Study on the Synthesis and in vitro Photodynamic Anti-cancer Activity of Tetra（trifluoroethoxy） Germanium Phthalocyanine①

The synthesis and in vitro photodynamic anticancer activity of a new photosensitizer, tetra(trifluoroethoxy) germanium phthalocyanine(GePcF), were studied. GePcF was characterized by UV-Vis, IR, MS and elemental analysis. The in vitro photodynamic activity of GePcF was studied by MTT. IC50 of GePcF for SW480 cells of human colonic adenocarcinoma and HeLa cells of cervical cancer were 36.53 and 45.78 μmol/L, respectively. GePcF as a photosensitizer may be used to treat cancers due to its photodynamic anticancer activity.     

Synthesis,characterization and cytotoxicity of fluorescent organotin complexes of terpyridine derivatives

Five organotin complexes of terpyridine derivatives were synthesized and characterized. The mononuclear Sn（IV） complexes were six-coordinated adopting a distorted octahedral coordination geometry. A brief outline of the fluorescence spectra and the in vitro cytotoxicity of the Sn（IV） complexes have been given.     

Design,synthesis and biological evaluation of E-ring modified evodiamine derivatives as novel antitumor agents

A series of novel E-ring modified evodiamine derivatives were designed and synthesized as antitumor agents. Their capacity to interfere with the catalytic activity of topoisomerase Ⅰ and Ⅱ was evaluated by the relaxation assay. In vitro antitumor activity results revealed that compound 12 showed good antitumor activity with a broad spectrum. Its binding modes with topoisomerase Ⅰ and Ⅱ were clarified by molecular docking.     

微量元素锌和钙对大鼠颅盖骨成骨细胞样细胞体外培养增殖分化的影响

所用的细胞按照Luben改良的方法培养。用S．D．大鼠颅盖骨，以胶原酶、胰蛋白酶消化，收集细胞，在10％胎牛血清的199培养液中。于5％CO3、37℃饱和湿度培养，在条件液中。分别加不同浓度的微量元素锌和钙，从形态学及生化指标探讨，证实微量元素锌和钙对成骨细胞样细胞的增殖分化都有明显的髟响。     

体外冲击波粉碎肾结石

著者在设计并完成了水中聚焦冲击波高体粉碎肾结石后,又对聚焦冲击波对动物组织的影响进行了实验研究,结果表明对动物组织无损伤,于是进行了大动物实验,体外冲击波粉碎狗肾脏中的结石(人工植入)获得成功,在上述工作的基础上,取得了临床体外碎石六例的成功。在X光-电视交叉定位系统中,采用了IBM-PC微机进行图像处理。     

温敏性壳聚糖共聚膜的制备与细胞吸附/脱附行为

将丙烯酸(AAc)与壳聚糖(CS)反应, 合成了壳聚糖大单体(CS-AAc), 再用CS-AAc与N-异丙基丙烯酰胺(NIPAAm)共聚, 制备P(CS-AAc-NIPAAm)共聚物. 通过红外光谱和X射线光电子能谱等分析证实了产物的结构和组成. 对P(CS-AAc-NIPAAm)共聚膜的动态接触角及对细胞的吸附与脱附行为研究发现, 共聚膜表现出良好的温度敏感性, 其表面成功地种植了成纤维细胞(L929). 当环境温度降低后, 共聚膜表面细胞自动脱附, 从而避免了使用酶解法脱附细胞造成的细胞功能损伤.     

介孔硅基干凝胶的降解性及其生物相容性研究

本研究利用改进的溶胶-凝胶工艺合成了介孔硅基干凝胶。通过差热-热重(DTA-TG)、X-射线衍射(XRD)、红外光谱(FTIR)和N₂等温吸附-脱附测试分析,以及扫描电镜(SEM)、透射电镜(TEM)观察,表征了硅基干凝胶的热效应、相态、结构、形貌特征,并对合成的硅基干凝胶进行了体外降解与细胞培养实验研究。测试结果表明：合成的硅基干凝胶为平均孔径3nm左右、最大比表面积901 m²·g^-1的介孔结构。体外模拟体液(Simulated body fluid,SBF)降解结果显示,低温处理的材料在42 d内完全降解,直线拟合符合一级反应动力学方程。通过添加钙磷成分或高温煅烧处理可以调控干凝胶的降解速率,使硅基干凝胶的降解呈现直线式匀速降解的特征。体外细胞培养表明：硅基干凝胶可促进C2C12细胞(C3H小鼠肌原细胞)的分化和繁殖,证明材料的生物相容性良好。     

新的五羟色胺转运蛋白二芳基醚类衍生物配体的合成与体外评价

为开发新的配体作为有潜力的五羟色胺转运蛋白（SERT）显像剂,合成了一系列二芳基醚类衍生物,并对这些化合物进行了表征,测定了对SERT的体外亲和性,其中,化合物2-（2-（（dimethylamino）methyl）-4-fluorophenoxy）-5-bromobenzeneamine（15）和2-（2-（（dimethylamino）-methyl）-4-fluorophenoxy）-5-iodobenzeneamine（16）表现出最高的亲和性,K分别为0.28和0．20nmol·L^-1它们可以用碳-11、氟-18、碘-123或者溴-76进一步标记,进而评价作为SERT显像剂的可能性。而且,构效关系的研究为将来设计新的配体提供了一些有用的信息。