Distance distributions and dynamics of a zinc finger peptide from fluorescence resonance energy transfer measurements

Time-resolved fluorescence resonance energy transfer (FRET) measurements were used to measure distance distributions and intramolecular dynamics (site-to-site diffusion) of a 28-residue single-domain zinc finger peptide in the absence and presence of zinc ion. Energy transfer was measured between TRP₁₄ and a N-terminal DNS group. As expected, the TRP-to-DNS distance distribution for zinc-bound peptide is shorter and narrower (R _av=11.2 Å,hw=2.8 Å) than the metal-free peptide (R _av=20.1 Å,hw=14.5 Å). The degree of mutual donor-to-acceptor diffusion (D) was also determined for these distributions. For zinc-bound peptide there is no detectible diffusion (D0.2 Ų/ns), whereas for metal-free peptide a considerable amount of motion is occurring between the donor and the acceptor (D=12 Ų/ns). These results indicate that the zinc-bound peptide folds into a unique, well-defined conformation, whereas the metal-free conformation is flexible and rapidly changing. The absence of detectible mutual site-to-site diffusion between the donor and the acceptor in the metal-bound zinc finger peptide indicates that intramolecular motion is essentially frozen out, on the FRET time scale, as a consequence of zinc coordination.Dedicated to the memory of Barbara D. Wells.     

天然有机化合物NOESY谱的全弛豫矩阵分析

本文用定量处理2D NOE实验数据最精确的方法-全弛豫矩阵分析法定量分析了天然有机化合物显脉香茶菜素的相敏NOESY谱,计算出显脉香茶菜素分子中各质子间的交叉弛豫速率,根据1/r_tf⁶∝σ_tf计算出相应的质子间距离,结果表明:用全弛豫矩阵分析法计算出的质子间距与分子力学计算以及用单晶X-射线衍射法测得的质子间距离基本一致,为用核磁共振方法确定分子在溶液中的三维空间结构提供了可靠的结构参数。     

透镜初级球差的横向剪切干涉条纹研究

利用玻璃平行平板构成简单的横向剪切干涉仪可以观察到单薄透镜形成的准直光束的剪切干涉条纹,由干涉条纹分布求出对应的几何像差和离焦量.用焦距为190 mm的单薄透镜做实验,实验结果与计算机模拟结果符合,说明可以从剪切干涉条纹的分布求出透镜的轴向调整误差和初级球差.     

基于OSLO的无限远像距消色差显微物镜的设计

共焦激光扫描显微镜对物镜有高分辨率、易于实现纵向扫描的要求,为此设计了高分辨率6片式无限远像距显微物镜.它的前置物镜与辅助物镜之间具有一段平行光路,因此容易实现轴向扫描.根据使用要求总体计算该物镜的外形尺寸,以像差理论为基础选择6片式的消色差物镜初始结构,用专业光学设计软件OSLO对物镜和辅助物镜进行设计和像质分析.设计结果显示,独特的6片式结构的20×物镜分辨率达到了800 lp/mm,符合共焦显微镜的使用要求.     

基于HI-13串列加速器在束γ终端原子核激发态寿命测量的核结构研究

利用在HI-13串列加速器的在束γ终端上建立的多普勒线移衰减法、反冲距离多普勒线移法和快时间延迟符合法等3种寿命测量方法,开展了原子核手征对称性、磁转动等核结构热点课题的研究工作。实验结果表明,130Cs的伙伴带具有很好的手征特性,而106Ag的候选带并非手征双带。根据测量的能级寿命提取的B（M1）值表明,106Ag的正宇称晕带和107Ag的正、负宇称晕带都具有典型的磁转动特性。此外,寿命测量数据还为解释和理解不同的同位素链中展现出的形状演化、形状共存、形状相变及临界点对称性等物理现象提供了有价值的实验证据。 The significance and principle of lifetime measurements of excited states in nuclei are briefly described. By using the recoil-distance Doppler-shift method, the Doppler-shift attenuation method, and the fast-timing technique established at the in-beam γ terminal of HI-13 tandem accelerator, nuclear structure studies on topical subjects including chiral symmetries and magnetic rotation have been performed. Our experimental results indicate that 130Cs shows better chiral characteristics, however, the two candidate bands of 106Ag could not be a pair of chiral doublet bands. For magnetic rotation, the deduced B(M1) values deduced from the measured level lifetimes clearly demonstrate that the yrast positive-parity band in 106Ag and both the yrast positiveand negative-parity bands in 107Ag are magnetic rotation bands. In addition, our lifetime measurement data also provide valuable experimental evidences for the interpretation and understanding of the shape evolution, the shape coexistence, and the critical-point symmetries of shape phase transition in the different isotope chains.     

对物体沿散斑片纵向移动二次曝光全息干涉条纹的诠释

从光学几何关系出发,推导了在平行光照明、物体沿散斑片纵向方向微小移动情况下的一种全息干涉条纹的解释方法.该方法可以根据任一瞬间的条纹间距测量物体的位移,并适用于实时全息监测的定量分析,对全息干涉计量术提供有效的条纹解释方法和物体位移的计算方法.     

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通过数值模拟及实验研究了润湿性及磁场对液态金属膜流流动状态的影响.首先,通过数值模拟研究了润湿性对膜流流动状态的影响.结果表明,当润湿性不好时,液态金属膜流容易发展为溪状流而不能完全覆盖底壁,入口膜厚较薄时更易发展为溪状流;在入口膜厚及其它情况相同时,密度越小越易发展为溪状流.其次,研究了磁场对膜流流动状态的影响.结果表明,槽道与流体润湿性不好时,有磁场情况下液态金属膜流覆盖底壁的区域较无磁场时增加,强磁场对膜流的湍流有抑制作用.最后,液态金属膜流实验结果表明,润湿性不好时,镓铟锡合金膜流容易收缩发展为溪状流,这与数值模拟的结果是一致的.上述研究结果对磁约束聚变堆液态第一壁的设计具有指导意义.     

基于跳距修正L-M优化的WSN定位算法

针对DV-Hop定位算法在节点随机分布的网络拓扑环境下存在误差较大的问题,文中通过分析平均跳距估计、未知节点坐标估计区域求解对定位精度的影响,提出了一种基于跳距修正L-M优化的WSN定位算法CLDV-Hop(Correct L-M DV-Hop)。仿真结果表明,在不增加额外开销且仿真环境相同的条件下,CLDV-Hop算法比现有改进的算法具有更高的定位精度,与DV-Hop算法相比精度提高了约33%——41%。     

有距离辨识度的传声器阵列波束优化算法研究*

小尺度线性传声器阵列在便携式通信设备中具有潜在应用前景,其波束优化算法大多关注于抑制来自与声源不同方向的噪声,而不能有效地抑制与声源同方向的噪声。本文通过对几种典型波束优化策略进行改进,增加线性约束条件,使得阵列兼具方向和距离辨识度。论文对比了几种优化方法的波束性能,同时讨论了约束条件对波束旁瓣的影响,并提出了鲁棒性的优化方案。仿真结果显示,合理的优化策略和线性约束条件可以使阵列波束获得很好的聚焦性能。