新型分子高光谱成像系统性能分析及数据预处理

将分子成像技术和高光谱技术相结合,研制了基于AOTF(Acousto-optic Tunable Filters)的分子高光谱成像系统。系统由显微镜、分光仪、CCD镜头、图像数据采集卡和计算机等几部分组成。在综合考虑各功能部件的性能及相互的制约关系的基础上,分析了系统的性能指标,系统的光谱范围从550～1 000 nm,可采集200个波段,空间分辨率可达0.061 5 μm,光谱分辨率可达2 nm,当CCD工作在积分模式下采集速度可达到2.612 5 s·B-1,当CCD工作在非积分模式下可达到约0.11 μs·B-1。由于受系统光源和光路中透镜及传感器性能的影响,采集到的图像数据需要进行预处理,文中提出一种空间维和光谱维联合校正的灰度校正系数算法,并给出算法的具体实现。以白血病的血液作样本,通过对比校正前后的单波段图像、伪彩色图像和光谱曲线,说明校正算法的有效性,为后续的光谱图像数据分析提供了有效的数据。     

A Projected Algebraic Multigrid Method for Linear Complementarity Problems

We present an algebraic version of an iterative multigrid method for obstacle problems, called projected algebraic multigrid (PAMG) here. We show that classical algebraic multigrid algorithms can easily be extended to deal with this kind of problem. This paves the way for efficient multigrid solution of obstacle problems with partial differential equations arising, for example, in financial engineering.     

C18反相色谱固定相的制备及性能评价——介绍一个仪器分析拓展性实验

介绍了一个本科生仪器分析拓展性实验——C_(18)反相色谱固定相的制备及性能评价。实验内容包含色谱固定相的合成、表征、装填及其性能评价,实验过程涵盖了有机合成、物理化学和仪器分析知识。通过本实验的实践,拓展了经典仪器分析实验内容,有利于培养学生综合运用知识的能力和科研探究能力。     

A covalently cross‐linked microporous polymer based micro‐solid phase extraction for online analysis of trace pesticide residues in citrus fruits

Covalently cross‐linked microporous polymers are a new class of highly cross‐linked porous network materials with large surface area and potential superiority in sample pretreatment. In this work, a covalently cross‐linked microporous polymer was well designed and synthesized by condensation of acylhydrazines in terephthalic dihydrazide with aldehyde groups in 1,3,5‐benzenetricarboxaldehyde. The adsorption mechanism was explored and discussed based on π‐π stacking interaction and steric effect. Then, a covalently cross‐linked microporous polymer was employed as the adsorbent of online micro‐solid‐phase extraction coupled with high‐performance liquid chromatography for the enrichment and analysis of trace pesticide residues in citrus fruits. The method was successfully applied to the online analysis of sugar orange and Huangdigan samples with the detection limits of 0.10–0.30 μg/kg. It was satisfactory that chlorpifos and triazophos in real sugar orange and Huangdigan samples could be actually found and quantified at concentrations of 0.20 and 0.51 mg/kg, respectively. The recoveries of sugar orange and Huangdigan samples were in the range of 70.0–103 and 74.0–119% with relative standard deviations of 0.4–9.7 and 0.5–9.2% (n = 3), respectively. The proposed method was accurate, reliable, and convenient for the online simultaneous analysis of trace pesticide residues in citrus fruits.     

Comparative numerical study of single and two-phase models of nanofluid heat transfer in wavy channel

The main purpose of this study is to survey numerically comparison of two- phase and single phase of heat transfer and flow field of copper-water nanofluid in a wavy channel. The computational fluid dynamics （CFD） prediction is used for heat transfer and flow prediction of the single phase and three different two-phase models （mixture, volume of fluid （VOF）, and Eulerian）. The heat transfer coefficient, temperature, and velocity distributions are investigated. The results show that the differences between the temperature fie].d in the single phase and two-phase models are greater than those in the hydrodynamic tleld. Also, it is found that the heat transfer coefficient predicted by the single phase model is enhanced by increasing the volume fraction of nanoparticles for all Reynolds numbers; while for the two-phase models, when the Reynolds number is low, increasing the volume fraction of nanoparticles will enhance the heat transfer coefficient in the front and the middle of the wavy channel, but gradually decrease along the wavy channel.     

抗黄曲霉毒素B1多价纳米抗体融合蛋白的构建及活性分析

纳米抗体来源于天然缺失轻链的重链抗体可变区,是已知最小抗原结合单元.该研究构建了抗黄曲霉毒素B1(AFB1)纳米抗体的单价及多价串联体,分别与绿色荧光蛋白(GFP)编码片段融合并克隆至原核表达载体pET30.以大肠杆菌BL21(DE3)作为表达宿主,通过异丙基-β-D硫代吡喃半乳糖苷诱导,亲和层析技术分别纯化单、双及三...     

Enhanced Multi-phosphopeptide Enrichment and Nano LC-ESI-qTOF-MS Detection Strategy Using Click OEG-CD Matrix

Detection and determination of multi-phosphopeptides from protein digestion products are difficult due to the low abundance and ion-suppression effect arised from the existence of their high-abundance counterparts. Click OEG-CD[olio(ethylene glycol)(OEG) linked β-cyclodextrin(CD)] matrix has shown excellent performance in phosphopeptide enrichment. However, few multi-phosphopeptides were detected previously. In this investigation, an improved method aiming at enhancing the enrichment selectivity and mass spectrometry(MS) detection of multi-phosphopeptide was developed via optimizing the sample loading amount on per mg of matrix. Mass spectra were obtained on a Nano liquid chromatography-electrospray ionization-quadrupole time of flight-mass spectrometry (LC-ESI-qTOF-MS). The enrichment selectivity of double-phosphopeptide could be enhanced with the increase of loading amount on per mg of matrix when taking the mixture of mono-, double-, and non-phosphopeptides as probe. Furthermore, the multi-phosphopeptide enrichment selectivity was enhanced under the condition of optimized loading amount when taking the product of typtic digestion α-casein as test sample. When the loading amount was 573 pmol/mg matrix, up to 20 α-casein phosphopeptide signals(including 15 multi-phosphopeptides) were detected. The result is much better than that of our previous report. The reduction of ion-suppression effect arised from the existence of high-abundance non-or mono-phosphopeptides and the stronger interactions between multi-phosphopepides and the matrix were contributed to the result. The study could be helpful to the better utilization of Click OEG-CD matrix in the enrichment of multi-phosphopeptide from complex biosamples in the subsequent investigation.     

重置期权的随机性研究

利用等价测度和鞅的方法,以股票价格为选择重设点依据的情况下推导了随机时间重置期权中的欧式看涨期权的定价公式.     

太赫兹时域光谱与频域光谱研究综述

近年来太赫兹技术因其重要的理论研究价值和广泛的应用前景引起了科学界的普遍关注。太赫兹光谱技术作为太赫兹科学发展的主要方向之一,可分为频域光谱与时域光谱两种。它的出现解决了太赫兹波段下无法产生宽带辐射源的难题,使得光谱学上存在的太赫兹断层得以填补。随着这项技术的发展,对太赫兹波段下物质特性的研究也逐步拓展到生物医学、材料、通信、安检为代表的各个领域。从产生原理、性能特点、应用领域等方面对两种光谱进行比较,进而阐述了两种太赫兹光谱的优缺点以及其应用优势。     

凹筒型换能器性能参数与开缝数之间关系的研究

凹筒形换能器具有频率低、功率大、尺寸小、重量轻等诸多优点,且其独特的外型结构适合于组排基阵,有较好的应用前景。为易于产生径向弯曲振动,通常沿轴向等间距开缝,以降低径向刚度。开缝数太少,不利于达到预期目的;开缝数太多,又可能引起声源级的降低。本文计算了凹筒型换能器开缝数与换能器声性能参数之间的关系。实验测试与理论计算比较符合。