Separation of intact proteins on porous layer open tubular (PLOT) columns

Porous layer open tubular (PLOT) polystyrene divinylbenzene columns have been used for separating intact proteins with gradient elution. The 10 μm I.D. × 3 m columns were easily coupled to standard liquid chromatography–mass spectrometry (LC–MS) instrumentation with commercially available fittings. Standard proteins separated on PLOT columns appeared as narrow and symmetrical peaks with good resolution. Average peak width increased linearly with gradient time (t_G) from 0.14 to 0.33 min (t_G 20 and 120 min, respectively) using a 3 m column. With shorter columns, peak widths were larger and increased more steeply with gradient time. Theoretical peak capacity (n_c) increased with column length (tested up to 3 m). The n_c increased with t_G until a plateau was reached. The highest peak capacity achieved (n_c = 185) was obtained with a 3 m column, where a plateau was reached with t_G 90 min. The within- and between column retention time repeatabilities were below 0.6% and below 2.5% (relative standard deviation, RSD), respectively. The carry-over following injection of 0.5 ng per protein was less than 1.1%. The retention time dependence on column temperature was investigated in the range 20–50 °C. Proteins in a skimmed milk sample were separated using the method.     

Carboxymethylchitosan covalently modified capillary column for open tubular capillary electrochromatography of basic proteins and opium alkaloids

A novel open tubular (OT) column covalently modified with hydrophilic polysaccharide, carboxymethylchitosan (CMC) as stationary phase has been developed, and employed for the separations of basic proteins and opium alkaloids by capillary electrochromatography (CEC). With the procedures including the silanization of 3-aminopropyltrimethoxysilane (APTS) and the combination of glutaraldehyde with amino-silylated silica surface and CMC, CMC was covalently bonded on the capillary inner wall and exhibited a remarkable tolerance and chemical stability against 0.1 mol/L HCl, 0.1 mol/L NaOH or some organic solvents. By varying the pH values of running buffer, a cathodic or anodic EOF could be gained in CMC modified column. With anodic EOF mode (pH<4.3), favorable separations of basic proteins (trypsin, ribonuclease A, lysozyme and cytochrome C) were successfully achieved with high column efficiencies ranging from 97,000 to 182,000 plates/m, and the undesired adsorptions of basic proteins on the inter-wall of capillary could be avoided. Good repeatability was gained with RSD of the migration time less than 1.3% for run-to-run (n=5) and less than 3.2% for day-to-day (n=3), RSD of peak area was less than 5.6% for run-to-run (n=5) and less than 8.8% for day-to-day (n=3). With cathodic EOF mode (pH>4.3), four opium alkaloids were also baseline separated in phosphate buffer (50 mmol/L, pH 6.0) with column efficiencies ranging from 92,000 to 132,000 plates/m. CMC-bonded OT capillary column might be used as an alternative medium for the further analysis of basic proteins and alkaline analytes.     

Improvements on open and traction boundary conditions for Navier–Stokes time-splitting methods

We present in this paper a numerical scheme for incompressible Navier–Stokes equations with open and traction boundary conditions, in the framework of pressure-correction methods. A new way to enforce this type of boundary condition is proposed and provides higher pressure and velocity convergence rates in space and time than found in the present state of the art. We illustrate this result by computing some numerical and physical tests. In particular, we establish reference solutions of a laminar flow in a geometry where a bifurcation takes place and of the unsteady flow around a square cylinder.     

Holomorphic mappings preserving Minkowski functionals

We show that the equality _m1(f(x))=_m2(g(x))$m_1\left(f\left(x\right)\right)=m_2\left(g\left(x\right)\right)$ for x$x$ in a neighborhood of a point a$a$ remains valid for all x$x$ provided that f$f$ and g$g$ are open holomorphic maps, f(a)=g(a)=0$f\left(a\right)=g\left(a\right)=0$ and _m1,_m2$m_1,m_2$ are Minkowski functionals of bounded balanced domains. Moreover, a polynomial relation between f$f$ and g$g$ is obtained.     

Stochastic quantum molecular dynamics for finite and extended systems

We present a detailed account of the technical aspects of stochastic quantum molecular dynamics, an approach introduced recently by the authors [H. Appel, M. Di Ventra, Phys. Rev. B 80 (2009) 212303] to describe coupled electron-ion dynamics in open quantum systems. As example applications of the method we consider both finite systems with and without ionic motion, as well as describe its applicability to extended systems in the limit of classical ions. The latter formulation allows the study of important phenomena such as decoherence and energy relaxation in bulk systems and surfaces in the presence of time-dependent fields.     

Fast separations with open tubular ion exchange capillary columns

Fused silica columns of 4.6 μm i.d. were coated with 3-sulfopropylsilane as a cation exchanger and 3-(2-aminoethyl-amino)-propylsilane as an anion exchanger. Fast separations of cations (< 25 seconds) and of anions (< 35 seconds) were obtained, using a potentiometric microelectrode as a detector. In proceeding towards smaller i.d.s, a mixture of alkali metal cations could be separated successfully in an uncoated fused silica capillary of 2.3 μm i.d. as a result of retention by the surface silanol groups at pH 9.4.     

图书采购流程中资金分配模型研究

本应用现代经济理论的择优分配原理，在图书产出效益函数的性质探讨的基础上，将效益函数的约束条件转化为对资金变量的约束条件，从而简化了资金分配方案的求解。提出了资金在各类别图书中的分配模型，指导图书馆图书采购工作。     

The displacement discontinuity method in the analysis of open cracks

The application of the displacement discontinuity numerical technique to the solution of some problems of fracture mechanics is demonstrated in the hypothesis of homogeneous and elastic material. The fracture is supposed to be free from traction and is represented by a set of constant displacement discontinuity elements, except for two parabolic elements, located at each crack tip, in order to simulate the singularity of the solution near the crack tips. On the basis of the stress and displacement field determined by the displacement discontinuity method, the stress intensity factors for mode I and II are computed according to the method of the displacements. Three examples are provided to verify the validity of the formulation.

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Sommario Lo scopo del presente lavore è di illustrare l'applicazione del metodo numerico della Displacement Discontinuity alla soluzione di alcuni problemi di meccanica della frattura, nell'ipotesi di materiale omogeneo ed elastico. La frattura è supposta aperta ed è rappresentata da una linea di elementi a discontinuità di spostamento costante, con l'eccezione di due speciali elementi parabolici, ubicati agli apici, al fine di simulare la singolarita' del campo tensionale. Sulla base del campo degli sforzi e degli spostamenti cosi determinati, vengono ricavati i fattori di concentrazione degli sforzi in modo I e II mediante il metodo degli spostamenti. Vengono inoltre riportati tre esempi di calcolo, effettuati al fine di verificare la validità del procedimento proposto.

     

Open channel turbulent flow over hemispherical ribs

This paper reports an experimental investigation of open channel turbulent flow over hemispherical ribs. A row of ribs consists of hemispheres closely placed to one another in the spanwise direction and cover the entire span of the channel. The pitch-to-height ratio is varied to achieve the so-called d-type, intermediate and k-type roughness. The Reynolds numbers based on water depth, h, and momentum thickness, θ, of the approach flow are respectively, Re_h = 28,100 and Re_θ = 1800. A particle image velocimetry is used to obtain detailed velocity measurements in and above the cavity. Streamlines, mean velocity and time-averaged turbulent statistics are used to study the effects of pitch-to-height ratio on the flow characteristics and also to document similarities and differences between the present work and prior studies over two-dimensional transverse rods. It was observed that interaction between the outer flow and the shear layers generated by ribs is strongest for k-type and least for d-type ribs. The results also show that hemispherical ribs are less effective in augmenting flow resistance compared to two-dimensional transverse ribs. The levels of the Reynolds stresses and budget terms increase with increasing pitch-to-height ratio inside the roughness sublayer.