仪器车减震台的设计优化

本文介绍一种以塑性变形减震概念设计的用于地下爆炸地表层裂区内的仪器车减震台。文中叙述了设计的原理和计算方法。通过同普通的由弹簧和阻尼器构成的减震台的计算对比,说明这种以塑性变形减震概念设计的减震台具有许多优点。对减震台做了吊高达1.5m的落地冲击实验,其结果同计算一致。     

基于CPCI总线的雷达数据采集与控制系统显控软件设计

CPCI总线作为PCI总线的加固版本,目前已成为计算机上流行的高速外设接口总线。在工业自动化领域,进行数据采集和控制基本上也都是通过这一成熟技术来实现的。本文简要介绍该测量雷达和数据采集与控制系统的基本结构,以及CPCI总线标准,重点分析显控软件的功能需求,采用自顶向下的设计,并在VC环境下基于MFC架构利用多线程和双缓存技术以及多种ActiveX控件进行显控软件设计。实现了对测量雷达的控制以及对雷达回波的原始数据的实时存取和绘制。     

Nonlinear stability of traveling wave solutions for the compressible fluid models of Korteweg type

This paper is concerned with the existence and time-asymptotic nonlinear stability of traveling wave solutions to the Cauchy problem of the one-dimensional compressible fluid models of Korteweg type, which governs the motions of the compressible fluids with internal capillarity. The existence of traveling wave solutions is obtained by the phase plane analysis, then the traveling wave solution is shown to be asymptotically stable by the elementary L²$L^2$-energy method.     

Absolute quantification of superoxide dismutase in cytosol and mitochondria of mice hepatic cells exposed to mercury by a novel metallomic approach

In the last years, the development of new methods for analyzing accurate and precise individual metalloproteins is of increasing importance, since numerous metalloproteins are excellent biomarkers of oxidative stress and diseases. In that way, methods based on the use of post column isotopic dilution analysis (IDA) or enriched protein standards are required to obtain a sufficient degree of accuracy, precision and high limits of detection. This paper reports the identification and absolute quantification of Cu,Zn-superoxide dismutase (Cu,Zn-SOD) in cytosol and mitochondria from mice hepatic cells using a innovative column switching analytical approach. The method consisted of orthogonal chromatographic systems coupled to inductively coupling plasma-mass spectrometry equipped with a octopole reaction systems (ICP-ORS-MS) and UV detectors: size exclusion fractionation (SEC) of the cytosolic and mitochondrial extracts followed by online anion exchange chromatographic (AEC) separation of Cu/Zn containing species. After purification, Cu,Zn-SOD was identified after tryptic digestion by molecular mass spectrometry (MS). The MS/MS spectrum of a doubly charged peptide was used to obtain the sequence of the protein using the MASCOT searching engine. This optimized methodology reduces the time of analysis and avoids the use of sample preconcentration and clean-up procedures, such as cut-off centrifuged filters, solid phase extraction (SPE), precipitation procedures, off-line fractions insolates, etc. In this sense, the method is robust, reliable and fast with typical chromatographic run time less than 20 min. Precision in terms of relative standard deviation (n = 5) is of 3–5% and detection limits is 0.21 ng Cu g⁻¹.     

Analysis of oligonucleotides by hydrophilic interaction liquid chromatography coupled to negative ion electrospray ionization mass spectrometry

Hydrophilic interaction liquid chromatography (HILIC) is here successfully coupled to negative-ion electrospray ionization time-of-flight mass spectrometry (ESI-TOFMS) for the analysis of synthetic and chemically modified oligonucleotides. Separation was performed on a 2.1 mm × 100 mm PEEK ZIC^® HILIC column packed with hydrophilic stationary phase with a permanent zwitterionic functional group and a particle size of 3.5 μm with an average pore diameter of 200 Å. A method was developed to separate homogeneous and heterogeneous oligonucleotides as well as methylated oligonucleotides using a quaternary pumping system containing ammonium acetate and water with an acetonitrile gradient. Analyses of oligonucleotides were performed by LC/MS with a detection limit of 2.5 picomole (20 mer) with signal to noise ratio (S/N) of 4.12. The influence of the eluent composition, type of buffer and its concentration, and organic modifier were also evaluated. The HILIC LC/MS method presented in this paper used common, ‘MS friendly’, mobile phases achieving sensitive and selective oligonucleotide analysis.     

非晶硅界面缓冲层对非晶硅锗电池性能的影响

针对非晶硅锗电池本征层高锗含量时界面带隙失配以及高界面缺陷密度造成电池开路电压和填充因子下降的问题,通过在PI界面插入具有合适带隙的非晶硅缓冲层,不仅有效缓和了带隙失配,降低界面复合,同时也通过降低界面缺陷密度改善内建电场分布,从而提高了电池的收集效率. 进一步引入IN界面缓冲层以及对非晶硅锗本征层进行能带梯度设计,在仅采用Al背电极时,单结非晶硅锗电池转换效率达8.72%. 关键词： 非晶硅缓冲层 非晶硅锗薄膜太阳电池 带隙 界面     

WIN95下DirectX的动画设计技术

利用DirectDraw模块进行快速动画设计，重点对其直接操纵显存的特性和用后缓冲区实现动画中背景擦除和重绘的技巧进行探索，并结合火焰字的实例说明本文提出的方法是Win95下开发动画软件的一种切实可行的方法．