Cloning, Expression and Purification of Wheat Acetyl-CoA Carboxylases CT Domain in E.coil

The entire gene of carboxyltransferase(CT) domain of acetyl-CoA carboxylase(ACCase) from Chinese Spring wheat(CSW) plastid was cloned firstly,and the 2.3 kb gene was inserted into PET28a+ vector and expressed in E.coil in a soluble state.The (His)6 fusion protein was identified by SDS-PAGE and Western blot.The recombinant protein was purified by affinity chromatography,and the calculated molecular mass(Mr) was 88000.The results of the sequence analysis indicate that the cloned gene(GeneBank accession No.EU124675) was a supplement and revision of the reported ACCase CT partial cDNA from Chinese Spring wheat plastid.The recombinant protein will be significant for us to investigate the recognizing mechanism between ACCase and herbicides,and further to screen new herbicides.     

Domain Adaptation with Data Uncertainty Measure Based on Evidence Theory

Domain adaptation aims to learn a classifier for a target domain task by using related labeled data from the source domain. Because source domain data and target domain task may be mismatched, there is an uncertainty of source domain data with respect to the target domain task. Ignoring the uncertainty may lead to models with unreliable and suboptimal classification results for the target domain task. However, most previous works focus on reducing the gap in data distribution between the source and target domains. They do not consider the uncertainty of source domain data about the target domain task and cannot apply the uncertainty to learn an adaptive classifier. Aimed at this problem, we revisit the domain adaptation from source domain data uncertainty based on evidence theory and thereby devise an adaptive classifier with the uncertainty measure. Based on evidence theory, we first design an evidence net to estimate the uncertainty of source domain data about the target domain task. Second, we design a general loss function with the uncertainty measure for the adaptive classifier and extend the loss function to support vector machine. Finally, numerical experiments on simulation datasets and real-world applications are given to comprehensively demonstrate the effectiveness of the adaptive classifier with the uncertainty measure.     

Cloning, Expression and Purification of Wheat Acetyl-CoA Carboxylases CT Domain in E. coil

The entire gene of carboxyltransferase（CT） domain of acetyl-CoA carboxylase（ACCase） from Chinese Spring wheat（CSW） plastid was cloned firstly, and the 2.3 kb gene was inserted into PET28a^＋ vector and expressed in E. coil in a soluble state. The （His）6 fusion protein was identified by SDS-PAGE and Western blot. The recombinant protein was purified by affinity chromatography, and the calculated molecular mass（Mr） was 88000. The results of the sequence analysis indicate that the cloned gene（GeneBank accession No. EU124675） was a supplement and revision of the reported ACCase CT partial cDNA from Chinese Spring wheat plastid. The recombinant protein will be significant for us to investigate the recognizing mechanism between ACCase and herbicides, and further to screen new herbicides.     

A Ferroelectric Frequency-Doubling Material-Potassium Lithium Niobate

The potassium lithium niobate crystals have been grown up. The shapes of solid-melt interfaces which maintaining the steady growth of the potassium lithium niobate crystals have been described. The optical transmission spectrum of the crystal has been surveyed. The perfected crystals showed good Second Harmonic Generation properties.     

Planar Hall effect in biosensor with a tilted angle of the cross-junction

A typical planar Hall effect (PHE) sensor junction consists of two Hall bars that the bars appear normal to each other and the junction can have the required four terminals for current and voltage measurements. We are now introducing a tilted angle of the cross-junction and studying the role of the PHE therein. The results show that although there is a tilted angle of the cross-junction, the PHE voltage is remained constant. The result is interpreted by assuming the sensor material with the behavior of a basic single domain structure under the external magnetic field reversals. The calculations of the model are found to be in good concurrence with the experimental results.     

Efficient thermal field computation in phase-field models

We solve the phase-field equations in two dimensions to simulate crystal growth in the low undercooling regime. The novelty is the use of a fast solver for the free space heat equation to compute the thermal field. This solver is based on the efficient direct evaluation of the integral representation of the solution to the constant coefficient, free space heat equation with a smooth source term. The computational cost and memory requirements of the new solver are reasonable and no artificial boundary conditions are needed. This allows one to solve for the thermal field in a computational domain whose size depends only on the size of the growing crystal and not on the extent of the thermal field, which can result in significant computational savings in the low undercooling regime.     

Kerr microscopy study of magnetization reversal in uniaxial Co-films

We have studied the magnetization reversal of uniaxial Co(1 0 1 0) films as a function of the applied field orientation by means of magneto-optical Kerr effect microscopy. Hereby, we find that while stable intermediate domain states exist for most field directions, their occurrence is suppressed for field orientations along the easy axis of magnetization. To facilitate this study, we have developed a data extraction methodology that allows for the quantitative analysis and compact display of entire magnetization distribution field-sequences in a single picture. It furthermore allows for the automated data analysis to unambiguously distinguish magnetization rotation processes from field-induced domain formation.     

Multifractal formalism for self-similar measures with weak separation condition

For any self-similar measure μ on satisfying the weak separation condition, we show that there exists an open ball U₀ with μ(U₀)>0 such that the distribution of μ, restricted on U₀, is controlled by the products of a family of non-negative matrices, and hence μ|_U0 satisfies a kind of quasi-product property. Furthermore, the multifractal formalism for μ|_U0 is valid on the whole range of dimension spectrum, regardless of whether there are phase transitions. Moreover the dimension spectra of μ and μ|_U0 coincide for q0. This result unifies and improves many of the recent works on the multifractal structure of self-similar measures with overlaps.     

Hermite and Smith normal form algorithms over Dedekind domains

We show how the usual algorithms valid over Euclidean domains, such as the Hermite Normal Form, the modular Hermite Normal Form and the Smith Normal Form can be extended to Dedekind rings. In a sequel to this paper, we will explain the use of these algorithms for computing in relative extensions of number fields.