Fluorogenic Probes Applied to the Study of Induced Oxidative Stress in the Human Leukemic HL60 Cell Line

Development of microspectrofluorometric methods using specific fluorogenic probes has provided precious help in studying in situ oxidative stress and cellular protective systems. The aim of this study was to determine ROS production concomitantly with a modification of the intracellular thiol pool after applying an oxidative stress to a nonadherent cell model represented by the HL60 cell line. The dichlorodihydrofluorescein diacetate (H₂DCFDA) probe assessed the kinetic production of ROS by cells submitted to the chemical oxidant t-butylhydroperoxide with a high signal/noise ratio. The probe sensitivity permitted us to detect endogenous ROS production in HL60 cells and the protective effect of N-acetyl cysteine against ROS. The chloromethylfluorescein diacetate probe (CMFDA) permitted us to evaluate the thiol depleting effect of N-ethyl maleimide. Complete thiol depletion was associated with a moderate increase in ROS production. The cell viability was determined with calcein-AM, which gave results similar to those with the tetrazolium dye. This probe was not affected by intracellular pH and did not required an extraction step, unlike tetrazolium dye. In conclusion, cell-permeant fluorogenic probes are useful and sensitive tools to determine in situ ROS production concomitantly with consecutive change in the thiol system in a living and non-adherent cell model.     

Exponential time integration for fast finite element solutions of some financial engineering problems

We consider exponential time integration schemes for fast numerical pricing of European, American, barrier and butterfly options when the stock price follows a dynamics described by a jump-diffusion process. The resulting pricing equation which is in the form of a partial integro-differential equation is approximated in space using finite elements. Our methods require the computation of a single matrix exponential and we demonstrate using a wide range of numerical tests that the combination of exponential integrators and finite element discretisations with quadratic basis functions leads to highly accurate algorithms for cases when the jump magnitude is Gaussian. Comparison with other time-stepping methods are also carried out to illustrate the effectiveness of our methods.     

Generalised Kummer constructions and Weil restrictions

We use a generalised Kummer construction to realise all but one known weight four newforms with complex multiplication and rational Fourier coefficients in Calabi-Yau threefolds defined over Q. The Calabi-Yau manifolds are smooth models of quotients of the Weil restrictions of elliptic curves with CM of class number three.     

Analysis of diffusive two-competing-prey and one-predator systems with Beddington-Deangelis functional response

In this paper, a diffusive two-competing-prey and one-predator system with Beddington-DeAngelis functional response is considered. The sufficient and necessary conditions for the existence of coexistence states are provided using the fixed point index theory developed. In addition, the stability and uniqueness of coexistence states are investigated. Finally, this paper discusses the sufficient conditions for extinction and permanence of the time-dependent system.     

Multidimensional continued fractions and a Minkowski function

The Minkowski question mark function can be characterized as the unique homeomorphism of the real unit interval that conjugates the Farey map with the tent map. We construct an n-dimensional analogue of the Minkowski function as the only homeomorphism of an n-simplex that conjugates the piecewise-fractional map associated to the M?nkemeyer continued fraction algorithm with an appropriate tent map. Author’s address: Department of Mathematics, University of Udine, via delle Scienze 208, 33100 Udine, Italy     

Discrete Galerkin method for Fredholm integro-differential equations with weakly singular kernels

Approximations to a solution and its derivatives of a boundary value problem of an nth order linear Fredholm integro-differential equation with weakly singular or other nonsmooth kernels are determined. These approximations are piecewise polynomial functions on special graded grids. For their finding a discrete Galerkin method and an integral equation reformulation of the boundary value problem are used. Optimal global convergence estimates are derived and an improvement of the convergence rate of the method for a special choice of parameters is obtained. To illustrate the theoretical results a collection of numerical results of a test problem is presented.     

Almost sure and moment exponential stability of Euler–Maruyama discretizations for hybrid stochastic differential equations

Positive results are derived concerning the long time dynamics of numerical simulations of stochastic differential equation systems with Markovian switching. Euler–Maruyama discretizations are shown to capture almost sure and moment exponential stability for all sufficiently small timesteps under appropriate conditions.     

Analysis of variance and linear contrasts in experimental design with generalized secant hyperbolic distribution

We consider one-way classification model in experimental design when the errors have generalized secant hyperbolic distribution. We obtain efficient and robust estimators for block effects by using the modified maximum likelihood estimation (MML) methodology. A test statistic analogous to the normal-theory F statistic is defined to test block effects. We also define a test statistic for testing linear contrasts. It is shown that test statistics based on MML estimators are efficient and robust. The methodology readily extends to unbalanced designs.     

Sharp concentration of the chromatic number on random graphsG n,p

The distribution of the chromatic number on random graphsG _{n, p} is quite sharply concentrated. For fixedp it concentrates almost surely in √n ω(n) consecutive integers where ω(n) approaches infinity arbitrarily slowly. If the average degreepn is less thann ^1/6, it concentrates almost surely in five consecutive integers. Large deviation estimates for martingales are used in the proof.