马来酸酐与1，2－环己烷二羧酸二异丁酯的气液平衡

采用小沸点仪测定了马来酸酐和1,2－环己烷二羧酸二异丁酯二元系统在不同配料组成条件下的温度与压力数据，并通过内插法得到不同压力下的温度与配料组成的数据.进一步采用单纯形法优化NRTL方程参数，推算了平衡气液相组成，发现该二元系统具有最高压力恒沸点，且该恒沸点随压力的变化显著.当压力降低至20 kPa时, 由温度随气相组成的变化趋势知该恒沸点将趋于消失.     

Synthesis of novel derivatives of 2-(azolylimino)thiazoline

Successive alkylation of 5-(3-phenylthioureido)-3H-imidazole-4-carboxamides with alkyl halides and chloroacetone gave (N-oxopropylimidazolyl)isothioureas, which were easily converted into derivatives of purine and imidazopyrazinone. In the case of ethyl 5-(3-phenylthioureido)-3H-imidazole-4-carboxylate, primary alkylation occurs at the N atom of the imidazole ring. Reactions of 5-(3-phenylthioureido)-3H-imidazole-4-carboxamides with haloketones afforded a number of 4-hydroxy-2-imidazolyliminothiazolidines and 2-imidazolylimino-Δ⁴-thiazolines.__________Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 10, pp. 2196–2204, October, 2004.     

Titanocene(III) mediated radical cyclization of α-bromo carbonyl compounds: synthesis of tri-substituted tetrahydrofurans

A novel and efficient methodology has been developed for the construction of synthetically important tri-substituted tetrahydrofuran derivatives from bromo-alkenes and bromo-alkynes by radical cyclization reactions using the radical initiator Cp₂TiCl, generated in situ from commercially available titanocene dichloride and Zn dust in tetrahydrofuran under argon.     

Effect of arrangement of the styryl fragment on the optical properties and complexation of mono-and bis(styryl)-substituted <Emphasis Type="Italic">N</Emphasis>-methylpyridinium perchlorates containing benzo-15-crown-5 ether moieties

Using ¹H NMR spectroscopy and steady-state and time-resolved electronic spectroscopy, the optical properties of mono-and bis(styryl)pyridinium perchlorates and their complexes with Mg²⁺, Ba²⁺ cations were studied. The stability constants of the complexes were determined using spectrophotometric titration. The formation of inclusion complexes for Mg²⁺ and sandwich type complexes for Ba²⁺ results in fluorescence enhancement and increases the lifetimes of the excited states of the initial bis-styryl ligands. The variation of position of the styryl fragment in the pyridinium aromatic ring gives rise to photochromic crown ethers with different optical and photophysical characteristics and is also an easy route to bis(crown-ethers) of symmetrical and unsymmetrical structure. Published in Russian in Izvestiya Akademii Nauk. Seriya Khimicheskaya, No. 11, pp. 2092–2100, November, 2007.     

Lysophosphatidic acid induces astrocyte proliferation in hippocampus slices partially through activating extracellular signal-regulated kinases

The goal of the present study is to test the hypothesis that LPA induces proliferation of astrocytes in hippocampus in vivo via phosphorylation of ERK 1/2. We first characterized the expression of GFAP, a special marker fiber protein of astrocytes, in brain slices after direct injection of LPA into hippocampus by immunohistochemistry, and found that LPA induced a remarkable proliferation of astrocytes. Then double-lablled immunofluorescence was used to detect GFAP and phosphorylation ERK 1/2 (p-ERK 1/2), LPA induced an immediate (10 min) and transient (<30 min) phosphorylation of ERK 1/2, and sequence sustained activation of ERK 1/2 was observed, which last for at least 3 weeks after injection of LPA. Reactions are inhibited by U0126, a specific pharmacological mitogen-activated protein kinase (MEK) inhibitor. Laser confocal scanning was used to study spatial relationship of p-ERK and astrocytes. Amazingly, the early (<7 days) phosphorylation of ERK 1/2 is not expressed in astrocytes but in area where neurons and/or in other cell type(s) occupied, expression of p-ERK 1/2 in astrocytes is not detected until 14 days after LPA injection and lasts for at least 3 weeks. Taken together, these data suggest that LPA play an important role in proliferation of astrocytes through phosphorylation of ERK 1/2 in hippocampus. It provides further proof for the functions of LPA in CNS injury, and may contribute to clinical therapy for relative diseases.     

Hydrogen bonded layer-by-layer assembly of poly(2-vinylpyridine) and poly(acrylic acid): Influence of molecular weight on the formation of microporous film by post-base treatment

This article describes the buildup of hydrogen bonded multilayer film of poly(2-vinylpyridine) (P2VP) and poly(acrylic acid) (PAA), and the influence of polymer molecular weight on the formation of microporous film by post-base treatment. The formation of a microporous film involved a two-step mechanism: the release of PAA from P2VP/PAA multilayer, and the reorganization of the remaining P2VP on the substrate. Fourier transform infrared spectroscopy (FT-IR) indicated that the release of PAA from hydrogen bonded multilayer was a rapid process, which was almost independent of the molecular weight of PAA. Furthermore, the molecular weight of P2VP had a great effect on micropore formation by immersing the P2VP/PAA multilayer in basic solution. The rate of micropore formation increased with increasing molecular weight. We anticipate that a comparative study on P2VP/PAA films containing high or low molecular weight polymer provides a way to control the surface morphology, and will be helpful and constructive for the forthcoming discussion about the formation of the microporous film.     

聚N—异丙基丙烯酰胺溶液的温度依赖关系

用自由基聚合法合成了聚Ｎ－异丙基丙烯酰胺（ＰＮＩＰＡＡＭ）样品，用乌氏粘度计考查了该聚合物的四氢呋喃（ＴＨＦ）溶液和水溶液的粘度与温度的依赖关系。发现ＰＮＩＰＡＡＭ－ＴＨＦ体系的特性粘数随温度升高而增大，ＰＮＩＰＡＡＭ－Ｈ２Ｏ体系的特性粘数－温度曲线表现出较为复杂的变化规律。并用实验确定的特性粘数对合成样品的分子量进行了表征Ｍｎ＝８．４４×１０＾５ｇ·ｍｏｌ＾－１。     

Determination of lutetium by fluorimetry, using BPMPHD and CTMAB

Lutetium(III) forms an association compound with a new synthetic reagent, 1,6-bi(1-phenyl-3-methyl-5-pyrazolone-4)hexandione (BPMPHD), and cetyltrimethylammonium bromide (CTMAB). The compound enhances the natural fluorescence of BPMPHD remarkably, upon which a new fluorescence method was developed for determining lutetium in rare earth (RE) samples. The determination range was 1.80 × 10^–7–8.8 × 10^–6 g/ml. The determination limit was 29 ng/ml. The composition of the ion associate was [Lu(BPMPHD)₂]–CTMAB⁺.     

小分子化合物在离子阱质谱中的分子离子反应研究

利用离子阱质谱的原理和特点，研究了小分子醇、醚、胺、醛、酮等有机化合 物（分子量小于200）在离子阱里的分子离子反应，总结了反应特点和规律，并把 它归类为自身化学电离（SCI）反应。以丁酮、丙烯醇为例，采用FTMS对反应产物 离子进行准确质量测定，验证了它们各自的分子离子反应结果。另外，把该类化合 物（30个）SCI反应的质谱图与NIST98库中的标准EI质谱图进行了比较，建立了 SCI质谱图库，提高了在离子阱质谱上对这类小分子化合物定性分析的准确率。     

Dependence of Elution Curve and Adsorption Isotherms of Insulin on composition of Mobile Phase of Frontal Analysis in Reversed Phase Liquid Chromatography

With frontal analysis(FA),the dependence of adsorption isotherms of insulin on the composition of mobile phase in reversed phase liquid chromatography (RPLC) has been investigated,This is also a good example to employ the stoichiometric displacement theory (SDT) for ivestigating solute adsorption in physical chemistry.Six kinds of mobile phase in RPLC were employed to study the effects on the elution curves and adsorption isotherms of insulin.the key points of this paper are:(1) the stability of insulin due to delay time after preparing,the organic solvent concentration,the kind and the concentration of ion-pairing agent in mobile phase were found to affect both elution curve and adsorption isotherm very seriously.(2)To obtain a valid and comparable result,the composition of the mobile phase employed in FA must be as same as possible to that in usual RPLC of either analytical scale or preparative purpose.(3)Langmuir Equation and the SDT were employed to imitate these obtained adsorption isotherms.The expression for solute adsorption from solution of the SDT was found to have a better elucidation to the insulin adsorption from mobile phase in RPLC.