由5-磺基水杨酸组装的镧系金属有机骨架配合物的合成及结构

在水热条件下,合成了6个新的镧系金属配位聚合物,Ln₂(SSA)₂(phen)₄(H₂O)₂(Ln=Er(Ⅲ) (1),Yb(Ⅲ) (2)),[La₂(SSA)₂(phen)₂(H₂O)₄·(phen)·(H₂O)_{1.33相似文献}   

Azido‐Substituted BODIPY Dyes for the Production of Fluorescent Carbon Nanotubes

A series of azido‐dyes were synthesized through Knoevenagel reactions of an azido‐BODIPY with aromatic aldehydes. The nature of the substituents allowed the fine tuning of their spectroscopic properties. The dyes were used to decorate oxidized multiwalled carbon nanotubes (ox‐MWCNTs), bearing terminal triple bond groups, by CuAAC reactions, affording fluorescent materials. This decoration allowed the efficient determination of the internalization of the ox‐MWCNT derivatives by different model cancer cells, such as MCF7.     

Spectral and Acid–Base Properties of Arylidene Derivatives of Dicyclopentano[b,e]pyridines Serving as Fluorescent pH-Indicators

The bathochromic shifts of absorption and emission bands upon the protonation of dicyclopentano[b,e]pyridines fall in the range of 3500-5500 cm^–1. Both the neutral and protonated forms show high fluorescence quantum yields. The spectral and acid–base properties of these compounds may be varied within a broad range by introducing electron-donor substituents into the aromatic side rings. The protonation constants in the ground and excited states of each of these compounds have similar values as a result of steric hindrance arising upon protonation of the pyridine nitrogen atom.     

Label-free molecular beacon system based on DNAs containing abasic sites and fluorescent ligands that bind abasic sites

A new class of label-free molecular beacon (MB) system based on DNA strands that contain abasic (AP) sites (AP-DNA) and adopt stem-loop structures, in combination with fluorescent ligands that bind these AP sites, has been developed. Unlike a conventional MB, which requires covalent labeling of the MB with a fluorophore and a quencher, the developed system (APMB) does not require covalent attachment of signal transduction units. Detailed sensing functions of a series of APMB systems were examined with the aid of the fluorescent ligand named ATMND to provide insight into the design strategy for APMB systems. The effects of the stem length and the position of the AP site in the stem moiety on the fluorescence response of the APMB system were examined. Genotyping of a G/C SNP of PCR amplification products was successfully demonstrated with the APMB system and blue-fluorescent ATMND as a ligand. The APMB system was further extended to a system that utilized green-fluorescent lumiflavin.     

Fluorescent Protein Capped Mesoporous Nanoparticles for Intracellular Drug Delivery and Imaging

A multifunctional system for intracellular drug delivery and simultaneous fluorescent imaging was constructed by using histidine‐tagged, cyan fluorescent protein (CFP)‐capped magnetic mesoporous silica nanoparticles (MMSNs). This protein‐capped multifunctional nanostructure is highly biocompatible and does not affect cell viability or proliferation. The CFP acts not only as a capping agent, but also as a fluorescent imaging agent. The nanoassembly was activated by histidine‐based replacement, leading to release of drug molecules encapsulated in the nanopores into the bulk solution. The fluorescent imaging functionality would allow noninvasive tracking of the nanoparticles in the body. By combining the drug delivery with cell‐imaging capability, these nanoparticles may provide valuable multifunctional nanoplatforms for biomedical applications.     

High-level expression of soluble human β-defensin-2 fused with green fluorescent protein in Escherichia coli cell-free system

Human β-defensin-2 (hBD2), a small cationic peptide, exhibits a broad range of antimicrobial activity and does not acquire any microbial resistance. To produce this uneasily detectable, degradable, and toxic polypeptide efficiently, an alternative approach based on the Escherichia coli cell-free biosynthesis system was proposed. The approach implies that a polypeptide of interest is synthesized as a fusion protein linked to a green fluorescent protein (GFP) through a cleavable spacer. With batch-mode operation, a significant amount of hBD2 fused with GFP (0.25 mg/mL) can be expressed in this cell-free system. The productivity of the fusion protein can be improved up to 1.2 mg/mL by employing a continuous-exchange cell-free system. Furthermore, the GFP moiety provides directly visible and quantitative monitoring of the polypeptide synthesis, and the product is soluble and stable. This work will be helpful in allowing the rapid and visible expression of other similar defensins using an in vitro cell-free system.     

非晶CS/RB纳米薄膜的荧光性质

采用低真空物理气相沉积法制备单组份氰基对称二苯代乙烯(CS)纳米薄膜和罗丹明B(RB)纳米薄膜以及CS/RB叠层纳米薄膜。使用荧光分光光度计、X射线衍射仪(XRD)和扫描电子显微镜(SEM)表征薄膜样品的荧光性质、结构和表面形貌。与粉末晶态样品相比,薄膜样品的荧光发射峰数量变多,发射波长范围拓宽到120 nm。有机小分子的非晶聚集态和纳米叠层结构的的纳米效应导致了样品的荧光性质的变化。     

稀土有机螯合物发光研究进展

总结了稀土有机螯合物结构与发光性能的关系：配体最低三重态能级与稀土离子激发态能级的匹配是中心稀土离子能否发光的主要因素;螯合物结构的平面性和刚性是中心离子发光效率高低的重要因素;适宜的第二配体的加入一般导致螯合物分子刚性和稳定性增高,因而有利于能量的传递,致使中心离子发光效率增高,但也不能忽视第二配体加入所引起光能的吸收和能量传递过程的竞争;配体的耐热,耐辐射性是配合物能否作为材料的必要因素,自行设计,合成了5类25种新的有机配体及其相应的二元,三元稀土螯合物,研究了这些螯合物的配位性质,发光性能,发光与结构关系及发光机制。提出并发展了稀土离子发光和电子振动光谱作为配合物和生物分子结构探针的两种新的方法。将稀土－β－二酮的发光螯合物与树脂制成荧光塑料;利用铕和铽螯合物的发光和免疫反应,检测了体液中生物活性物质的含量,证实以稀土离子替代放射性同位素作为标记物,有希望替代放射免疫分析方法,成为常规的临床检验方法,利用Tb^3 荧光检测了植物中生长激素的含量。     

The Effect of cis-trans Isomerism on the Electronic-vibrational Structure of the Ground and Excited States and Reactivity of 2-(2-Furyl)- and 2-(2-Thienyl)oxazole

The fluorescent properties, structure, and electronic structure of the ground and excited singlet and triplet electronic states of the cis and trans forms of 4,5-dihydro-2-(2-furyl)oxazole, 4,4-dihydro-2-(2-thienyl)oxazole, 2-(2-furyl)oxazole (FO), and 2-(2-thienyl)oxazole (TO) have been studied. The orbital nature of the lower excited singlet and triplet states has been studied by the semiempirical INDO/S (valence approximation) and PPP/S ( approximation) methods. It was shown that for FO and TO molecules the lower triplet state is of the * type, for which delocalization of the electronic excitation on atoms is characteristic. In the singlet excitation state inversion was observed of the energy levels of the delocalized * states and n* states localized over several bonds (for the free TO and FO molecules the lower excited singlet states S₁* were assigned to * and n* types respectively). Owing to the low position of the T * and T _n* levels relative to the singlet level of * type, the rate constant for intercombination conversion is greater than the rate constant for radiative decay. Consequently an efficient population of the triplet states of the molecules occurs under conditions of electronic-vibrational excitation. The direction of reactions during synthesis was compared with the localization indices in the ground state for electrophilic, nucleophilic, and radical substitution, and also with the excitation localization numbers L for a wide selection of electronically excited states. It was concluded that the change in the structure of the azole molecule on replacing an O atom by an S atom, or on changing from a partially hydrogenated to a heteroaromatic system, was the main reason for the change of all the spectral parameters characterizing the electronic-vibrational or the spin-orbital interaction of the most reactive groups of atoms in the molecular structure.