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51.
Patricia M. G. Paiva Luana C. B. B. Coelho 《Applied biochemistry and biotechnology》1992,36(2):113-118
Two additional electrophoretically distinct molecular forms, isoforms (iso) 2 and 3, with lectin properties were isolated
fromCratylia mollis Mart, seeds (FABACEAE), by extraction with 0.15M NaCl and ammonium sulfate fractionation, followed by chromatography on Sephadex
G-75 and Bio-Gel P-200 (iso 2), as well as CM-Cellulose and Sephadex G-75 (iso 3). Both isoforms were human group nonspecific
and showed distinct specificity. Polyacrylamide gel electrophoresis resolved iso 2 and 3 in polypeptides of apparent mol wts
60 and 31 kDa, respectively; a distinct isoelectric focusing pattern was obtained for iso 2 and 3, under denaturing and reducing
conditions. 相似文献
52.
53.
S. L. Reindl G. M. Pastor 《Zeitschrift für Physik D Atoms, Molecules and Clusters》1993,26(Z1):171-173
The low-energy excitation spectra of metal dimers are determined by solving exactly a realistic many-body Hamiltonian with inter- and intra-atomic Coulomb interactions. Our results for Cu2 and Ag2 are in very good agreement with the excitation energies derived from recent photodetachment experiments. The characteristics of the many-body excited states in these clusters are briefly discussed. 相似文献
54.
The effect of the trace metals Cu, K, Na, and Ca, separately or in mixture, on fermentation time, ethanol production rate,
and cell growth in the fermentation of synthetic media containing sucrose is discussed. The results are related to the range
of contents found in raw materials, molasses and raisins, in order to determine their optimum concentrations for alcohol production. 相似文献
55.
Monot Frédéric Benoit Yves Ballerini Daniel Vandecasteele Jean-Paul 《Applied biochemistry and biotechnology》1990,(1):375-386
Utilization of lipases for synthesis of esters of hydrophilic polyols has been investigated. The choice of a suitable solvent
is crucial in this type of reaction. An interesting case is fatty acid esters from neopentylpolyols, such as trimethylolpropane,
which are of great interest as high temperature lubricants. Enzymatic synthesis of trimethylolpropane tricaprylate was studied
as an alternative to chemical manufacturing. Triester production occurred only if the water produced by esterification was
continuously removed from the medium. In these condition, kinetics of appearance and transformation of mono-, di- and triesters
were determined in order to define optimal conditions. 相似文献
56.
Shetty Kalidas Korus roger A. Crawford Dom L. 《Applied biochemistry and biotechnology》1989,(1):825-843
Glycine max was used as a model plant cell suspension culture to establish relationships among growth kinetics, phenolics production,
elicitor action, and peroxidase activity. Timing of elictor addition through monitoring of peroxidase provided an excellent
means of optimizing yields of phenolics and reduced the time span during which phenolics were formed, negating the need for
a secondary production medium. We have also determined that calcium and other cellular effectors like polyamines and organic
osmolytes, when used in conjunction with elicitors, enhance phenolics production. Calcium directly enhanced elicitation, whereas
polyamines and other osmolytes such as glycerol and proline extended cell viability. The study also demonstrated potential
for enhancing secondary metabolite production by a combination of elicitation, cell viability stabilizers, and by addition
of nutrients at the time of elicitation. 相似文献
57.
Rivard C. J. Himmel M. E. Vinzant T. B. Adney W. S. Wyman C. E. Grohmann K. 《Applied biochemistry and biotechnology》1989,(1):461-478
Economic evaluations of the capital costs for anaerobic digestion systems for gas production show that the reactor is a significant
cost component. The successful application of high solids digestion of processed MSW (e.g., greater than 10% solids within
the digester) would allow a decrease in reactor volume with maintenance of relatively high gas production rates. However,
high solids slurries do not mix well in conventional stirred tank reactors. A horizontal shaft, hydraulically driven reactor
was designed and fabricated to test the anaerobic digestion of high solids concentrations. Digester performance was evaluated
as a function of experimental parameters such as nutrient requirements, feeding rates, pH control, and agitator design/ rotation
speed; horsepower of mixing was also evaluated for the reactor. Several startup protocols were examined to obtain a biologically
stable anaerobic fermentation at high solids levels. 相似文献
58.
Lamb pregastric lipase was purified from a commercial source using delipidation, solubilization with KSCN, acid-precipitation, pepsin-digestion, affinity chromatography with agarose-Cibacron Blue F3GA, gel filtration, and elution from a native 10% (w/v) polyacrylamide gel. The enzyme had a single subunit of 68,000 Da with maximum esterase activity when measured at pH 6.0 and 30 degrees C. The enzyme preferentially hydrolyzed short- and medium-chain (C4, C6, and C8) synthetic esters and short-chain (C4 and C6) monoacid triglycerides. The NH2-terminal sequence demonstrated high homology with gastric and lingual lipases. 相似文献
59.
Nidetzky Bernd Griessler Richard Weinhausel Andreas Haltrich Dietmar Kulbe Klaus D. 《Applied biochemistry and biotechnology》1997,(1):159-172
Some important process properties of α-l,4-D-ghican phosphorylases isolated from the bacteriumCorynebacterium callunae and potato tubers (Solatium tuberosum) were compared. Apart from minor differences in their stability and specificity (represented by the maximum degree of maltodextrin
conversion) and a 10-fold higher affinity of the plant phosphorylase for maltodextrin (K
M of 1.3 g/L at 300 mM of orthophosphate), the performances of both enzymes in a continuous ultrafiltration membrane reactor
were almost identical. Product synthesis was carried out over a time course of 300–400 h in the presence or absence of auxiliary
pullulanase (increasing the accessibility of the glucan substrate for phosphorolytic attack up to 15–20%). The effect of varied
dilution rate and reaction temperature on the resulting productivities was quantitated, and a maximum operational temperature
of 40°C was identified. 相似文献
60.
{(MesGa)3[GaP(H)Mes](PMes)4}, a Phosphorus-substituted Ga? P-Heterocubane A mixture of MesGaCl2/GaCl3 (ratio 3:1) reacts with 5 equivalents of MesPLi2 in THF at ?78°C to the title compound {(MesGa)3[GaP(H)Mes](PMes)4} ( 1 ) by use of the “dilution principle”. 1 can be obtained in 30% yield. Recrystallization of 1 from DME and toluene, respectively, gives 1 · 0.5 DME and 1 · toluene. 1 was characterized by NMR-, IR-, and MS-techniques. According to the X-ray structure determination of 1 · toluene, 1 has a heterocubane structure, one corner of which is substituted with an P(H)Mes group. 相似文献