HCVNS4基因在大肠杆菌中表达及蛋白质提纯

应用PCR方法扩增出人丙型肝炎病毒全长NS4基因的DNA片段,克隆入表达载体pQE32并转化E.coliJM109.经IPTG诱导表达出42×l03蛋白.利用Ni-NTA-agarose纯化得到纯化产物,Western-blot鉴定该蛋白能够与抗NS4的单克隆抗体发生特异性反应.     

Two Approaches for Indirect Teleportation with Partially Entangled States

Teleportation of a qubit with two partially entangled qubit states linking three nodes as quantum channels is extensively studied via the usual ancilla method. With the method two realization approaches, i.e., the node progression approach and the global accumulation approach, are presented. Their resource consumptions, operation complexities, and el~ciencies axe evaluated and compared. It is found that the latter approach is better than the former one besides the error is partially self-corrected. The latter approach is further improved so that two merits are resultant. The improved version is compared with a similar protocol [M.Y. Wang and F.L. Yah, Eur. Phys. J. D 54 （2009） 111]. Their merits and additional costs are exposed.     

高活性铈银介孔复合抗菌剂的制备

利用分步浸渍法制备了Ce银介孔复合无机抗菌剂,并对其进行XRD,TEM,N2吸脱附等表征,结果显示,该抗菌剂仍然保持有序介孔结构,活性物种Ag以纳米线状稳定存在于孔道内。运用抑菌圈法和最小抑菌浓度法对其抗菌性能进行了评价,测试表明,该样品对金黄色葡萄球菌和大肠杆菌均有着优良的抑杀效果。这是由于抗菌活性物种Ag高度分散在介孔材料孔道中,以及Ce有促进Ag＋产生羟基自由基,因而能强化Ag的抗菌性能的原因。     

单细胞激光拉曼光谱检测重组大肠杆菌细胞表达甲酸脱氢酶

甲酸脱氢酶(FDH,EC1.2.1.2)在工业生产中有重要的应用价值,工业上应用的FDH可以通过构建高水平表达重组FDH蛋白的基因工程菌生产,用分子生物学的方法检测重组蛋白的高效表达和积累操作繁杂,耗时耗力且需要破碎细胞。为了寻找一种简单快速,不需破碎细胞,且能实时检测FDH重组蛋白在基因工程菌中表达情况的方法,本研究应用单细胞激光拉曼光谱分析技术(LTRS),研究IPTG诱导不同时间后甲酸脱氢酶重组蛋白(FDH)在大肠杆菌细胞中的表达水平。结果表明,FDH的特征峰1004,1355,1455和1667cm-1随着IPTG诱导时间的延长而增强,说明在诱导培养过程中FDH重组蛋白在重组大肠杆菌细胞中表达并积累,这4个峰强的增加值所反映的FDH表达量与SDS-PAGE电泳分析结果一致。实验结果证明,LTRS是快速有效检测单个大肠杆菌活细胞体内重组FDH实时表达的一种非入侵方法。     

大肠杆菌基于微波法制备的纳米银胶的SERS光谱研究

在微波法制备的纳米银上获得了大肠杆菌的表面增强拉曼光谱, 大肠杆菌在650、952、1125、1242、1320、1372、1459 cm-1有明显的拉曼振动峰。其中650 cm-1处的振动峰最强, 1124、1320、1372和1459 cm-1处的四个振动峰较弱, 952、1242 cm-1处的二个振动峰强度居中。位于650 cm-1附近的拉曼峰源自于酪氨酸、鸟嘌呤的振动, 952 cm-1属于缩氨酸基团C=C的伸缩振动,1124 cm-1是蛋白质的C-N和C-C伸缩振动引起的, 1242 cm-1则归属于是酰胺 III蛋白的振动, 1320 cm-1是蛋白质的CH变形振动, 1372 cm-1是由酪氨酸振动引起的, 1459 cm-1则是蛋白质的CH2变形振动。     

Vector meson rescattering effect in understanding the threshold enhancement observed in J/ψ→γp

We propose one possible mechanism,i.e.,the vector meson （VV） rescattering effects,to interpret the near threshold narrow enhancement observed in J/ψ → γpp.The estimate indicates that these effects can give sizeable contributions to this channel,and a destructive interference between different rescattering amplitudes is required to reproduce the line shape of the data.     

Quark-clustering in cold quark matter

Quarks are proposed to be grouped together to make quark-clusters due to the strong interaction in cold quark matter at a few nuclear densities,because a weakly coupling treatment of the interaction between quarks there would be inadequate.Cold quark matter is then conjectured to be in solid state （i.e.,forming a crystal structure） if the inter-cluster potential is deep enough to localize clusters in lattice.Such a solid state of cold quark matter would be very necessary for us to understand different manifestations of pulsar-like compact stars,and could not be ruled by first principles.     

Light-Induced Agglomeration and Diffusion of Different Particles with Optical Tweezers

The dynamic process of light-induced agglomeration of carbon nanotubes （CNTs）, C60 and Escherichia coli （E.eoli） in aqueous solutions is demonstrated using an optical tweezers system. Based on the results, the diameter of the agglomerated region and the agglomeration rate increase with the increasing laser power. After the saturation-stable period, CNTs diffuse completely, C60 clusters only diffuse partially, and E. coli never diffuses in the agglomeration region. Theoretical analyses show that the molecular polarization and thermal diffusion of particles play crucial roles in the diffusion process. The and sort nanoparticles. results indicate the possibility of using light to aggregate     

New Type Soliton Solutions to Korteweg-de Vries and Benjamin-Bona-Mahony Equations

We study the Korteweg-de Vries equation and the Benjamin-Bona-Mahony equation, and obtain three kinds of new type soliton solutions, i.e. peakon solutions, double-peak （peaked-point and peaked-compacton） soliton solutions. A double solitary wave with blow-up points is also contained.