HCSE method for detection of small carbon–carbon couplings and their signs,comparison with SLAP pulse sequence

Performance of homonuclear coupling sign edited (HCSE) experiment applied to detection of signed carbon–carbon couplings is discussed using a set of already measured samples of nine monosubstituted benzenes. It is shown that coupling sign detection is insensitive to the settings of carbon–carbon polarization transfer delays. The HCSE spectra of ten from the total of 43 measured carbon–carbon couplings were considerably influenced by relaxations and proton–proton strong couplings. These effects are quantitatively discussed. The results of HCSE and SLAP experiments are compared. It is shown that the two methods may complement each other in detection of signed carbon–carbon couplings. Copyright © 2013 John Wiley & Sons, Ltd.     

Selective lighting up of segments around Gly,Ala and Ser/Thr in proteins

Direct detection of ¹³C nucleus can be used as a valuable alternative where ¹H detection poses a challenge due to relaxation effects, chemical exchange and poor chemical shift dispersion. In this context, we have designed a suite of 2D ¹³C^α‐detected hNCA experiments that provide sequential correlations of ¹³C^α with ¹⁵N on one hand and efficient spectroscopic labeling of certain groups of residues, namely, Gly, Ala, Ser and Thr, on the other. These residues act as checkpoints in the sequential walk, which in turn offer new possibilities of backbone assignment of small proteins from a set of 2D experiments, thereby providing great economy in terms of spectrometer time. The direct identification of peptide segments around Gly, Ala, Ser and Thr residues along a protein chain will be highly valuable for deriving important information on sites of ligand binding, phosphorylation, inhibitor/substrate binding, understanding protein folding pathways, comprehending local conformational dynamics etc. without having to obtain complete sequence‐specific assignments, which can be time consuming and at times formidable, especially in large proteins. We have illustratively demonstrated the multifaceted applications of these variants of 2D experiments on ubiquitin and M‐crystallin. We foresee that these 2D hNCA experiments will provide economic and efficient strategies for studying the structure and function of proteins. Copyright © 2012 John Wiley & Sons, Ltd.     

盐生盐杆菌灭活原生质体融合的研究

通过双亲灭活方法将二株具不同遗传学特性的盐生盐杆菌Ｊ７（含质粒，不产紫膜）和Ｒ１（产紫膜，不含质粒）进行原生质体融合试验．结果表明，在以３０％ＰＥＧ６００为助融剂，加２５ｍｍｏｌ·Ｌ－１ＥＤＴＡ条件下，４０℃保温１ｍｉｎ，获得融合率为０．７６×１０－５的融合子．这些融合子具有双亲菌株的某些特征，其中２２％的融合子既含有质粒又产紫膜，这些融合子对于进一步研究质粒与紫膜的关系，以及筛选高产紫膜的优良菌株，均有十分重要的价值．     

答YapH.P.和TeoS.K.问题

本文证得：如果Ｆ是Ｃｎ中的一条种路，则Ｇ中同时通过ｋ余弦ｅ１，ｅ２，．．．，ｅｋ而不通过Ｆ中的任一条边的圈最多只有一个且Ｇ中同时通过ｋ条弦ｅ１，ｅ２，．．．，ｅｋ的圈最多只有２个，进而由之给出了Ｍ（ｋ）的上界和ｍ（ｋ）的下界及ｍ（ｋ）＝（ｋ＋１）（ｋ＋２）／２成立的一个条件，否定地回答了ＹａｐＨ．Ｐ．和ＴｅｏＳ．Ｋ．１９８４年提出的一个问题。     

Integrable Hamiltonian systems related to the Hilbert–Schmidt ideal

By the application of the coinduction method as well as the Magri method to the ideal of real Hilbert–Schmidt operators we construct the hierarchies of integrable Hamiltonian systems on Banach Lie–Poisson spaces which consist of such types of operators. We also discuss their algebraic and analytic properties and solve them in dimensions, N=2,3,4$N=2,3,4$.