猪饲料中甲硝唑、二甲硝咪唑药物含量的HPLC法测定

样品经氯仿提取后 ,用 Meta Chem Polaris 5μ C18- A柱分离 ,二极管阵列检测器紫外检测、外标法定量 ,高效液相色谱法同时测定猪饲料中甲硝唑、二甲硝咪唑含量。甲硝唑、二甲硝咪唑的检出限均为0 .4 0 mg/ kg,回收率分别大于 71%和 82 % ,RSD分别小于 5 .2 %和 6 .3%。     

火焰原子吸收光谱法连续测定饲料中的铅和镉

火焰原子吸收法连续测定饲料中的铅和镉 ,方法灵敏、准确。无须富集、萃取 ,操作简便 ,铅和镉的回收率分别在 95 .0 %— 1 0 5 .0 %和 94 .0 %— 98.0 %之间 ,相对标准偏差分别在 0 .4 8%— 1 .4 0 %和 2 .5 6 %—3.5 0 %之间     

毛细管电泳电导法快速检测饲料添加剂氯化胆碱

采用毛细管电泳电导法对饲料添加剂氯化胆碱进行了测定.实验优化了缓冲介质、分离电压、毛细管长度和内径等参数.结果表明:在优化条件下,氯化胆碱在4 min左右可以实现分离检测,线性范围在41.8～1.20 μg/mL,检出限为0.8 μg/mL;迁移时间和峰面积的相对标准偏差分别为0.6%、2.1%;样品加标回收率为97%～105%.将该法用于粉剂和饲料中氯化胆碱的检测,取得了满意的结果.     

Feed spacer mesh angle: 3D modeling, simulation and optimization based on unsteady hydrodynamic in spiral wound membrane channel

Basic knowledge on the hydrodynamics in the spacer-filled spiral wound membrane (SWM) channel is vital for the understanding of the formation of concentration polarization at the membrane interface. In the present study, a 3D laminar transient hydrodynamics modeling approach was used to study and optimize the spacer mesh angle for the SWM feed spacer. Based on the simulated results, the optimal spacer mesh angle that yields the minimum effective concentration polarization factor, was found to be α120β30. Under this optimal mesh angles, spacer α120β30 also demonstrated the highest magnitude of unsteady hydrodynamics (which adjacent to the membrane wall) at a moderate degree of pressure loss.     

化学教育专业开设生物化学课程的教学改革研究

结合生物化学理论性强、内容抽象、知识更新快等特点, 对在师范类化学教育专业开设生物化学课程的必要性,以及教学内容和教学方法的改革进行了探讨。     

MOOC环境下生物化学微课程的设计

尝试了在生物化学教学过程中应用MOOC协同教学模式进行教学重构,并以脂肪分解代谢一节为例着重详述慕课核心——微课程的设计过程,这种新颖的教学模式能显著提高学生的学习兴趣,也为研究者参与 MOOC 环境教学提供参考。     

自适应模糊PID前馈补偿在机载挂飞摆扫转台控制中的应用

针对机载挂飞转台的摆扫速度控制问题,提出了一种利用模糊自适应PID技术进行前馈补偿的复合控制策略。首先根据实际应用提出摆扫转台的期望摆扫速度曲线,并对直流力矩电机驱动的摆扫转台进行了建模;然后根据扰动前馈补偿的控制原理,提出了模糊自适应PID前馈补偿方法,为摆扫转台的速度环设计了模糊PID控制器,并在此基础上设计了与之相适应的的自适应前馈补偿函数;最后进行了仿真结果验证。通过Matlab仿真结果表明,相对于模糊PID控制,所设计的模糊自适应PID前馈补偿控制器能有效的跟踪期望的转台摆扫速度,大幅地提高了在有稳定干扰和摆扫速度越变情况下的跟踪精度。     

STM and AFM of bio/organic molecules and structures

Applications of scanning tunneling and atomic force microscopes in bio/organic researches are reviewed with a special emphasis on the types of researches that are expected to contribute to the creation of a new field of “single molecule biochemistry” in the near future. The reviewed articles within the scope as stated above actually include a fairly broad spectrum of researches. It is, therefore, a hope of the author that this review will be useful to those who are considering biological applications of the probe microscopy techniques but are not quite familiar with the types of experiments that have been done in the past. In the section on scanning tunneling microscopy, identification of chemically distinct functional groups by the difference in their tunneling properties will be discussed as a main focus because it is fundamental for biochemists to identify molecules by their shapes and properties. In the section on atomic force microscopy, recent progresses in the imaging techniques of proteins and DNAs are closely reviewed, and rapidly advancing technologies of single molecule measurements and manipulation of nanometer sized structures are given extensive coverage because the author considers that such new applications are extremely promising to open an entirely new field in biological sciences.     

植物次生化合物光谱分析预测动物采食量研究进展

植食动物采食量的研究对提高动物生产力以及野生动植物保护有重要的意义,但长期以来对放牧动物和野生动物一直缺乏比较准确的采食量预测方法。植物次生化合物及其代谢物与动物采食量有相关关系,动物排泄物中次生代谢物种类及含量取决于其采食的植物次生化合物。应用波谱分析技术,对动物排泄物中的次生代谢物及其在植物中的前体物(对应的植物次生化合物)进行提取、分离和纯化、结构鉴定,并进行量化分析,建立次生代谢物与采食量之间的回归相关关系和浓度-比率模型,可以准确地预测放牧家畜和野生动物的采食量。最具预测采食量潜力的一类次生化合物是芳香族化合物及其衍生物,并已经在利用光谱技术的基础上,以单宁、链烷、类黄酮等作内源标记物预测采食量方面取得了进展。提出了存在问题和需进一步研究的方向。