时间窗变动的车辆调度干扰管理模型与算法

针对客户时间窗变动对原物流配送车辆调度方案造成干扰的问题,运用干扰管理思想,分析干扰事件对路径、成本和服务时间三个方面的影响,对其干扰程度加以度量;以该干扰事件对原方案造成的广义费用偏离最小为目标,建立客户时间窗变动的干扰管理模型,通过判断客户时间窗变动对原方案是否产生影响进行干扰辨识,并基于该干扰辨识结果,设计基于禁忌搜索新的调度算法;算例不仅验证了模型和算法的有效性,而且,敏感性分析也验证了其对各种不同价值货物的适用性。实验结果表明,本文提出的干扰管理模型可以全面地刻画干扰对原方案的影响,干扰处理方法优于全局重调度方法,且能够在更短时间内生成满意的物流配送车辆调度调整方案。     

Synthetic control over the dynamics of mesoscaled cargo release from colloidal polymer vectors inside live cells

Directed delivery of mesoscaled cargo—for example, nanocrystals, proteins, or nucleic acids—to cells using polymer vectors impacts numerous biomedical fields. We introduce here the concept of dynamic complementarity as a simple, yet powerful approach to control the rate of mesoscaled cargo dissociation from colloidal polymer vectors once inside the cytosol. By tuning the degree of electrostatic reciprocity between the polymer vector and its cargo, it is possible to both deliver and release large cargo in live cells in a controllable manner over both long and short periods, pointing to a highly modular materials platform with molecularly tailored properties suited to task. © 2014 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem. 2015 , 53, 256–264     

Statistical optimization of cell disruption techniques for releasing intracellular X-prolyl dipeptidyl aminopeptidase from Lactococcus lactis spp. lactis

X-prolyl dipeptidyl aminopeptidase (PepX) is an intracellular enzyme from the Gram-positive bacterium Lactococcus lactis spp. lactis NRRL B-1821, and it has commercial importance. The objective of this study was to compare the effects of several cell disruption methods on the activity of PepX. Statistical optimization methods were performed for two cavitation methods, hydrodynamic (high-pressure homogenization) and acoustic (sonication), to determine the more appropriate disruption method. Two level factorial design (2FI), with the parameters of number of cycles and pressure, and Box–Behnken design (BBD), with the parameters of cycle, sonication time, and power, were used for the optimization of the high-pressure homogenization and sonication methods, respectively. In addition, disruption methods, consisting of lysozyme, bead milling, heat treatment, freeze–thawing, liquid nitrogen, ethylenediaminetetraacetic acid (EDTA), Triton-X, sodium dodecyl sulfate (SDS), chloroform, and antibiotics, were performed and compared with the high-pressure homogenization and sonication methods. The optimized values of high-pressure homogenization were one cycle at 130 MPa providing activity of 114.47 mU ml⁻¹, while sonication afforded an activity of 145.09 mU ml⁻¹ at 28 min with 91% power and three cycles.In conclusion, sonication was the more effective disruption method, and its optimal operation parameters were manifested for the release of intracellular enzyme from a L. lactis spp. lactis strain, which is a Gram-positive bacterium.     

Highly stereocontrolled and regiocontrolled syntheses of polyoxygenated [2.2.2]oxabicyclic synthons

Highly oxygenated cyclohexanes (known as cyclitols or carba-sugars) were synthesized regiospecifically and stereoselectively via a key inverse electron demand Diels–Alder cycloaddition of electron poor 2-pyrones with electron rich tert-butyldimethylsilyl vinyl ether. The resulting [2.2.2]oxabicyclic lactones proved to be versatile synthons; syn-dihydroxylations were achieved with very high stereocontrol, with up to five stereocenters being produced from commercially available or easily prepared planar starting materials.     

以商圈为中心的O2O动态外卖配送路径优化模型与算法

针对线上到线下(Online to Offline,O2O) 外卖路径优化问题,综合考虑其动态配送需求、货物区分等特点以及时间窗、载货量等约束条件,将商圈看作配送中心,将快递员数量与快递员总行驶时间作为最小化目标,提出了以商圈为中心的O2O动态外卖配送路径优化模型。采用周期性处理新订单的方法将相应的快递员路径的动态调整问题转化为一系列静态TSP子问题,设计了一种分阶段启发式实时配送路径优化算法框架,并给出了一个具体算法和一个数值计算实例。在VRP通用算例的基础上,以商圈为中心生成测试算例,对本文算法进行仿真实验,并与其他算法比较。结果表明：本文算法能充分利用新订单附近的快递员进行配送,并优化其配送路径,有效减少了快递员数量与快递员总行驶时间。     

路段容量约束弹性需求交通均衡分配近似算法

本文研究了带路段容量约束弹性需求用户均衡交通分配问题及其近似解法.采用超需求模型将弹性需求转化为固定需求,提出了一种带路段容量约束弹性需求用户均衡交通分配近似算法.该算法在迭代过程中,通过不断自适应调节排队延误因子、误差因子来近似真实路段行驶时间,使路段流量逐步满足约束条件,最终达到广义用户均衡.这种方法克服了容量约束弹性需求用户均衡分配计算量大及随机分配法要求枚举所有路径的困难.随后证明了算法的收敛性,并对一个小型路网进行了数值试验.     

Photoelectro-synergistic catalysis combined with a FIA system application on determination of chemical oxygen demand

In this paper, photoelectro-synergistic catalysis oxidation of organics in the water on Ti/TiO₂/PbO₂ electrode was investigated. The prepared TiO₂ film was investigated with Atomic force micrograph (AFM). Furthermore, the results were compared with those obtained from electrocatalysis (EC) and electro-assisted photocatalysis (PC). The method proposed employed photoelectro-synergistic catalysis (PEC), together with flow injection analysis, to determine the chemical oxygen demand (COD) values. It was shown that the method of photoelectro-synergistic catalysis had lower detection limit (15.0 mg l⁻¹) and wider linear range (30.0–2500.0 mg l⁻¹) than the methods of electro-assisted photocatalysis and electrocatalysis. The results obtained by the proposed method and conventional one were compared by carrying out the experiment on 20 wastewater samples and also agreed well by high correlation (R = 0.9912).     

风险中性零售商的合作广告策略与订货模型

考虑了由一个制造商与一个零售商构成的单期二阶段供应链是否进行合作广告的博弈问题.面对市场需求的不确定性,零售商从制造商处订购报童类型产品销售给消费者,零售商具有风险中性的行为特征.通过不合作广告与合作广告两种情形,制造商与零售商进Stackelberg主从博弈,得到了均衡解,比较后发现,合作广告下的最优解及利润总是优于不合作广告下的最优解和利润,告诉了上下游企业采用合作广告的广告策略.最后,通过数值算例,给出了需求敏感系数对最优决策的影响,同时也论证了有关结论.     

灰色系统分析应用——预测上海房地产市场需求量变化并对其影响因素做关联度分析

近两年上海房地产市场快速发展 ,主要利用灰色分析法预测未来几年上海房地产市场的需求量的增长情况并对市场需求的主要影响因素做出关联度分析 ,最后得出结论 .     

随机需求下多供应商和多零售商的生产-库存-运输联合优化模型

考虑随机需求下多供应商和多零售商的生产-库存-运输联合优化问题.在联合优化时,首先利用最近邻算法将各零售商分成不同区域,分区后问题转化为随机需求下单供应商对多零售商的生产-库存-运输联合优化问题.在每个分区内,由供应商统一决策其分区内各零售商的送货量和送货时间.利用粒子群算法和模拟退火算法相结合的两阶段算法求出最优送货量、最优运输路径和最大期望总利润.然后采用收入共享契约将增加的利润合理分配给各供应商和各零售商,使各方利润都得到增加,从而促使各方愿意合作.通过数值算例验证了联合优化模型优于独立决策模型.