynamic stationary phase, the retention factor (k) was used to represent the interaction between the receptor and ligand. k could be easily deduced from the migration times of the ligand and the receptor. Then, with the linear relationship of k versus the concentration of ligand in the running buffer, the binding constant K _b was calculated from the slope and intercept. In order to test its feasibility, the calculation method was demonstrated using three model systems: the interactions between vancomycin and N-acetyl-d-Ala-d-Ala, ristocetin and N-acetyl-d-Ala-d-Ala, and carbonic anhydrase B and an arylsulfonamide. Estimated binding constants were compared with those determined by other techniques. The results showed that this estimation method was reliable. This calculation method offers a simple and easy approach to estimating binding constants of ligands to receptors.     

Comparison of planar SDS-PAGE,CGE-on-a-chip,and MALDI-TOF mass spectrometry for analysis of the enzymatic de-<Emphasis Type="Italic">N</Emphasis>-glycosylation of antithrombin III and coagulation factor IX with PNGase F

Three different analytical techniques (planar SDS-PAGE, CGE-on-a-chip and MALDI-TOF-MS) applied for determination of the molecular weight of intact and partly and completely de-N-glycosylated human serum glycoproteins (antithrombin III and coagulation factor IX) have been compared. N-Glycans were removed from the protein backbone of both complex glycoproteins using PNGase F, which cleaves all types of asparagine-attached N-glycan provided the oligosaccharide has at least the length of a chitobiose core unit. Two of the applied techniques were based on gel electrophoretic separation in the liquid phase while the third technique was the gas-phase technique mass spectrometry. It was demonstrated that the enzymatic de-N-glycosylation generally worked well (completely or partially) with both glycoproteins (one containing only N-glycans and the second N- and O-glycans). All three methods were suitable for monitoring the de-N-glycosylation progress. While the molecular weights determined with MALDI-TOF-MS were most accurate, both gel electrophoretic methods provided molecular weights that were too high because of the attached glycan structures. Figure CGE-on-a-chip, SDS-PAGE and MALDI mass spectrometric pattern obtained from therapeutic glycoprotein     

Characterization of a putative Xylella fastidiosa diffusible signal factor by HRGC-EI-MS

Xylella fastidiosa (X.f.) is a plant pathogen with high levels of genomic similarity to Xanthomonas campestris pv. campestris (X.c.c.). It has been shown that X. fastidiosa synthesizes a putative diffusible signal factor (X.f.-DSF) that activates regulation of pathogenicity factor (rpf) genes in a X.c.c. reporter system, which might be involved in the regulation of pathogenesis associated genes as in X.c.c., as well as in quorum-sensing. The nature of the X.f.-DSF is not known, whereas the X.c.c.-DSF has been identified as cis-11-methyl-2-dodecenoic acid. In this work, the chemical nature of a putative X.f.-DSF molecule, able to restore endoglucanase activity in a X.c.c. rpfF mutant, was investigated as if it was a fatty acid derivative. Bioassays with X.c.c. reporter bacterium and X.f. culture extracts, based on endoglucanase restoration activity, were also carried out in order to confirm the DSFs molecules similarities. For this reason, a gas chromatography-mass spectrometry method was developed with standard fatty acids methyl esters mixtures. The retention time, as well as the fragmentation patterns, of each standard was used to identify the DSF molecule synthesized by X.f. in the culture medium. Typical ester fragmentation patterns (the derivatized analyte) were observed, such as: McLafferty rearrangement and migration of the Hdelta followed by 1,4-hydrogen shift and cleavage of the bond Cbeta-Cgamma, confirming the nature of this molecule. This confirmation was corroborated by the common peaks in both spectra. Besides, the observed retention time reinforces our conclusion since it corresponds to a methyl ester with 15 carbons. Since the X.f.-DSF molecule was tentatively identified as 12-methyl-tetradecanoic acid (by mass spectra library comparison), this standard compound was also analyzed, strongly suggesting that this is the identification of such a molecule. To our knowledge, this is the first time a DSF produced by X.f. has been characterized.     

How the interplay of different control mechanisms affects the initiator efficiency factor in controlled radical polymerization: An investigation using organometallic Mo-based catalysts

Compound CpMoI₂(iPr₂dad) (iPr₂dad = iPrNCHCHNiPr), obtained by halide exchange from CpMoCl₂(iPr₂dad) and NaI, has been isolated and characterized by EPR spectroscopy, cyclic voltammetry, and X-ray crystallography. Its action as a catalyst in atom transfer radical polymerization (ATRP) and as a spin trap in organometallic radical polymerization (OMRP) of styrene and methyl acrylate (MA) monomers has been investigated and compared with that of the dichloro analogue. Compound CpMoCl₂(iPr₂dad) catalyzes the ATRP of styrene and MA with low efficiency factors f (as low as 0.37 for MA and ethyl 2-chloropropionate as initiator), while it irreversibly traps the corresponding growing radical chains under OMRP conditions. On the other hand, compound CpMoI₂(iPr₂dad) has a greater ATRP catalytic activity than the dichloro analogue and yields f = 1 for MA and ethyl 2-iodopropionate as initiator. Under OMRP conditions, it does not irreversibly trap the growing radical chains. This comparison serves to illustrate the general principle that low initiator efficiency factors, sometimes observed in ATRP, may result from the interplay of the ATRP and OMRP mechanisms, when the latter ones involves an irreversible radical trapping process.     

Dynamic block GMRES: an iterative method for block linear systems

We present variants of the block-GMRES() algorithms due to Vital and the block-LGMRES(,) by Baker, Dennis and Jessup, obtained with replacing the standard QR factorization by a rank-revealing QR factorization in the Arnoldi process. The resulting algorithm allows for dynamic block deflation whenever there is a linear dependency between the Krylov vectors or the convergence of a right-hand-side occurs. implementations of the algorithms were tested on a number of test matrices and the results show that in some cases a substantial reduction of the execution time is obtained. Also a parallel implementation of our variant of the block-GMRES() algorithm, using and was tested on parallel computer, showing good parallel efficiency. This work was carried out while the author was at IM/UFRGS.     

合肥光源直流流强检测器系统的磁屏蔽

分析和测试了合肥光源杂散场对直流流强检测器系统的影响。给出了合肥光源ＤＣＣＴ系统的磁屏蔽的计算、设计和测量结果。屏蔽后,由杂散场引起的ＤＣＣＴ零漂由１ｍＡ降至１０μＡ以下。     

一类复代数方程组的高阶PCG法

对二维非线性Ｓｃｈｒｏｄｉｎｇｅｒ方程离散后的复代数方程组将高阶预处理技术与双ＣＧ法相结合给出高阶ＰＣＧ法,同时,将Ｍ阶复代数方程组化成２Ｍ阶非对称代数方程组,给出０阶、１阶和２阶近似ＬＵ分解的公式,并高阶ＰＣＧ法求解。计算结果表明,高阶ＰＣＧ法可以在０阶ＰＣＧ法的基础上将计算效率提高近一倍。