Proteins play a central role in all domains of life, and precise regulation of their activity is essential for understanding the related biological processes and therapeutic functions. Nucleic acid aptamers, the molecular recognition components derived from systematic evolution of ligands by exponential enrichment(SELEX), can specifically identify proteins with antibody-like recognition characteristics and help to regulate their activity. This minireview covers the SELEX-based selection of protein-binding aptamers, membrane protein analytical techniques based on aptamer-mediated target recognition, aptamer-mediated functional regulation of proteins, including membrane receptors and non-membrane proteins(thrombin as a model), as well as the potential challenges and prospects regarding aptamer-mediated protein manipulation, aiming to supply some useful information for researchers in this field. 相似文献
Continuous and pulsed 12 keV electron beams were used to excite nitrogen
within a gas cell at pressures ranging from 10 to 1400 hPa. The pressure
dependence of the ratio of photon fluxes for emission from vibrational
levels v'=0 and 1 of the C 3Π u state has been studied. The
results confirm the presence of a collisional excitation mechanism
populating v'=0, 1 in addition to electron impact excitation. Rate constants
of (1.27 ±0.04)×10-11 cm3s-1 [ v'=0] and
(2.68 ±0.08)×10-11 cm3s-1 [ v'=1] were measured for
C 3Π u quenching by ground state nitrogen. For electron beam
conditions relative excitation efficiencies of 1:0.59:0.22 for
vibrational levels 0, 1 and 2 were calculated. The recorded flux ratios are
compared with the predictions given by a vibrational relaxation model. 相似文献
The final state particle multiplicity distributions in high-energy nucleus-nucleus collisions are described by two different sub-distributious contributed by a single nucleon.The Monte Carlo calculated results from the two sub-distributions and the participant-spectator model are compared and found to be in agreement with the experimental data of Au-Au collisions ats=130 AGeV and Pb-Pb collisions at 158 AGeV. 相似文献
We analyze the approximation and smoothness properties of quincunx fundamental refinable functions. In particular, we provide a general way for the construction of quincunx interpolatory refinement masks associated with the quincunx lattice in . Their corresponding quincunx fundamental refinable functions attain the optimal approximation order and smoothness order. In addition, these examples are minimally supported with symmetry. For two special families of such quincunx interpolatory masks, we prove that their symbols are nonnegative. Finally, a general way of constructing quincunx biorthogonal wavelets is presented. Several examples of quincunx interpolatory masks and quincunx biorthogonal wavelets are explicitly computed.
An optimum configuration of the discharge channel of a coaxial Hcompressed discharge is determined to exclude the screening influence of an envelope on discharge radiation in the UV region of a spectrum. It is shown that the use of an Hcompressed discharge of coaxial shape makes it possible to increase the radiation yield in the UV spectrum as compared to a plane shape at the same parameters of electric power supply. 相似文献