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31.
通过PCR扩增出猪传染性胃肠炎病毒(TGEV)聚合酶部分片段,反向插入逆转录病毒表达载体pLXSN中,用脂质体法将重组质粒plxas-pol转染PA317细胞,经抗生素G418筛选出稳定的产毒细胞克隆,分别扩大培养后,取其上清液感染小鼠成纤维细胞NIH3T3,细胞克隆产生的重组病毒效价达9.0×105CFU/mL。用高效价假病毒感染IBRS2细胞,再经抗生素G418筛选,提取细胞克隆总RNA,经RT-PCR证明plxas-pol整合入IBRS2细胞。以TGEV感染IBRS2细胞和具有抗性的IBRS2细胞所产生的细胞病变为指标,证明该反义RNA对病毒有明显抑制作用,抑制率约为80%。用2×103和4×102TCID50/mL剂量的TGEV感染时,引起细胞死亡的时间分别为21 h和27 h,与对照组相比,抗性细胞系可明显延迟因病毒感染引起细胞死亡的时间。 相似文献
32.
Huseyin Ince 《Computational Management Science》2006,3(2):161-174
The nature of the financial time series is complex, continuous interchange of stochastic and deterministic regimes. Therefore,
it is difficult to forecast with parametric techniques. Instead of parametric models, we propose three techniques and compare
with each other. Neural networks and support vector regression (SVR) are two universally approximators. They are data-driven
non parametric models. ARCH/GARCH models are also investigated. Our assumption is that the future value of Istanbul Stock
Exchange 100 index daily return depends on the financial indicators although there is no known parametric model to explain
this relationship. This relationship comes from the technical analysis. Comparison shows that the multi layer perceptron networks
overperform the SVR and time series model (GARCH). 相似文献
33.
Zuo-liang Xu 《应用数学学报(英文版)》2007,23(4):629-636
In this paper,we study mixed elastico-plasticity problems in which part of the boundary is known,while the other part of the boundary is unknown and is a free boundary.Under certain conditions,this problemcan be transformed into a Riemann-Hilbert boundary value problem for analytic functions and a mixed boundaryvalue problem for complex equations.Using the theory of generalized analytic functions,the solvability of theproblem is discussed. 相似文献
34.
On interpolatory divergence-free wavelets 总被引:1,自引:0,他引:1
We construct interpolating divergence-free multiwavelets based on cubic Hermite splines. We give characterizations of the relevant function spaces and indicate their use for analyzing experimental data of incompressible flow fields. We also show that the standard interpolatory wavelets, based on the Deslauriers-Dubuc interpolatory scheme or on interpolatory splines, cannot be used to construct compactly supported divergence-free interpolatory wavelets.
35.
José L. Ló pez Nico M. Temme 《Transactions of the American Mathematical Society》2004,356(11):4323-4342
Taylor expansions of analytic functions are considered with respect to several points, allowing confluence of any of them. Cauchy-type formulas are given for coefficients and remainders in the expansions, and the regions of convergence are indicated. It is explained how these expansions can be used in deriving uniform asymptotic expansions of integrals. The method is also used for obtaining Laurent expansions in several points as well as Taylor-Laurent expansions.
36.
Jin Cheng Ching-Lung Lin Gen Nakamura 《Proceedings of the American Mathematical Society》2005,133(8):2359-2367
In this paper we prove the following kind of unique continuation property. That is, the zero on each geodesic of the solution in a real analytic hypersurface for second order anisotropic hyperbolic systems with real analytic coefficients can be continued along this curve.
37.
Howard Jacobowitz Gerardo Mendoza 《Transactions of the American Mathematical Society》2003,355(10):4201-4222
We study embeddings of complex vector bundles, especially line bundles, in the complexification of the tangent bundle of a manifold. The aim is to understand implications of properties of interest in partial differential equations.
38.
This paper considers a new approach to a priori sparsification of the sparsity pattern of the factorized approximate inverses (FSAI) preconditioner using the so‐called vector aggregation technique. The suggested approach consists in construction of the FSAI preconditioner to the aggregated matrix with a prescribed sparsity pattern. Then small entries of the computed ‘aggregated’ FSAI preconditioning matrix are dropped, and the resulting pointwise sparsity pattern is used to construct the low‐density block sparsity pattern of the FSAI preconditioning matrix to the original matrix. This approach allows to minimize (sometimes significantly) the construction costs of low‐density high‐quality FSAI preconditioners. Numerical results with sample matrices from structural mechanics and thin shell problems are presented. Copyright © 2002 John Wiley & Sons, Ltd. 相似文献
39.
马科维兹资产组合选择模型的旋转算法 总被引:2,自引:0,他引:2
提出线性不等式组的一种旋转算法,并用其求解马科维兹资产组合选择模型,此算法每次迭代约需n^2次乘法和加法,其中n是模型中变量的数目,在微机上运行Delphi程序的实验结果表明,从上海和深圳股市1072支股票70期周末收盘价计算出20个最优投资组合仅需314次迭代和45s。 相似文献
40.