基质刚性影响声孔效应介导的单细胞基因转染

实验研究了基质刚性对单细胞质粒DNA转染效果的影响。实验采用高声压短脉冲(0.45MPa,10μs)条件的超声对培养在不同硬度凝胶基质(软的凝胶基质：0.2kPa,硬的凝胶基质：40kPa)上的力学敏感细胞NIH 3T3进行质粒DNA转染实验。实验结果表明,培养在硬的凝胶基质上的细胞,质粒DNA转染效率明显高于培养在软的凝胶基质上的细胞。进一步对质粒DNA进行荧光示踪可知培养在不同刚性基质上的细胞导入质粒DNA的方式不同。当细胞被培养在硬的凝胶基质上时,通过声致穿孔产生的小孔进入细胞内的质粒DNA更多,而培养在软的凝胶基质上的细胞,更多的质粒DNA可以通过非声致穿孔作用,例如内吞方式导入细胞。 细胞骨架蛋白分布规律表明,硬的凝胶基质上培养的细胞内有更多的F肌动蛋白微丝,可以更好地支撑起细胞的铺展形态,相对不容易发生内吞作用。而软的凝胶基质上培养的细胞内F肌动蛋白则更多以球形状态存在,细胞形貌骗向圆形,此时更容易发生胞吞作用。     

基于三维接触有限元分析的管片等效抗弯刚度和错台研究

针对盾构隧道管片接头等效抗弯刚度预测研究中,对梁-弹簧与三维模型整体等效性考虑的不充分,以三维接触有限元计算结果作为测量信息,借助基于挠度等效的反问题求解,提出了一种确定管片接头等效抗弯刚度的新方法;并利用不同轴力-偏心距组合下的反演结果,建立了基于Kriging代理模型的轴力-弯矩-等效抗弯刚度的非线性关系,提出了由此关联的管片结构非线性问题的数值求解方法。与三维有限元结果相比,所提方法可较为准确地预测管片结构的内力和变形,表现出良好的整体等效性。此外,借助三维接触有限元分析,进一步深入探讨了螺栓孔间隙与衬垫本构关系对管片纵向错台量的影响。     

Chain Conformation and Local Rigidity of Soluble Polyimides(Ⅱ): Isomerized Polyimides in THF

Two soluble isomerized polyimides(PIs)synthesized from 2,2′-bis(3,4-dicarboxyphenyl)hexafluoropropane dianhydride(6FDA)with either 2,2′-dimethylbenzidine(2,2′-DMB)or 3,3′-DMB were investigated by means...     

Geometric lattice actions, entropy and fundamental groups

Let be a lattice in a noncompact simple Lie Group G, where . Suppose acts analytically and ergodically on a compact manifold M preserving a unimodular rigid geometric structure (e.g. a connection and a volume). We show that either the action is isometric or there exists a "large image" linear representation of . Under an additional assumption on the dynamics of the action, we associate to a virtual arithmetic quotient of full entropy. Received: December 14, 2000     

Elastic constants of lead-bismuth alloys

A study of ultrasonic velocities and internal friction has been carried out in Pb-Bi alloys in the concentration range of 0 to 49.5 atomic % Bi using the composite oscillator technique. From the velocity and density data a set of elastic constants namely, Young’s modulus, rigidity modulus, bulk modulus and Poisson’s ratio are estimated. The results are interpreted in terms of the phase changes occurring in the alloy system. Internal friction is found to be more sensitive than the elastic constants to the phase changes.     

On Sobolev infinitesimal rigidity of linear hyperbolic actions on the 2-torus*

Let A be a symmetric hyperbolic matrix in SL(2, ℤ) and Γ the subgroup of SL(2, ℤ) generated by A. We aim to study the infinitesimal rigidity of the standard action of Γ on the torus . More precisely, we will consider the Sobolev W^s–infinitesimal rigidity of this action (that is to determine if the cohomology space H¹(Γ,W^s (T M)) is trivial or not), and show that it is W^s–infinitesimally rigid only if 0 ≤ s < 1. A consequence will be that this action is not C^∞–infinitesimally rigid. *I would like to thank A. El Kacimi for introducing me this problem about which we had many fruitful discussions.     

Decompositions of Fundamental Groups of Closed Surfaces into Free Constructions

Let T be a closed surface. It is proven that any decomposition of ₁(T,x) into an amalgamated product (or, more generally, into the fundamental group of a finite graph of groups) with f.g. edge group(s) is almost geometric. A problem of H. Zieschang is solved and the edge rigidity property is investigated.     

用于测量熔合反应的静电偏转板介绍

An electrostatic deflector for separating the fusion evaporation residues from the beam-like products in heavy ion reactions was installed. The evaporation residue separation and identification with the electrostatic deflector setup was tested with the reaction ^32S＋^96Zr at several energies. The fusion evaporation residues and the beam-like particles were well separated after the electrical separation and the experimental fusion cross section obtained from the angular distribution is in good agreement with the calculated value well above the Coulomb barrier. This confirms the reliability of the setup.     

Flexural rigidity of a liquid surface

The energy of a mass of liquid is evaluated asymptotically in powers of the range of the intermolecular potential divided by a typical dimension of the liquid. The leading term is the internal energy, proportional to the liquid volume. The second term is the energy of surface tension, proportional to the area of the liquid surface. The third term is proportional to an integral over this surface of the square of the mean curvature of the surface minus one-third of its Gaussian curvature. This new term has exactly the form of the bending energy of a thin elastic plate. Comparing it with the bending energy yields expressions for the flexural rigidity and the Poisson ratio of the liquid surface. This flexural rigidity of the surface leads to new terms in the equation of equilibrium of the liquid surface, in addition to the usual surface tension terms.     

Rigidity and regularity in group actions

Some new results on the rigidity of automorphism groups and the regularity of(?)-Neumann operator in group actions are presented.