A Stability-Indicating Spectrophotometric Method for the Determination of Fenoterol in Pharmaceutical Preparations.

ABSTRACT

A simple and highly sensitive spectrophotometric method was developed for the determination of fenoterol hydrobromide in pharmaceutical formulations. The method is based on measurement of the orange-yellow color peaking at 436 nm, produced when the drug is coupled with diazotized benzocaine in triethylamine medium. The method is applicable over the range of 0.5 – 10 μg/ml, with minimum detectability of 3.6 ng/ml (≈ 9.4×10^?9 M). The molar absorptivity is 4.61×10⁴ L. mol^?1. cm^?1. with relative standard error of 0.196%. The proposed method was successfully applied to the determination of fenoterol in its dosage forms; the mean recoveries of 99.61 ± 1.59 and 98.97 ± 1.52 for tablets and aerosols, respectively, were obtained. The results of the proposed method were favorably compared to those obtained by the official and reference methods. No i8543168 was encountered from the co-formulated drug ipratropium bromide, common excipients and alkaline induced degradation product. A proposal of the reaction pathway is presented.     

Spectrophotometric and Spectrofluorometric Determination of Heptaminol and mexiletine in Their Dosage Forms

Abstract

Two simple, rapid specific methods were developed for the determination of heptaminol and mexiletine and their dosage forms. The methods are based on the reaction of either heptaminol or mexiletine with acetylacetone-formaldehyde reagent to give a yellow chromophore measurable spectrophotometrically at 344 or 338 nm or flurometrically at 480 nm for heptaminol and mexiletine, respectively. The color was stable for at least 1/2 h. Beer's law was valid within a concentration range of 15–30 and 8–20 μg ml^?1 spectrophotometrically and 0.2–0.8 and 0.4–1.0 μg ml^?1 fluorometrically for heptaminol and mexiletine, respectively.     

The establishment and evaluation of near infrared universal model to determinate the effective ingredient content in pesticide rapidly

A near infrared universal quantitative analysis model was established to determinate the effective ingredient content in pesticide EC(hikemalisation) by the PLS(partial least squares) algorithm,the model predictive ability was evaluated by the external inspection method.The model was established among samples containing the same active ingredient from five different companies, and the model determination coefficient R~2 and RMSECV(root mean square error of cross validation) were 0.9997 and 0.0223, respectively,the relative error between predicted value and chemical value of the testing set samples was between—2.71%and 3.36%,which indicated that the method to determinate the effective ingredient content in pesticide EC by the established universal model can meet the need of pesticide market monitoring.     

Inequalities on the inner radius of univalency and the norm of pre-Schwarzian derivative

In this paper, we get a lower bound of inner radius of univalency of Schwarzian derivative by means of the norm of pre-Schwarzian derivative. Furthermore, we apply the theory of Universal Teichmuller Space to explain its geometric meaning which shows the relationship between the inner radius in Universal Teichmuller Space embedded by Schwarzian derivative and the norm defined in Universal Teichmuller Space embedded by pre-Schwarzian derivative.     

Ion-pairing and reversed phase liquid chromatography for the determination of three different quinolones: Enrofloxacin,lomefloxacin and ofloxacin

Two simple and sensitive high performance liquid chromatographic (HPLC) methods have been developed for the simultaneous determination of three different quinolones: enrofloxacin, lomefloxacin and ofloxacin in their pure and dosage forms, one with reversed phase HPLC and the other with ion-pair HPLC. In reversed phase HPLC, method (A), the mobile phase consists of 2.18% aqueous solution of KH₂PO₄ with pH adjusted to 2.4 ± 0.2 with acetonitrile (80:20; v/v), the mobile phase pumped at flow rate of 1.2 ml min^?1. A Neucleosil C₁₈ column (10 μm, 100 Å), 250 mm length × 4.6 mm diameter was utilized as stationary phase. Detection was affected spectrophotometrically at 294 nm. While in ion-pair HPLC, method (B), the mobile phase was aqueous solution of 0.65% sodium perchlorate and 0.31% ammonium acetate adjusted to pH 2.2 ± 0.2 with orthophosphoric acid: acetonitrile (81:19; v/v), the mobile phase pumped at flow rate of 1.5 ml min^?1. A μ bondapack C₁₈ column (10 μm, 100 Å), 250 mm length × 4.6 mm diameter was utilized as stationary phase. Detection was affected spectrophotometrically at 294 nm. Linearity ranges for enrofloxacin, lomefloxacin and ofloxacin were 4.0–108, 7.0–112 and 8.0–113 μg ml^?1, respectively using method A and 8.0–112, 7.0–112 and 5.0–105 μg ml^?1, respectively applying method B. Minimum detection limits obtained were 0.013, 0.023 and 0.035 μg ml^?1 for enrofloxacin, lomefloxacin and ofloxacin, respectively using method A, and 0.028, 0.023 and 0.011 μg ml^?1 using method B. The proposed methods were further applied to the analysis of enrofloxacin in injection and tablets containing the ofloxacin and lomefloxacin drugs, and the results were satisfied.     

Kinetic spectrophotometric determination of pravastatin in drug formulations via derivatization with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl)

A simple and sensitive kinetic spectrophotometric method for the quantitative analysis of pravastatin sodium (PVS) in pure and pharmaceutical formulations has been described. The method is based on the formation of colored product between PVS and 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in acetone medium at 55 ± 2 °C. The reaction is followed spectrophotometrically by measuring the increase in absorbance at 462 nm as a function of time. The initial rate and fixed time methods were adopted for constructing the calibration curves. The linearity ranges were found to be 15.0–50.0 and 10.0–70.0 μg mL^?1 for initial rate and fixed time methods, respectively. The limits of detection for initial rate and fixed time methods are 0.029 and 0.086 μg mL^?1, respectively. Both methods have been applied successfully for the estimation of PVS in commercial dosage forms with no interference from the excipients. The results are compared with the HPLC pharmacopoeial method.     

Dirichlet series of Rankin-Cohen brackets

Given modular forms f and g of weights k and ?, respectively, their Rankin-Cohen bracket corresponding to a nonnegative integer n is a modular form of weight k+?+2n, and it is given as a linear combination of the products of the form f(r)g(n−r) for 0?r?n. We use a correspondence between quasimodular forms and sequences of modular forms to express the Dirichlet series of a product of derivatives of modular forms as a linear combination of the Dirichlet series of Rankin-Cohen brackets.     

Beyond endoscopy for the Rankin-Selberg L-function

We try to understand the poles of L-functions via taking a limit in a trace formula. This technique avoids endoscopic and Kim-Shahidi methods. In particular, we investigate the poles of the Rankin-Selberg L-function. Using analytic number theory techniques to take this limit, we essentially get a new proof of the analyticity of the Rankin-Selberg L-function at s=1. Along the way we discover the convolution operation for Bessel transforms.     

The effect of torsion on the distribution of Ш among quadratic twists of an elliptic curve

Let E be an elliptic curve of rank zero defined over Q and ? an odd prime number. For E of prime conductor N, in Quattrini (2006) [Qua06], we remarked that when ?||ETor(Q)|, there is a congruence modulo ? among a modular form of weight 3/2 corresponding to E and an Eisenstein series. In this work we relate this congruence in weight 3/2, to a well-known one occurring in weight 2, which arises when E(Q) has an ? torsion point. For N prime, we prove that this last congruence can be lifted to one involving eigenvectors of Brandt matrices Bp(N) in the quaternion algebra ramified at N and infinity. From this follows the congruence in weight 3/2. For N square free we conjecture on the coefficients of a weight 3/2 Cohen-Eisenstein series of level N, which we expect to be congruent to the weight 3/2 modular form corresponding to E.     

A new zero-density result of <Emphasis Type="Italic">L</Emphasis>-functions attached to Maass forms

Let f denote a normalized Maass cusp form for SL(2, ℤ), which is an eigenfunction of all the Hecke operators T(n) as well as the reflection operator $ T_{ - 1} :z \to - \bar z $ T_{ - 1} :z \to - \bar z . We obtain a zero-density result of the L-function attached to f near σ = 1. This improves substantially the previous results in this direction.