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101.
102.
I. Kanakis C. Stavropoulou F. N. Lamari Y. Heliopoulos H. Iordanidou H. Piperidou D. Monos N. K. Karamanos 《Chromatographia》2003,58(7-8):513-516
Summary Multiple sclerosis (MS) is an autoimmune disease characterized by the production of specific types of immunoglobulins into the central nervous system. These immunoglobulins appear as oligoclonal bands (OCBs) in agarose isoelectric focusing (IEF) of cerebrospinal fluid (CSF). Among the cases with clinically definite MS, up to 95% have oligoclonal IgG bands in their CSF. In this report, we describe a micellar electrokinetic capillary chromatography (MEKC) method for the separation of CSF and serum proteins. MEKC was performed using 25 mM borate buffer, pH 10, containing 25 mM SDS at 20 kV and normal polarity. High values of repeatability in migration times and of reproducibility in peak areas were obtained (R.S.D. values were less than 2%). Calibration graphs were linear up to 2000 mg L–1. LOQ was 6.5 mg L–1 and LOD determined as a signal to noise ration of 3:1 was 4.5 mg L–1. Analysis of CSF and serum samples from patients with clinical definite MS and healthy individuals demonstrated the presence of two peaks migrating as -globulins in the CSF samples of patients. These peaks were absent from controls and the serum of the same patients. Correlation of the data obtained from IEF and MEKC analysis for 25 patients showed that the diagnostic sensitivity and specificity of MEKC were ca 89% and 92% respectively. The obtained results indicate that this MEKC method may be helpful for the diagnosis of multiple sclerosis. Capillary electrophoresis compared to flat bed IEF provides reproducible results, requires shorter analysis time, and allows direct quantitative determination.Presented at: International Symposium on Separation and Characterization of Natural and Synthetic Macromolecules, Amsterdam, TheNetherlands, February 5–7, 2003 相似文献
103.
Summary INDO molecular orbital calculations are reported for 35 selected angiotensin-converting enzyme inhibitors. QSARs are developed between pI50 data and molecular electronic indices. The QSARs obtained reflect the importance of both charge-charge interactions between inhibitor and receptor and of specific interactions between groups on the inhibitor with points around the molecule which are postulated to correspond to binding sites at the receptor. 相似文献
104.
Eugene P. Gross 《Journal of statistical physics》1981,25(4):585-604
We present a microscopic theory of the problem of finding the properties of a particle interacting with potentials located at random sites. The sites are governed by a general probability distribution. The starting point is the multiple scattering equations for the amplitude k
1|T
|k
2 in terms of the individual scattering amplitudes k
1|T
|k
2. We work with quantitiesA
defined by k
1|T
|k
2=k
1|T
|k
2exp[i(k
1–k
2)R
]. The theory is based on a splitting of the fundamental equation forA into equations for the mean A and the fluctuationsAA
. Neglect of the fluctuations yields the quasicrystalline approximation. We rearrange the equation forAA
to isolate the collective part of the fluctuations. We then make the simplest microscopic truncation which is thatAA
is a restricted two-body additive function of the site positions. With the contribution of the collective fluctuations, this yields results forA that are accurate to ordert
4.Work supported in part by the National Science Foundation under Contract No. NSF DMRWork supported in part by the National Science Foundation under Contract No. NSF DMR 相似文献
105.
Pekka J. Korhonen Kari Silvennoinen Jyrki WalleniusAnssi Öörni 《European Journal of Operational Research》2012
We investigate in a simple bi-criteria experimental study, whether subjects are consistent with a linear value function while making binary choices. Many inconsistencies appeared in our experiment. However, the impact of inconsistencies on the linearity vs. non-linearity of the value function was minor. Moreover, a linear value function seems to predict choices for bi-criteria problems quite well. This ability to predict is independent of whether the value function is diagnosed linear or not. Inconsistencies in responses did not necessarily change the original diagnosis of the form of the value function. Our findings have implications for the design and development of decision support tools for Multiple Criteria Decision Making problems. 相似文献
106.
Ignacy Kaliszewski Janusz MiroforidisDmitry Podkopaev 《European Journal of Operational Research》2012,216(1):188-199
We present an approach to interactive Multiple Criteria Decision Making based on preference driven Evolutionary Multiobjective Optimization with controllable accuracy.The approach relies on formulae for lower and upper bounds on coordinates of the outcome of an arbitrary efficient variant corresponding to preference information expressed by the Decision Maker. In contrast to earlier works on that subject, here lower and upper bounds can be calculated and their accuracy controlled entirely within evolutionary computation framework. This is made possible by exploration of not only the region of feasible variants - a standard within evolutionary optimization, but also the region of infeasible variants, the latter to our best knowledge being a novel approach within Evolutionary Multiobjective Optimization.To illustrate how this concept can be applied to interactive Multiple Criteria Decision Making, two algorithms employing evolutionary computations are proposed and their usefulness demonstrated by a numerical example. 相似文献
107.
A dual hesitant fuzzy set (DHFS) consists of two parts, that is, the membership hesitancy function and the nonmembership hesitancy function, supporting a more exemplary and flexible access to assign values for each element in the domain, and can handle two kinds of hesitancy in this situation. It can be considered as a powerful tool to express uncertain information in the process of group decision making. Therefore, we propose a correlation coefficient between DHFSs as a new extension of existing correlation coefficients for hesitant fuzzy sets and intuitionistic fuzzy sets and apply it to multiple attribute decision making under dual hesitant fuzzy environments. Through the weighted correlation coefficient between each alternative and the ideal alternative, the ranking order of all alternatives can be determined and the best alternative can be easily identified as well. Finally, a practical example of investment alternatives is given to demonstrate the practicality and effectiveness of the developed approach. 相似文献
108.
Behnam Vahdani S. Meysam Mousavi R. Tavakkoli-Moghaddam H. Hashemi 《Applied Mathematical Modelling》2013
The purpose of this paper is to design a new extension of the ELECTRE, known as the elimination and choice translating reality method, for multi-criteria group decision-making problems based on intuitionistic fuzzy sets. This method is widely utilized when a set of alternatives should be identified and evaluated with respect to a set of conflicting criteria by reflecting decision makers’ (DMs’) preferences. However, handling the exact data and numerical measure is difficult to be precisely focused because the DMs’ judgments are often vague in real-life decision problems and applications. A more realistic and practical approach can be to use linguistic variables expressed in intuitionistic fuzzy numbers instead of numerical data to model DMs’ judgments and to describe the inputs in the ELECTRE method. The proposed intuitionsitic fuzzy ELECTRE utilizes the truth-membership function and non-truth-membership function to indicate the degrees of satisfiability and non-satisfiability of each alternative with respect to each criterion and the relative importance of each criterion, respectively. Then, a new discordance intuitionistic index is introduced, which is extended from the concept of the fuzzy distance measure. Outranking relations are defined by pairwise comparisons and a decision graph is depicted to determine which alternative is preferable, incomparable or indifferent in the intuitionistic fuzzy environment. Finally, a comprehensive sensitivity analysis is employed to further study regarding the impact of threshold values on the final evaluation, and a comparative analysis is demonstrated with an application example in flexible manufacturing systems between the proposed ELECTRE method and the existing intuitionistic fuzzy technique for order preference by similarity to ideal solution (IF-TOPSIS) method. 相似文献
109.
In this paper we construct a first solution of the stochastic realization problem in a nonlinear setting. The great bulk of previous work on stochastic realization has been in the linear Gaussian setting. Such Markovian representations are used e.g., to apply certain filtering and stochastic control techniques. Our methods consist of an amalgamation of methods introduced by Nelson with the Lax-Phillips type geometric approach to linear Gaussian stochastic realization which has been developed by Lindquist and Picci and by Ruckebusch. The result of this that we are able to realize any purely nondeterministic process satisfying suitable continuity conditions as an instantaneous function of a Markov process. 相似文献
110.
This paper describes changes in students’ geometrical knowledge and their creativity associated with implementation of Multiple Solution Tasks (MSTs) in school geometry courses. Three hundred and three students from 14 geometry classes participated in the study, of whom 229 students from 11 classes learned in an experimental environment that employed MSTs while the rest learned without any special intervention in the course of one school year. This longitudinal study compares the development of knowledge and creativity between the experimental and control groups as reflected in students’ written tests. Geometry knowledge was measured by the correctness and connectedness of the solutions presented. The criteria for creativity were: fluency, flexibility, and originality. The findings show that students’ connectedness as well as their fluency and flexibility benefited from implementation of MSTs. The study supports the idea that originality is a more internal characteristic than fluency and flexibility, and therefore more related with creativity and less dynamic. Nevertheless, the MSTs approach provides greater opportunity for potentially creative students to present their creative products than conventional learning environment. Cluster analysis of the experimental group identified three clusters that correspond to three levels of student performance, according to the five measured criteria in pre- and post-tests, and showed that, with the exception of originality, performance in all three clusters generally improved on the various criteria. 相似文献